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- KD/KO Validated
KGA/GAC Polyclonal antibody
KGA/GAC Polyclonal Antibody for IF, IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IP, IHC, IF,ELISA
Conjugate
Unconjugated
Cat no : 12855-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, COLO 320 cells, mouse brain tissue, mouse kidney tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human gliomas tissue, human kidney tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:2000-1:5000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 9 publications below |
| WB | See 47 publications below |
| IHC | See 3 publications below |
| IF | See 9 publications below |
| IP | See 1 publications below |
Product Information
12855-1-AP targets KGA/GAC in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | cat, human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KGA/GAC fusion protein Ag3505 |
| Full Name | glutaminase |
| Calculated molecular weight | 669 aa, 73 kDa |
| Observed molecular weight | 65 kDa, 58 kDa |
| GenBank accession number | BC038507 |
| Gene symbol | GLS |
| Gene ID (NCBI) | 2744 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GLS, also named as GLS1 and KIAA0838, belongs to the glutaminase family. It catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Glutaminase-, glutamate-, and taurine-immunoreactive neurons develop neurofibrillary tangles in Alzheimer's disease(PMID:1672808).The glutaminase band in AA/C1 cells is more intense than in HT29 cells, in accordance with measurements of glutaminase activity, and had the same molecular mass of approx. 63 kDa. The bands for both cell lines are clearly different in size from both rat liver glutaminase (58 kDa) and rat kidney glutaminase (65 kDa)(PMID:12408749). It also reveals a molecular weight of 83-84 kDa as a phosphate-dependent glutaminase(PMID:447624;7512428). It has 3 isoforms named as KGA, GAM, GAC. This antibody can recognize KGA and GAC.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for KGA/GAC antibody 12855-1-AP | Download protocol |
| IHC protocol for KGA/GAC antibody 12855-1-AP | Download protocol |
| IF protocol for KGA/GAC antibody 12855-1-AP | Download protocol |
| IP protocol for KGA/GAC antibody 12855-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Am J Respir Cell Mol Biol Inhibition of Glutaminase 1 Attenuates Experimental Pulmonary Fibrosis.
| ||
Am J Respir Cell Mol Biol Glutamine Metabolism is Required for Collagen Protein Synthesis in Lung Fibroblasts.
| ||
Exp Cell Res GLS1 promotes proliferation in hepatocellular carcinoma cells via AKT/GSK3β/CyclinD1 pathway.
| ||
Oncotarget Myc-induced glutaminolysis bypasses HIF-driven glycolysis in hypoxic small cell lung carcinoma cells. | ||
Am J Respir Cell Mol Biol Glutaminolysis Promotes Collagen Translation and Stability via α-ketoglutarate Mediated mTOR Activation and Proline Hydroxylation.
| ||
Am J Respir Cell Mol Biol Glutaminolysis Epigenetically Regulates Anti-Apoptotic Gene Expression in IPF Fibroblasts.
|
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Kishor (Verified Customer) (01-13-2020) | Anti body works for human and mouse proteins. 1:1000 dilution in BSA+TBST is good.
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