BMP9 Polyclonal antibody

BMP9 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 17769-1-AP

Synonyms

BMP 9, BMP9, BMP-9, Bone morphogenetic protein 9, GDF 2, GDF2

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Validation Data Gallery

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  WB analysis of L02 using 17769-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 17769-1-AP (BMP9 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SMMC-7721 using 17769-1-AP

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 17769-1-AP (BMP9 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 17769-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17769-1-AP (BMP9 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver cancer using 17769-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17769-1-AP (BMP9 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human hepatocirrhosis using 17769-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 17769-1-AP (BMP9 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human hepatocirrhosis using 17769-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 17769-1-AP (BMP9 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 17769-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17769-1-AP (BMP9 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 17769-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17769-1-AP (BMP9 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: L02 cells, SMMC-7721 cells
• Positive IHC detected in: human liver cancer tissue, human hepatocirrhosis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 17769-1-AP is BMP9 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BMP9 fusion protein Ag12177
• Full Name: growth differentiation factor 2
• Calculated Molecular Weight: 429 aa, 47 kDa
• Observed Molecular Weight: 30 kDa, 46 kDa
• GenBank Accession Number: BC074921
• Gene Symbol: GDF2
• Gene ID (NCBI): 2658
• RRID: AB_2878437
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BMP9 is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Studies in rodents suggest that this protein plays a role in the adult liver and in differentiation of cholinergic central nervous system neurons. Mutations in this gene are associated with hereditary hemorrhagic telangiectasia. Human BMP9 precursor protein contains 429 amino acids and has a calculated molecular mass of about 47 kD. Posttranslational processing results in a mature BMP9 protein of about 13 kD. Once secreted, BMP9 may exist as dimers of mature regions or as BMP9 proregion complexes in which the proregion of BMP9 remains tightly associated with the mature dimer.