GCGR Polyclonal antibody

GCGR Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 26784-1-AP

Synonyms

GCGR, GGR, GL R, glucagon receptor

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Validation Data Gallery

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  WB analysis of mouse liver using 26784-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 26784-1-AP (GCGR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 26784-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 26784-1-AP (GCGR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 26784-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26784-1-AP (GCGR antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 26784-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26784-1-AP (GCGR antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 26784-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 26784-1-AP (GCGR antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 26784-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 26784-1-AP (GCGR antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse liver tissue, mouse heart tissue
• Positive IHC detected in: human liver tissue, human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

26784-1-AP targets GCGR in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GCGR fusion protein Ag24861
• Full Name: glucagon receptor
• Calculated Molecular Weight: 477 aa, 54 kDa
• Observed Molecular Weight: 62 kDa
• GenBank Accession Number: BC104854
• Gene Symbol: GCGR
• Gene ID (NCBI): 2642
• RRID: AB_2880634
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Glucagon receptor (GCGR) is a secretin-like (class B) family of G-protein coupled receptors (GPCRs). It plays an important role in elevating the glucose concentration in the blood and has thus become one of the promising therapeutic targets for the treatment of type 2 diabetes mellitus (PMID: 26236379). GCGR is a 62-kDa glycoprotein that contains at least four N-linked oligosaccharide chains (PMID: 2174441; 15245877). Defects in the gene of GCGR are a cause of non-insulin-dependent diabetes mellitus (NIDDM).

Publications

Species	Application	Title
mouse	WB,IF	iScience; Pro-α-cell-derived β-cells contribute to β-cell neogenesis induced by antagonistic glucagon receptor antibody in type 2 diabetic mice.; Authors - Xiaona Cui
mouse	WB	Mol Cell Endocrinol; Mof acetyltransferase inhibition ameliorates glucose intolerance and islet dysfunction of type 2 diabetes via targeting pancreatic α-cells.; Authors - Xinghong Guo
mouse	WB	Toxicology; Tributyltin exposure disturbs hepatic glucose metabolism in male mice.; Authors - Jing Xu
mouse	WB,IF	Am J Physiol Endocrinol Metab; Glucagon receptor blockage inhibits β-cell dedifferentiation through FoxO1; Authors - Kangli Wang
mouse	IHC,WB	Biomed Pharmacother; Si-Miao-Yong-An decoction preserves cardiac function and regulates GLC/AMPK/NF-κB and GLC/PPARα/PGC-1α pathways in diabetic mice; Authors - Lin Li