Validation Data Gallery
|Positive WB detected in||HEK-293 cells, HeLa cells, mouse brain tissue, mouse liver tissue, rat brain tissue|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human kidney tissue, human brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HEK-293 cells, HeLa cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:25-1:200|
|Sample-dependent, check data in validation data gallery|
The immunogen of 23549-1-AP is KGA/GAM/GAC Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||KGA/GAM/GAC fusion protein Ag20354|
|Calculated molecular weight||669 aa, 73 kDa|
|Observed molecular weight||58 kDa, 65 kDa, 83 kDa|
|GenBank accession number||BC038507|
|Gene ID (NCBI)||2744|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
GLS, also named as GLS1 and KIAA0838, belongs to the glutaminase family. It catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Glutaminase-, glutamate-, and taurine-immunoreactive neurons develop neurofibrillary tangles in Alzheimer's disease.The glutaminase band in AA/C1 cells is more intense than in HT29 cells, in accordance with measurements of glutaminase activity, and had the same molecular mass of approx. 63 kDa(PMID:12408749). Ping Gao et al. (2009) determined that mitochondrial glutaminase expression (GLS, molecular mass of ~58 kDa) is increased ~10-fold in response to Myc. It also reveals a molecular weight of 83-84 kDa as a phosphate-dependent glutaminase(PMID:447624;7512428). GLS has 3 isoforms produced by alternative splicing and this antibody can recognize all the 3 isoforms(KGA,GAM,GAC) of GLS.
|Product Specific Protocols|
|WB protocol for KGA/GAM/GAC antibody 23549-1-AP||Download protocol|
|IHC protocol for KGA/GAM/GAC antibody 23549-1-AP||Download protocol|
|IF protocol for KGA/GAM/GAC antibody 23549-1-AP||Download protocol|
|IP protocol for KGA/GAM/GAC antibody 23549-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Mitochondrial thiol modification by a targeted electrophile inhibits metabolism in breast adenocarcinoma cells by inhibiting enzyme activity and protein levels.
Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.