KGA/GAC Polyclonal antibody

• Featured Product
• KD/KO Validated

KGA/GAC Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

79

Reviews (1)

Cat no : 12855-1-AP

Synonyms

AAD20, GAC, GLS, GLS1, glutaminase, K glutaminase, KGA, KGA/GAC, KIAA0838, L glutamine amidohydrolase

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Validation Data Gallery

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  WB analysis of HEK-293 using 12855-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12855-1-AP (KGA/GAC antibody at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  WB analysis using 12855-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12855-1-AP (KGA/GAC antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12855-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12855-1-AP (KGA/GAC antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12855-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12855-1-AP (KGA/GAC antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12855-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12855-1-AP (KGA/GAC antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 12855-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12855-1-AP (KGA/GAC antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12855-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12855-1-AP (KGA/GAC antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 12855-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 12855-1-AP (KGA/GAC antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 12855-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12855-1-AP (KGA/GAC antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 12855-1-AP

  IP result of anti-KGA/GAC (IP:12855-1-AP, 4ug; Detection:12855-1-AP 1:5000) with mouse brain tissue lysate 2400 ug.

• 

  IP experiment of mouse brain using 12855-1-AP

  IP result of anti-KGA/GAC (IP:12855-1-AP, 4ug; Detection:12855-1-AP 1:5000) with mouse brain tissue lysate 2400 ug.

• 

  IHC staining of human gliomas using 12855-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12855-1-AP (KGA/GAC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 12855-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12855-1-AP (KGA/GAC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 12855-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 12855-1-AP (KGA/GAC antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 12855-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 12855-1-AP (KGA/GAC antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 12855-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 12855-1-AP (KGA/GAC antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 12855-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 12855-1-AP (KGA/GAC antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human gliomas using 12855-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12855-1-AP (KGA/GAC antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 12855-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12855-1-AP (KGA/GAC antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 12855-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 12855-1-AP (KGA/GAC antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse brain tissue, COLO 320 cells, mouse kidney tissue, HEK-293 cells, rat liver tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human gliomas tissue, human kidney tissue, human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:2000-1:5000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 10 publications below
• WB: See 71 publications below
• IHC: See 4 publications below
• IF: See 12 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Product Information

12855-1-AP targets KGA/GAC in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, cat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KGA/GAC fusion protein Ag3505
• Full Name: glutaminase
• Calculated molecular weight: 669 aa, 73 kDa
• Observed molecular weight: 58 kDa, 65 kDa
• GenBank accession number: BC038507
• Gene symbol: GLS
• Gene ID (NCBI): 2744
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GLS, also named as GLS1 and KIAA0838, belongs to the glutaminase family. It catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Glutaminase-, glutamate-, and taurine-immunoreactive neurons develop neurofibrillary tangles in Alzheimer's disease(PMID:1672808).The glutaminase band in AA/C1 cells is more intense than in HT29 cells, in accordance with measurements of glutaminase activity, and had the same molecular mass of approx. 63 kDa. The bands for both cell lines are clearly different in size from both rat liver glutaminase (58 kDa) and rat kidney glutaminase (65 kDa)(PMID:12408749). It also reveals a molecular weight of 83-84 kDa as a phosphate-dependent glutaminase(PMID:447624;7512428). It has 3 isoforms named as KGA, GAM, GAC. This antibody can recognize KGA and GAC.

Publications

Species	Application	Title
human	WB	Nature; CFIm25 links alternative polyadenylation to glioblastoma tumour suppression.; Authors - Chioniso P Masamha
mouse	IF	Cell; Glia-to-Neuron Conversion by CRISPR-CasRx Alleviates Symptoms of Neurological Disease in Mice.; Authors - Haibo Zhou
mouse	WB	Cell; Systemic elevation of PTEN induces a tumor-suppressive metabolic state.; Authors - Garcia-Cao Isabel I
	WB	Cell Metab; Rewiring of Glutamine Metabolism Is a Bioenergetic Adaptation of Human Cells with Mitochondrial DNA Mutations.; Authors - Qiuying Chen
human	WB	Gastroenterology; In Colorectal Cancer Cells With Mutant KRAS, SLC25A22-Mediated Glutaminolysis Reduces DNA Demethylation to Increase WNT Signaling, Stemness, and Drug Resistance.; Authors - Chi Chun Wong
human,mouse	WB	Mol Cell; Inhibition of Glutamine Utilization Synergizes with Immune Checkpoint Inhibitor to Promote Antitumor Immunity.; Authors - Jun-Kyu Byun ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Kishor (Verified Customer) (01-13-2020)

Anti body works for human and mouse proteins. 1:1000 dilution in BSA+TBST is good.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: H69, HUCCT-1 and Rat Liver tissue

KGA/GAC Antibody Western Blot, validation (1:1000 dilution) in H69, HUCCT-1 and Rat Liver tissue (Cat no:12855-1-AP)

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