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GJB3 Polyclonal antibody

GJB3 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (1)





Cat no : 12880-1-AP


Connexin 31, CX31, DFNA2, DFNA2B, EKV, Gap junction beta 3 protein, GJB3

Tested Applications

Positive WB detected inmouse stomach tissue, mouse ovary tissue, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse placenta tissue, human ovary tumor tissue, human cervix tissue, human skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12880-1-AP targets GJB3 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, marmoset
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GJB3 fusion protein Ag3839
Full Name gap junction protein, beta 3, 31kDa
Calculated Molecular Weight 270 aa, 31 kDa
Observed Molecular Weight 31 kDa
GenBank Accession NumberBC012918
Gene Symbol GJB3
Gene ID (NCBI) 2707
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GJB3, also known as Connexin-31 (Cx31), belongs to the connexin family. Connexins are membrane-spanning proteins that assemble to form gap junction channels that facilitate the transfer of ions and small molecules between cells. Mutations in the gene of GJB3 have been described in patients with dominant and recessive hearing impairment and in patients with erythrokeratodermia variabilis (EKV).


Product Specific Protocols
WB protocol for GJB3 antibody 12880-1-APDownload protocol
IHC protocol for GJB3 antibody 12880-1-APDownload protocol
IF protocol for GJB3 antibody 12880-1-APDownload protocol
IP protocol for GJB3 antibody 12880-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Cell Rep

Cochlear Cell Modeling Using Disease-Specific iPSCs Unveils a Degenerative Phenotype and Suggests Treatments for Congenital Progressive Hearing Loss.

Authors - Makoto Hosoya

Stem Cells

FBXL12-Mediated Degradation of ALDH3 Is Essential for Trophoblast Differentiation during Placental Development.

Authors - Masaaki Nishiyama

J Neurosci

Glial-Specific Deletion of Med12 Results in Rapid Hearing Loss via Degradation of the Stria Vascularis.

Authors - Teng-Wei Huang

Sci Rep

Distinct Expression Patterns Of Causative Genes Responsible For Hereditary Progressive Hearing Loss In Non-Human Primate Cochlea.

Authors - Makoto Hosoya


In vitro fertilization causes excessive glycogen accumulation in mouse placenta.

Authors - Jie Dong

J Pain Res

PMID: 29692624

Authors - Brice Bellessort