GFAP Recombinant antibody

GFAP Recombinant Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


Human, mouse, rat , pig







Cat no : 81063-1-RR


FLJ45472, GFAP

Tested Applications

Positive WB detected inU-251 cells, rat brain tissue, mouse brain tissue, pig brain tissue
Positive IHC detected inrat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inrat brain tissue, mouse brain tissue

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:4950-1:19800
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

81063-1-RR targets GFAP in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat , pig samples.

Tested Reactivity Human, mouse, rat , pig
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GFAP fusion protein Ag10423
Full Name glial fibrillary acidic protein
Calculated Molecular Weight 432 aa, 50 kDa
GenBank Accession NumberBC013596
Gene Symbol GFAP
Gene ID (NCBI) 2670
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GFAP (Glial fibrillary acidic protein ), an intermediate-filament (IF) protein , is specifically expressed in cells of astroglial lineage and is widely used to mark astroglia in the brain. It is also used as a marker for intracranial and intraspinal tumors arising from astrocytes.


Product Specific Protocols
WB protocol for GFAP antibody 81063-1-RRDownload protocol
IHC protocol for GFAP antibody 81063-1-RRDownload protocol
IF protocol for GFAP antibody 81063-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols