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- KD/KO Validated
KGA/GAC Polyclonal antibody
KGA/GAC Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat and More (1)
WB, IP, IHC, IF, CoIP, ELISA
Cat no : 12855-1-AP
Validation Data Gallery
|Positive WB detected in||mouse brain tissue, COLO 320 cells, mouse kidney tissue, HEK-293 cells, rat liver tissue, rat brain tissue|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human gliomas tissue, human kidney tissue, human brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HEK-293 cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:2000-1:5000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 10 publications below|
|WB||See 71 publications below|
|IHC||See 4 publications below|
|IF||See 12 publications below|
|IP||See 2 publications below|
|CoIP||See 1 publications below|
12855-1-AP targets KGA/GAC in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, cat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||KGA/GAC fusion protein Ag3505|
|Calculated molecular weight||669 aa, 73 kDa|
|Observed molecular weight||58 kDa, 65 kDa|
|GenBank accession number||BC038507|
|Gene ID (NCBI)||2744|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
GLS, also named as GLS1 and KIAA0838, belongs to the glutaminase family. It catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Glutaminase-, glutamate-, and taurine-immunoreactive neurons develop neurofibrillary tangles in Alzheimer's disease(PMID:1672808).The glutaminase band in AA/C1 cells is more intense than in HT29 cells, in accordance with measurements of glutaminase activity, and had the same molecular mass of approx. 63 kDa. The bands for both cell lines are clearly different in size from both rat liver glutaminase (58 kDa) and rat kidney glutaminase (65 kDa)(PMID:12408749). It also reveals a molecular weight of 83-84 kDa as a phosphate-dependent glutaminase(PMID:447624;7512428). It has 3 isoforms named as KGA, GAM, GAC. This antibody can recognize KGA and GAC.
|Product Specific Protocols|
|WB protocol for KGA/GAC antibody 12855-1-AP||Download protocol|
|IHC protocol for KGA/GAC antibody 12855-1-AP||Download protocol|
|IF protocol for KGA/GAC antibody 12855-1-AP||Download protocol|
|IP protocol for KGA/GAC antibody 12855-1-AP||Download protocol|
|Click here to view our Standard Protocols|
CFIm25 links alternative polyadenylation to glioblastoma tumour suppression.
Glia-to-Neuron Conversion by CRISPR-CasRx Alleviates Symptoms of Neurological Disease in Mice.
Systemic elevation of PTEN induces a tumor-suppressive metabolic state.
Rewiring of Glutamine Metabolism Is a Bioenergetic Adaptation of Human Cells with Mitochondrial DNA Mutations.
In Colorectal Cancer Cells With Mutant KRAS, SLC25A22-Mediated Glutaminolysis Reduces DNA Demethylation to Increase WNT Signaling, Stemness, and Drug Resistance.
Inhibition of Glutamine Utilization Synergizes with Immune Checkpoint Inhibitor to Promote Antitumor Immunity.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Kishor (Verified Customer) (01-13-2020)
Anti body works for human and mouse proteins. 1:1000 dilution in BSA+TBST is good.