KGA/GAM/GAC Polyclonal antibody

• Featured Product
• KD/KO Validated

KGA/GAM/GAC Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 23549-1-AP

Synonyms

AAD20, GAC, GAM, GLS, GLS1, glutaminase, K glutaminase, KGA, KGA/GAM/GAC, KIAA0838, L glutamine amidohydrolase

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Validation Data Gallery

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  WB analysis of HEK-293 using 23549-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23549-1-AP (KGA/GAM/GAC antibody at dilution of 1:600 incubated at 4 degree celsius over night.

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  WB analysis of HeLa using 23549-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23549-1-AP (KGA/GAM/GAC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 23549-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23549-1-AP (KGA/GAM/GAC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 23549-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 23549-1-AP (KGA/GAM/GAC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 23549-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 23549-1-AP (KGA/GAM/GAC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 23549-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 23549-1-AP (KGA/GAM/GAC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 23549-1-AP

  IP Result of anti-KGA/GAM/GAC (IP:23549-1-AP, 4ug; Detection:23549-1-AP 1:1000) with HEK-293 cells lysate 1200ug.

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  IHC staining of human kidney using 23549-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 23549-1-AP (KGA/GAM/GAC antibody at dilution of 1:50.

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  IHC staining of human kidney using 23549-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 23549-1-AP (KGA/GAM/GAC antibody at dilution of 1:50.

• 

  IHC staining of human brain using 23549-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain slide using 23549-1-AP (KGA/GAM/GAC antibody at dilution of 1:50.

• 

  IHC staining of human brain using 23549-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain slide using 23549-1-AP (KGA/GAM/GAC antibody at dilution of 1:50.

• 

  IF Staining of HEK-293 using 23549-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 23549-1-AP (KGA/GAM/GAC antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HEK-293 using 23549-1-AP

  Immunofluorescent analysis of HEK-293 cells using 23549-1-AP (KGA/GAM/GAC antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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  IF Staining of HeLa using 23549-1-AP

  Immunofluorescent analysis of HeLa cells using 23549-1-AP (KGA/GAM/GAC antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, mouse brain tissue, mouse liver tissue, rat brain tissue
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human kidney tissue, human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HEK-293 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:25-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below

Product Information

The immunogen of 23549-1-AP is KGA/GAM/GAC Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KGA/GAM/GAC fusion protein Ag20354
• Full Name: glutaminase
• Calculated molecular weight: 669 aa, 73 kDa
• Observed molecular weight: 58 kDa, 65 kDa, 83 kDa
• GenBank accession number: BC038507
• Gene symbol: GLS
• Gene ID (NCBI): 2744
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GLS, also named as GLS1 and KIAA0838, belongs to the glutaminase family. It catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Glutaminase-, glutamate-, and taurine-immunoreactive neurons develop neurofibrillary tangles in Alzheimer's disease.The glutaminase band in AA/C1 cells is more intense than in HT29 cells, in accordance with measurements of glutaminase activity, and had the same molecular mass of approx. 63 kDa(PMID:12408749). Ping Gao et al. (2009) determined that mitochondrial glutaminase expression (GLS, molecular mass of ~58 kDa) is increased ~10-fold in response to Myc. It also reveals a molecular weight of 83-84 kDa as a phosphate-dependent glutaminase(PMID:447624;7512428). GLS has 3 isoforms produced by alternative splicing and this antibody can recognize all the 3 isoforms(KGA,GAM,GAC) of GLS.

Publications

Species	Application	Title
human	WB,IF	Mol Cell; Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.; Authors - Bin Jiang ; KO Validated
human	WB	Redox Biol; Mitochondrial thiol modification by a targeted electrophile inhibits metabolism in breast adenocarcinoma cells by inhibiting enzyme activity and protein levels.; Authors - M Ryan Smith