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  • KD/KO Validated

KGA/GAC Polyclonal antibody

KGA/GAC Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (1)





Cat no : 12855-1-AP


AAD20, GAC, GLS, GLS1, glutaminase, K glutaminase, KGA, KGA/GAC, KIAA0838, L glutamine amidohydrolase

Tested Applications

Positive WB detected inmouse brain tissue, COLO 320 cells, mouse kidney tissue, HEK-293 cells, rat liver tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman gliomas tissue, human kidney tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells, L02 cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12855-1-AP targets KGA/GAC in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, cat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KGA/GAC fusion protein Ag3505
Full Name glutaminase
Calculated Molecular Weight 669 aa, 73 kDa
Observed Molecular Weight58 kDa, 65 kDa
GenBank Accession NumberBC038507
Gene Symbol GLS
Gene ID (NCBI) 2744
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GLS, also named as GLS1 and KIAA0838, belongs to the glutaminase family. It catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Glutaminase-, glutamate-, and taurine-immunoreactive neurons develop neurofibrillary tangles in Alzheimer's disease(PMID:1672808).The glutaminase band in AA/C1 cells is more intense than in HT29 cells, in accordance with measurements of glutaminase activity, and had the same molecular mass of approx. 63 kDa. The bands for both cell lines are clearly different in size from both rat liver glutaminase (58 kDa) and rat kidney glutaminase (65 kDa)(PMID:12408749). It also reveals a molecular weight of 83-84 kDa as a phosphate-dependent glutaminase(PMID:447624;7512428). It has 3 isoforms named as KGA, GAM, GAC. This antibody can recognize KGA and GAC.


Product Specific Protocols
WB protocol for KGA/GAC antibody 12855-1-APDownload protocol
IHC protocol for KGA/GAC antibody 12855-1-APDownload protocol
IF protocol for KGA/GAC antibody 12855-1-APDownload protocol
IP protocol for KGA/GAC antibody 12855-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




CFIm25 links alternative polyadenylation to glioblastoma tumour suppression.

Authors - Chioniso P Masamha


Glia-to-Neuron Conversion by CRISPR-CasRx Alleviates Symptoms of Neurological Disease in Mice.

Authors - Haibo Zhou


Systemic elevation of PTEN induces a tumor-suppressive metabolic state.

Authors - Garcia-Cao Isabel I

Cell Metab

Rewiring of Glutamine Metabolism Is a Bioenergetic Adaptation of Human Cells with Mitochondrial DNA Mutations.

Authors - Qiuying Chen

Nat Metab

Quantitative analysis of metabolic fluxes in brown fat and skeletal muscle during thermogenesis

Authors - Grace Park

Brain Behav Immun

Modulation of immune function by glutamatergic neurons in the cerebellar interposed nucleus via hypothalamic and sympathetic pathways.

Authors - Fen-Fen Xu


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Kishor (Verified Customer) (01-13-2020)

Anti body works for human and mouse proteins. 1:1000 dilution in BSA+TBST is good.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: H69, HUCCT-1 and Rat Liver tissue
KGA/GAC Antibody Western Blot, validation (1:1000 dilution) in H69, HUCCT-1 and Rat Liver tissue (Cat no:12855-1-AP)