GLI2-Specific Polyclonal antibody

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  WB analysis using 18989-1-AP

  Mouse brain tissue were subjected to SDS PAGE followed by western blot with 18989-1-AP (GLI2-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 18989-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 18989-1-AP (GLI2-Specific antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 18989-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 18989-1-AP (GLI2-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 18989-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18989-1-AP (GLI2-Specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 18989-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18989-1-AP (GLI2-Specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 18989-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 18989-1-AP (GLI2-Specific antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of MCF-7 using 18989-1-AP

  Immunofluorescent analysis of MCF-7 cells, using Gli2 antibody 18989-1-AP at 1:25 dilution and FITC- labeled FITC-Donkey anti-Rabbit IgG (green).

• Featured Product
• KD/KO Validated

GLI2-Specific Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 18989-1-AP

Publications(56)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Gli2, Thp, Tax helper protein, GLI Kruppel family member GLI2, Gli 2

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Tested Applications

• Positive WB detected in: mouse brain tissue, HEK-293 cells, human testis tissue
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 50 publications below
• IHC: See 9 publications below
• IF: See 10 publications below

Product Information

18989-1-AP targets GLI2-Specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, chamaeleo calyptratus
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: GLI family zinc finger 2
• Calculated Molecular Weight: 168 kDa
• Observed Molecular Weight: 167 kDa， 88 kDa
• GenBank Accession Number: NM_005270
• Gene Symbol: GLI2
• Gene ID (NCBI): 2736
• RRID: AB_10596479
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P10070
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GLI2, also named as THP, belongs to the GLI C2H2-type zinc-finger protein family. GLI2 play a role during embryogenesis. It binds to the DNA sequence 5'-GAACCACCCA-3' which is part of the TRE-2S regulatory element that augments the Tax-dependent enhancer of human T-cell leukemia virus type 1. It is implicated in the transduction of SHH signal. Mutation of GLI2 will cause of holoprosencephaly type 9 (HPE9). Four possible isoforms (hGli2a, b, g, and d) are formed by combinations of two independent alternative splicings, and all the isoforms could bind to a DNA motif, TRE2S. hGli2 a, b, g, and d. These isoforms encode 133-, 131-, 88-, and 86-kDa proteins, respectively.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Cell Death Dis; Wdr4 promotes cerebellar development and locomotion through Arhgap17-mediated Rac1 activation; Authors - Pei-Rung Wu
human	WB	J Cell Biol; Range of SHH signaling in adrenal gland is limited by membrane contact to cells with primary cilia.; Authors - Ivona Mateska
human	WB,IF,IHC	Elife; Transcriptional control of subtype switching ensures adaptation and growth of pancreatic cancer.; Authors - Christina R Adams ; KD Validated
mouse	WB	Acta Pharmacol Sin; 1-Indanone retards cyst development in ADPKD mouse model by stabilizing tubulin and down-regulating anterograde transport of cilia; Authors - Xiao-Wei Li
human	WB	Cell Mol Life Sci; HAS3-induced extracellular vesicles from melanoma cells stimulate IHH mediated c-Myc upregulation via the hedgehog signaling pathway in target cells.; Authors - Uma Thanigai Arasu
human	WB	Front Cell Dev Biol; Apatinib Suppresses Gastric Cancer Stem Cells Properties by Inhibiting the Sonic Hedgehog Pathway.; Authors - Wanshuang Cao