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  • KD/KO Validated

GLI2-Specific Polyclonal antibody

GLI2-Specific Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 18989-1-AP

Synonyms

AW546128, GLI Kruppel family member GLI2, Gli2, GLI2-Specific



Tested Applications

Positive WB detected inmouse brain tissue, HEK-293 cells, human testis tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18989-1-AP targets GLI2-Specific in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, Chamaeleo calyptratus
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name GLI-Kruppel family member GLI2
Calculated Molecular Weight 168 kDa
Observed Molecular Weight167 kDa, 88 kDa
GenBank Accession NumberNM_001081125
Gene Symbol Gli2
Gene ID (NCBI) 14633
RRIDAB_10596479
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GLI2, also named as THP, belongs to the GLI C2H2-type zinc-finger protein family. GLI2 play a role during embryogenesis. It binds to the DNA sequence 5'-GAACCACCCA-3' which is part of the TRE-2S regulatory element that augments the Tax-dependent enhancer of human T-cell leukemia virus type 1. It is implicated in the transduction of SHH signal. Mutation of GLI2 will cause of holoprosencephaly type 9 (HPE9). Four possible isoforms (hGli2a, b, g, and d) are formed by combinations of two independent alternative splicings, and all the isoforms could bind to a DNA motif, TRE2S. hGli2 a, b, g, and d. These isoforms encode 133-, 131-, 88-, and 86-kDa proteins, respectively.

Protocols

Product Specific Protocols
WB protocol for GLI2-Specific antibody 18989-1-APDownload protocol
IHC protocol for GLI2-Specific antibody 18989-1-APDownload protocol
IF protocol for GLI2-Specific antibody 18989-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Death Dis

Wdr4 promotes cerebellar development and locomotion through Arhgap17-mediated Rac1 activation

Authors - Pei-Rung Wu
humanWB

J Cell Biol

Range of SHH signaling in adrenal gland is limited by membrane contact to cells with primary cilia.

Authors - Ivona Mateska
humanWB,IF,IHC

Elife

Transcriptional control of subtype switching ensures adaptation and growth of pancreatic cancer.

Authors - Christina R Adams
  • KD Validated
mouseWB

Acta Pharmacol Sin

1-Indanone retards cyst development in ADPKD mouse model by stabilizing tubulin and down-regulating anterograde transport of cilia

Authors - Xiao-Wei Li
humanWB

Cell Mol Life Sci

HAS3-induced extracellular vesicles from melanoma cells stimulate IHH mediated c-Myc upregulation via the hedgehog signaling pathway in target cells.

Authors - Uma Thanigai Arasu
human,mouseWB

Front Cell Dev Biol

Apatinib Suppresses Gastric Cancer Stem Cells Properties by Inhibiting the Sonic Hedgehog Pathway.

Authors - Wanshuang Cao