GLRB Polyclonal antibody

GLRB Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 15371-1-AP

Synonyms

GLRB, Glycine receptor subunit beta, glycine receptor, beta



Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue, mouse spinal cord tissue
Positive IHC detected inmouse cerebellum tissue, rat cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

The immunogen of 15371-1-AP is GLRB Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GLRB fusion protein Ag4429
Full Name glycine receptor, beta
Calculated molecular weight 56 kDa
Observed molecular weight 56 kDa
GenBank accession numberBC032635
Gene symbol GLRB
Gene ID (NCBI) 2743
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Glycine is an inhibitory neurotransmitter in the central nervous system. The glycine receptor (GlyR) is a member of the nicotinic acetylcholine receptor of ligand-gated ion channels. This receptor has important roles in a variety of physiological processes, especially in mediating inhibitory neurotransmission in the spinal cord and brain stem. GlyR is a pentameric receptor comprised of alpha and beta subunits. Four alpha-subunits (GLRA1, GLRA2, GLRA3, GLRA4) and one beta-subunit (GLRB) of GlyR have been identified. (PMID: 11409700; 15383648)

Protocols

Product Specific Protocols
WB protocol for GLRB antibody 15371-1-APDownload protocol
IHC protocol for GLRB antibody 15371-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

PLoS Biol

mGluR5/ERK signaling regulated the phosphorylation and function of glycine receptor α1ins subunit in spinal dorsal horn of mice.

Authors - Zi-Yang Zhang
mouseWB

Psychopharmacology (Berl)

Contribution of skeletal muscular glycine to rapid antidepressant effects of ketamine in an inflammation-induced mouse model of depression.

Authors - Niannian Huang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

A (Verified Customer) (11-11-2019)

Immunofluorescent analysis of (4% PFA) fixed rat DRG slices using GlyRB rabbit polyclonal antibody at 1:1000 dilution and Alexa 555 at 1:1000 dilution.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Rat DRG
GLRB Antibody Immunofluorescence, validation (1:1000 dilution) in Rat DRG (Cat no:15371-1-AP)