Validation Data Gallery
|Positive WB detected in||mouse ovary tissue, HeLa cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human cervix tissue, human ovary tumor tissue, human skin cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
12880-1-AP targets GJB3 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, marmoset|
|Host / Isotype||Rabbit / IgG|
|Immunogen||GJB3 fusion protein Ag3839|
|Full Name||gap junction protein, beta 3, 31kDa|
|Calculated molecular weight||270 aa, 31 kDa|
|Observed molecular weight||31 kDa|
|GenBank accession number||BC012918|
|Gene ID (NCBI)||2707|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
GJB3, also known as Connexin-31 (Cx31), belongs to the connexin family. Connexins are membrane-spanning proteins that assemble to form gap junction channels that facilitate the transfer of ions and small molecules between cells. Mutations in the gene of GJB3 have been described in patients with dominant and recessive hearing impairment and in patients with erythrokeratodermia variabilis (EKV).
|Product Specific Protocols|
|WB protocol for GJB3 antibody 12880-1-AP||Download protocol|
|IHC protocol for GJB3 antibody 12880-1-AP||Download protocol|
|IF protocol for GJB3 antibody 12880-1-AP||Download protocol|
|IP protocol for GJB3 antibody 12880-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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