Validation Data Gallery
|Positive WB detected in||rat heart tissue, human heart tissue, C2C12 cells, human skeletal muscle tissue, rat skeletal muscle tissue, mouse heart tissue, mouse skeletal muscle tissue, mouse adipose tissue|
|Positive IHC detected in||mouse heart tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||mouse skeletal muscle tissue|
|Western Blot (WB)||WB : 1:1000-1:3000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
66846-1-Ig targets GLUT4 in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.
|Tested Reactivity||Human, mouse, rat, pig|
|Cited Reactivity||human, mouse, rat, hamster|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||GLUT4 fusion protein Ag15390|
|Full Name||solute carrier family 2 (facilitated glucose transporter), member 4|
|Calculated molecular weight||509 aa, 55 kDa|
|Observed molecular weight||48-50 kDa|
|GenBank accession number||BC069615|
|Gene ID (NCBI)||6517|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Glucose transporter 4 (GLUT4), also known as solute carrier family 2, facilitated glucose transporter member 4 (SLC2A4), is a transporter protein regulating glucose transport across cell membranes in an INS-dependent manner.
What is the molecular weight of GLUT4? Is GLUT4 post-translationally modified?
The molecular weight of GLUT4 transporter is 55 kDa. GLUT4 can be N-glycosylated, which is important for its stability and trafficking between the recycling compartment and plasma membrane (PMID: 21757715 and 22545627), and it can also be ubiquitinated and phosphorylated (PMID: 23665900).
What is the subcellular localization of GLUT4?
Glucose transporters, including GLUT4, are multiple-pass integral membrane proteins. GLUT4 is present at the plasma membrane but is also a subject of recycling between plasma membrane and endosomes. The localization of GLUT4 depends on stimulation with INS - in basal conditions GLUT4 is retained intracellularly, while upon INS stimulation it is translocated to the plasma membrane (PMID: 18570632).
What molecules can be transported by GLUT4?
Although the main substrate of GLUT4 transport is glucose, it can also transport glucosamine.
What is the tissue expression pattern of GLUT4?
GLUT4 is expressed in white and brown adipose tissue and in muscle and heart cells, where it is the main glucose transporter responsible for peripheral glucose uptake in response to INS.
Which cell lines can be used to study insulin-dependent GLUT4 protein translocation in glucose uptake assays?
Fat and muscle cells are primarily used in glucose uptake assays because they physiologically respond to INS(PMID: 26646194). The most commonly used cell lines are 3T3-L1 cells (murine pre-adipose fibroblasts), L6 cells (rat myoblasts), and C2C12 cells (murine myoblasts).
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