Tested Applications
| Positive WB detected in | A549 cells, Jurkat cells, MCF7 cells, mouse testis tissue, rat testis tissue |
| Positive IHC detected in | human stomach cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11254-1-AP targets GLRX3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1775 Product name: Recombinant human GLRX3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-289 aa of BC014372 Sequence: AQMNEVMAELAKELPQVSFVKLEAEGVPEVSEKYEISSVPTFLFFKNSQKIDRLDGAHAPELTKKVQRHASSGSFLSSANEHLKEDLNLRLKKLTHAAPCMLFMKGTPQEPRCGFSKQMVEILHKHNIQFSSFDIFSDEEVRQGLKAYSSWPTYPQLYVSGELIGGLDIIKELEASEELDTICPKAPKLEERLKVLTNKASVMLFMKGNKQEAKCGFSKQILEILNSTGVEYETFDILEDEEVRQGLKAYSNWPTYPQLYVKGELVGGLDIVKELKENGELLPILRGEN Predict reactive species |
| Full Name | glutaredoxin 3 |
| Calculated Molecular Weight | 37 kDa |
| Observed Molecular Weight | 37-40 kDa |
| GenBank Accession Number | BC014372 |
| Gene Symbol | GLRX3 |
| Gene ID (NCBI) | 10539 |
| RRID | AB_2110374 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O76003 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GLRX3(Glutaredoxin-3) is also named as PICOT, TXNL2. Mammalian glutaredoxin 3 is an essential protein involved in the regulation of signal transduction, for instance during immune cell activation and development of cardiac hypertrophy, presumably in response to redox signals(PMID:20226171). Glrx3 could take a pivotal role in colon and lung cancer cells during the tumorigenesis, which is expressed in lung and colon cancers consistently and preferentially(PMID:19797004).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for GLRX3 antibody 11254-1-AP | Download protocol |
| WB protocol for GLRX3 antibody 11254-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GLRX3 Polyclonal antibody (Cat# 11254-1-AP) has 9 citations published in journals including J Clin Invest, Cell Death Differ, Chest, Haematologica, Toxicology, iScience, Exp Cell Res, Hum Mol Genet, and Biosci Biotechnol Biochem. Research fields span iron-sulfur cluster biogenesis, frataxin deficiency, neuromuscular disorders, COPD proteomics, heme biosynthesis, mitochondrial damage/ferroptosis, iron homeostasis, oxidative stress, and neuroprotection. Applications cited include WB and IHC in human and mouse samples.
| Species | Application | Title |
|---|---|---|
J Clin Invest CIAO1 loss of function causes a neuromuscular disorder with compromise of nucleocytoplasmic Fe-S enzymes | ||
Cell Death Differ Partial bypass of frataxin deficiency by ISCU M141I restores cytosolic and nuclear Fe-S cluster assembly. | ||
Haematologica Dimeric ferrochelatase bridges ABCB7 and ABCB10 homodimers in an architecturally defined molecular complex required for heme biosynthesis. | ||
Toxicology Combined exposure to polystyrene nanoplastics and bisphenol A results in mitochondrial damage and ferroptosis via the PI3K-AKT signaling pathway in mice kidneys. | ||
iScience Pathological frataxin deficiency in mice causes tissue-specific alterations in iron homeostasis. |
