Tested Applications
| Positive WB detected in | mouse brain tissue, mouse kidney tissue, rat brain tissue, rat kidney tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
31493-1-AP targets GDA in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag35661 Product name: Recombinant human GDA protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 27-252 aa of BC053584 Sequence: EVLRDHLLGVSDSGKIVFLEEASQQEKLAKEWCFKPCEIRELSHHEFFMPGLVDTHIHASQYSFAGSSIDLPLLEWLTKYTFPAEHRFQNIDFAEEVYTRVVRRTLKNGTTTACYFATIHTDSSLLLADITDKFGQRAFVGKVCMDLNDTFPEYKETTEESIKETERFVSEMLQKNYSRVKPIVTPRFSLSCSETLMGELGNIAKTRDLHIQSHISENRDEVEAVK Predict reactive species |
| Full Name | guanine deaminase |
| Observed Molecular Weight | 48-53 kDa |
| GenBank Accession Number | BC053584 |
| Gene Symbol | GDA |
| Gene ID (NCBI) | 9615 |
| RRID | AB_3670006 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q9Y2T3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GDA ( guanine deaminase), also known as KIAA1258. The protein is enriched in the brain, intestine, kidney and liver. Cytoplasmic expression in the small intestine, hepatocytes, pancreatic islet cells and kidney tubules. This gene encodes an enzyme responsible for the hydrolytic deamination of guanine. Studies in rat ortholog suggest this gene plays a role in microtubule assembly. The molecular weight of GDA is 51 kDa, and it can recognize other isoforms.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GDA antibody 31493-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
