VISTA Polyclonal antibody

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  WB analysis using 24849-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24849-1-AP (VISTA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 24849-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 24849-1-AP (VISTA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 24849-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 24849-1-AP (VISTA antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 24849-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 24849-1-AP (VISTA antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 24849-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24849-1-AP (VISTA antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 24849-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24849-1-AP (VISTA antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 24849-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24849-1-AP (VISTA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 24849-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24849-1-AP (VISTA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human tonsillitis using 24849-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 24849-1-AP (VISTA rabbit polyclonal antibody) at dilution of 1:100 and 60181-1-Ig (CD3 mouse monoclonal antibody), further stained with CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) for 24849-1-AP and CoraLite594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) for 60181-1-Ig.

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  IF Staining of human placenta using 24849-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using VISTA antibody (24849-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human placenta using 24849-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using VISTA antibody (24849-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human placenta using 24849-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using VISTA antibody (24849-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

VISTA Polyclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 24849-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF-P, ELISA

Synonyms

C10orf54, B7-H5, Sisp-1, SISP1, PP2135

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Tested Applications

• Positive WB detected in: human placenta tissue, SH-SY5Y cells, human peripheral blood platelets
• Positive IHC detected in: human placenta tissue, human colon cancer tissue, human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human placenta tissue, human tonsillitis tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

24849-1-AP targets VISTA in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag20600

  Product name: Recombinant human C10orf54 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 34-196 aa of BC020568

  Sequence: KVATPYSLYVCPEGQNVTLTCRLLGPVDKGHDVTFYKTWYRSSRGEVQTCSERRPIRNLTFQDLHLHHGGHQAANTSHDLAQRHGLESASDHHGNFSITMRNLTLLDSGLYCCLVVEIRHHHSEHRVHGAMELQVQTGKDAPSNCVVYPSSSQESENITAAAL

• Full Name: chromosome 10 open reading frame 54
• Calculated Molecular Weight: 311 aa, 34 kDa
• Observed Molecular Weight: 45-60 kDa
• GenBank Accession Number: BC020568
• Gene Symbol: VISTA
• Gene ID (NCBI): 64115
• RRID: AB_2879755
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H7M9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VISTA, also known as platelet receptor Gi24, B7-H5, C10orf54, Dies1, PD-1H and SISP1, is a member of the immunoglobulin superfamily. It serves as not only as a positive regulator but also as a substrate of MT1-MMP, and contributes at least in part to tumor invasion.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	J Nutr Biochem; Isothiocyanates attenuate immune checkpoint blockage therapy in gastric cancer via induction of PD-L1 expression; Authors - Qi Zhang
human	IF	Front Cell Dev Biol; Identify the Prognostic and Immune Profile of VSIR in the Tumor Microenvironment: A Pan-Cancer Analysis.; Authors - Yuanyuan Liu