GLUT4 Monoclonal antibody

GLUT4 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat, pig and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

29

CloneNo.

3G7C9

Cat no : 66846-1-Ig

Synonyms

GLUT 4, GLUT4, SLC2A4

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Validation Data Gallery

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  WB analysis using 66846-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66846-1-Ig (GLUT4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 66846-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66846-1-Ig (GLUT4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 66846-1-Ig

  human heart tissue were subjected to SDS PAGE followed by western blot with 66846-1-Ig (GLUT4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human skeletal muscle using 66846-1-Ig

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66846-1-Ig (GLUT4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse heart using 66846-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66846-1-Ig (GLUT4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 66846-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66846-1-Ig (GLUT4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse skeletal muscle using 66846-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using GLUT4 antibody (66846-1-Ig, Clone: 3G7C9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse skeletal muscle using 66846-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using GLUT4 antibody (66846-1-Ig, Clone: 3G7C9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: rat heart tissue, human heart tissue, human skeletal muscle tissue, C2C12 cells
• Positive IHC detected in: mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: mouse skeletal muscle tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 26 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Product Information

66846-1-Ig targets GLUT4 in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

• Tested Reactivity: Human, mouse, rat, pig
• Cited Reactivity: human, mouse, rat, hamster
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: GLUT4 fusion protein Ag15390
• Full Name: solute carrier family 2 (facilitated glucose transporter), member 4
• Calculated molecular weight: 509 aa, 55 kDa
• Observed molecular weight: 48-50 kDa
• GenBank accession number: BC069615
• Gene symbol: SLC2A4
• Gene ID (NCBI): 6517
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Glucose transporter 4 (GLUT4), also known as solute carrier family 2, facilitated glucose transporter member 4 (SLC2A4), is a transporter protein regulating glucose transport across cell membranes in an INS-dependent manner.

What is the molecular weight of GLUT4? Is GLUT4 post-translationally modified?

The molecular weight of GLUT4 transporter is 55 kDa. GLUT4 can be N-glycosylated, which is important for its stability and trafficking between the recycling compartment and plasma membrane (PMID: 21757715 and 22545627), and it can also be ubiquitinated and phosphorylated (PMID: 23665900).

What is the subcellular localization of GLUT4?

Glucose transporters, including GLUT4, are multiple-pass integral membrane proteins. GLUT4 is present at the plasma membrane but is also a subject of recycling between plasma membrane and endosomes. The localization of GLUT4 depends on stimulation with  INS - in basal conditions GLUT4 is retained intracellularly, while upon  INS stimulation it is translocated to the plasma membrane (PMID: 18570632).

What molecules can be transported by GLUT4?

Although the main substrate of GLUT4 transport is glucose, it can also transport glucosamine.

What is the tissue expression pattern of GLUT4?

GLUT4 is expressed in white and brown adipose tissue and in muscle and heart cells, where it is the main glucose transporter responsible for peripheral glucose uptake in response to INS.

Which cell lines can be used to study insulin-dependent GLUT4 protein translocation in glucose uptake assays?

Fat and muscle cells are primarily used in glucose uptake assays because they physiologically respond to  INS(PMID: 26646194). The most commonly used cell lines are 3T3-L1 cells (murine pre-adipose fibroblasts), L6 cells (rat myoblasts), and C2C12 cells (murine myoblasts).

Publications

Species	Application	Title
mouse	IHC	Aging (Albany NY); High-fat diet induced discrepant peripheral and central nervous systems insulin resistance in APPswe/PS1dE9 and wild-type C57BL/6J mice.; Authors - Yujie Guo
human	WB	Cell Death Dis; Integrated analysis of long non-coding RNAs and mRNAs associated with malignant transformation of gastrointestinal stromal tumors.; Authors - Xiaonan Yin
mouse	WB	Oxid Med Cell Longev; Protective Effect of Jiang Tang Xiao Ke Granules against Skeletal Muscle IR via Activation of the AMPK/SIRT1/PGC-1α Signaling Pathway.; Authors - Ying Bai
mouse	WB	Physiol Rep; Effects of endurance training on the expression of host proteins involved in SARS-CoV-2 cell entry in C57BL/6J mouse.; Authors - Yuki Tamura
mouse	WB	Oxid Med Cell Longev; Total Sesquiterpene Glycosides from Loquat Leaves Ameliorate HFD-Induced Insulin Resistance by Modulating IRS-1/GLUT4, TRPV1, and SIRT6/Nrf2 Signaling Pathways.; Authors - Ruoyun Wu
rat	WB	Acta Diabetol; Adrenomedullin ameliorates palmitic acid-induced insulin resistance through PI3K/Akt pathway in adipocytes.; Authors - Hang-Bing Dai