GAS6 Polyclonal antibody

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  WB analysis of human urine using 13795-1-AP

  human urine sample were subjected to SDS PAGE followed by western blot with 13795-1-AP (GAS6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 13795-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13795-1-AP (GAS6 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of DU 145 using 13795-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 13795-1-AP (GAS6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 13795-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13795-1-AP (GAS6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 13795-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 13795-1-AP (GAS6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human spleen using 13795-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 13795-1-AP (GAS6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human spleen using 13795-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 13795-1-AP (GAS6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 13795-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 13795-1-AP (GAS6 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

GAS6 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 13795-1-AP

Publications(14)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

AXLLG, AXSF, GAS 6, GAS-6, growth arrest 6

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Tested Applications

• Positive WB detected in: human urine sample, DU 145 cells, mouse heart tissue, rat heart tissue
• Positive IHC detected in: human lung cancer tissue, human spleen tissue, mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 10 publications below
• IHC: See 3 publications below
• IF: See 5 publications below

Product Information

13795-1-AP targets GAS6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4617

  Product name: Recombinant human GAS6 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 336-678 aa of BC038984

  Sequence: FAGGHQDSTWIVLALRAGRLELQLRYNGVGRVTSSGPVINHGMWQTISVEELARNLVIKVNRDAVMKIAVAGDLFQPERGLYHLNLTVGGIPFHEKDLVQPINPRLDGCMRSWNWLNGEDTTIQETVKVNTRMQCFSVTERGSFYPGSGFAFYSLDYMRTPLDVGTESTWEVEVVAHIRPAADTGVLFALWAPDLRAVPLSVALVDYHSTKKLKKQLVVLAVEHTALALMEIKVCDGQEHVVTVSLRDGEATLEVDGTRGQSEVSAAQLQERLAVLERHLRSPVLTFAGGLPDVPVTSAPVTAFYRGCMTLEVNRRLLDLDEAAYKHSDITAHSCPPVEPAAA

• Full Name: growth arrest-specific 6
• Calculated Molecular Weight: 678 aa, 75 kDa
• Observed Molecular Weight: 75 kDa
• GenBank Accession Number: BC038984
• Gene Symbol: GAS6
• Gene ID (NCBI): 2621
• RRID: AB_2756522
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q14393
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Growth arrest-specific protein 6 (GAS6) is also named as AXLLG. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses (PMID:12364394, PMID:18840707). It can bridge virus envelope phosphatidylserine to the TAM receptor tyrosine kinase Axl to mediate viral entry by apoptotic mimicry (PMID:21501828).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Int J Cancer; Therapeutic Activity of DCC-2036, a Novel Tyrosine Kinase Inhibitor, against Triple-Negative Breast Cancer Patient-Derived Xenografts by Targeting AXL/MET.; Authors - Yingying Shen
human	WB	Front Cell Infect Microbiol; Persistent Exposure to Porphyromonas gingivalis Promotes Proliferative and Invasion Capabilities, and Tumorigenic Properties of Human Immortalized Oral Epithelial Cells.; Authors - Fengxue Geng
human	WB	FASEB J; Anti-human serum albumin autoantibody may be involved in the pathogenesis of autoimmune bullous skin diseases.; Authors - Hua Qian
human	WB,IHC	Cancer Gene Ther; Targeting LRIG2 overcomes resistance to EGFR inhibitor in glioblastoma by modulating GAS6/AXL/SRC signaling.; Authors - Minhai Dong
mouse	WB	Transplantation; Activated AXL Protects Against Hepatic Ischemia-reperfusion Injury by Upregulating SOCS-1 Expression.; Authors - Zhen Wang
human	WB	Mol Immunol; The role of the Gas6/TAM signal pathway in the LPS-induced pulmonary epithelial cells injury; Authors - Yujing Cheng