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  • KD/KO Validated

GATA3 Polyclonal antibody

GATA3 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ChIP

Conjugate

Unconjugated

Cat no : 10417-1-AP

Synonyms

GATA binding factor 3, GATA binding protein 3, GATA3, HDR



Tested Applications

Positive WB detected inMCF7 cells, HeLa cells, MCF-7 cells
Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10417-1-AP targets GATA3 in WB, IHC, ChIP applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GATA3 fusion protein Ag0174
Full Name GATA binding protein 3
Calculated molecular weight 443 aa, 48 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberBC003070
Gene symbol GATA3
Gene ID (NCBI) 2625
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GATA3 is a transcriptional factor that binds to the enhancer elements of all 4 T-cell antigen receptor genes. GATA3 is highly expressed in naive, freshly activated cells and Th2 lineage cells and thought to be necessary and sufficient for Th2 cytokine gene expression. GATA3 and TBET interfer with each other to regulate gene expression. This is a rabbit polyclonal antibody raised against part of C-terminus of human GATA3.

Protocols

Product Specific Protocols
WB protocol for GATA3 antibody 10417-1-APDownload protocol
IHC protocol for GATA3 antibody 10417-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human, mouseWB

Cancer Immunol Res

CD4 T Cells Require ICOS-Mediated PI3K Signaling to Increase T-Bet Expression in the Setting of Anti-CTLA-4 Therapy.

Authors - Hong Chen
mouseWB

Biosci Rep

Bronchial epithelial cells of young and old mice could directly regulate the differentiation of Th2 and Th17.

Authors - Da Liu
humanWB

Acta Biochim Biophys Sin (Shanghai)

mTOR pathway regulates the differentiation of peripheral blood Th2/Treg cell subsets in patients with pemphigus vulgaris.

Authors - Kuan Lai
humanIF, WB

ACS Biomater Sci Eng

Microenvironment Can Induce Development of Auditory Progenitor Cells from Human Gingival Mesenchymal Stem Cells.

Authors - Sevda Pouraghaei
humanWB

Oncol Lett

Effects of Helicobacter pylori on the expression levels of GATA-3 and connexin 32 and the GJIC function in gastric epithelial cells and their association by promoter analysis.

Authors - Lihua Huang
  • KD Validated
mouseIHC

Evid Based Complement Alternat Med

Huganbuzure Granule Attenuates Concanavalin-A-Induced Immune Liver Injury in Mice via Regulating the Balance of Th1/Th2/Th17/Treg Cells and Inhibiting Apoptosis.

Authors - Mengheng Wang