GLI3 Polyclonal antibody

GLI3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 28272-1-AP

Synonyms

GLI3-190, GLI3 full-length protein, GLI3 form of 190 kDa, GLI family zinc finger 3, GCPS

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Validation Data Gallery

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  WB analysis of HepG2 using 28272-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 28272-1-AP (GLI3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of SKOV-3 using 28272-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 28272-1-AP (GLI3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 28272-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28272-1-AP (GLI3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 28272-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28272-1-AP (GLI3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 28272-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28272-1-AP (GLI3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 28272-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28272-1-AP (GLI3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 28272-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28272-1-AP (GLI3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 28272-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28272-1-AP (GLI3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 28272-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 28272-1-AP (GLI3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 28272-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 28272-1-AP (GLI3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HepG2 cells, SKOV-3 cells
• Positive IHC detected in: mouse testis tissue, human colon cancer tissue, human prostate cancer tissue, rat testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

28272-1-AP targets GLI3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GLI3 fusion protein Ag28472
• Full Name: GLI family zinc finger 3
• Calculated Molecular Weight: 170 aa
• Observed Molecular Weight: 170-190 kDa, 80-90 kDa
• GenBank Accession Number: NM_000168
• Gene Symbol: GLI3
• Gene ID (NCBI): 2737
• RRID: AB_2881101
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GLI3 belongs to the GLI C2H2-type zinc-finger protein family. GLI3 plays a role in limb and brain development. GLI3 is implicated in the transduction of SHH signal. Defects in GLI3 are the cause of Greig cephalo-poly-syndactyly syndrome (GCPS). Defects in GLI3 are a cause of Pallister-Hall syndrome (PHS). Defects in GLI3 are a cause of type A1/B postaxial polydactyly (PAPA1/PAPB). Defects in GLI3 are a cause of type IV preaxial polydactyly. Defects in GLI3 are the cause of acrocallosal syndrome (ACS). The antibody is specific to GLI3. At the molecular level, Gli3 is translated into a 190-kDa transcriptional activator (Gli3-190) that undergoes proteolytic processing into a truncated 83-kDa repressor (Gli3-83) lacking C-terminal activation domains. (PMID: 16705181)

Publications

Species	Application	Title
rat	WB	Bioengineered; All-trans-retinoic acid suppresses rat embryo hindlimb bud mesenchymal chondrogenesis by modulating HoxD9 expression; Authors - Quan Hong
human	WB	Medicine (Baltimore); miR-200c targeting GLI3 inhibits cell proliferation and promotes apoptosis in non-small cell lung cancer cells; Authors - Xiangjun Yi