GMPPB Polyclonal antibody

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• GMPPB Polyclonal antibody (15094-1-AP)

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GMPPB Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 15094-1-AP Copied!

Publications (9)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Mannose-1-phosphate guanyltransferase beta, KIAA1851, GTP-mannose-1-phosphate guanylyltransferase beta, GDP-mannose pyrophosphorylase B, EC:2.7.7.13

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  WB analysis using 15094-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15094-1-AP (GMPPB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse brain using 15094-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15094-1-AP (GMPPB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human ovary cancer using 15094-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 15094-1-AP (GMPPB antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HEK-293 using 15094-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 15094-1-AP (GMPPB antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue, mouse brain tissue, rat skeletal muscle tissue
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15094-1-AP targets GMPPB in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7168

  Product name: Recombinant human GMPPB protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-360 aa of BC001141

  Sequence: MKALILVGGYGTRLRPLTLSTPKPLVDFCNKPILLHQVEALAAAGVDHVILAVSYMSQVLEKEMKAQEQRLGIRISMSHEEEPLGTAGPLALARDLLSETADPFFVLNSDVICDFPFQAMVQFHRHHGQEGSILVTKVEEPSKYGVVVCEADTGRIHRFVEKPQVFVSNKINAGMYILSPAVLRRIQLQPTSIEKEVFPIMAKEGQLYAMELQGFWMDIGQPKDFLTGMCLFLQSLRQKQPERLCSGPGIVGNVLVDPSARIGQNCSIGPNVSLGPGVVVEDGVCIRRCTVLRDARIRSHSWLESCIVGWRCRVGQWVRMENVTVLGEDVIVNDELYLNGASVLPHKSIGESVPEPRIIM

• Full Name: GDP-mannose pyrophosphorylase B
• Calculated Molecular Weight: 40 kDa
• Observed Molecular Weight: 40-43 kDa
• GenBank Accession Number: BC001141
• Gene Symbol: GMPPB
• Gene ID (NCBI): 29925
• RRID: AB_2111064
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y5P6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GMPPB is a cytoplasmic enzyme that catalyzes the formation of GDP-mannose, a key substrate for several glycosylation pathways, including O-mannosylation of α-DG. GMPPB facilitates the formation of the sugar donor GDP-mannose and pyrophosphate from mannose-1-phosphate and GTP, a very early step in the glycosylation pathway (PMID: 37834154, 27147698). GMPPB mutations correlate with several congenital diseases, including severe congenital muscular dystrophy (CMD) with abnormalities in the brain and eye (PMID: 26310427, 30684953). Western blot analysis detected GMPPB at an apparent molecular mass of 40 kDa.

Publications

What published studies show

The GMPPB Polyclonal antibody (Cat# 15094-1-AP) has 9 citations published in journals including Nature Communications, Advanced Science, Molecular Biomedicine, International Journal of Molecular Sciences, Frontiers in Nutrition, iScience, Frontiers in Molecular Neuroscience, and Frontiers in Molecular Biosciences. Research fields span glycosylation disorders, dystroglycanopathy, neuromuscular development, esophageal cancer, glioblastoma, depression, aging, and GDP-mannose metabolism.

Automatically generated from the 9 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB,IF	Nat Commun; Gmppb-mutant mice exhibit dystroglycanopathy symptoms that are rescued with GSK3β inhibition or AAV-mediated GMPPB gene replacement.; Authors - Ziwei Fu
human	IHC	Adv Sci (Weinh); Spatio-Temporal Proteomic Landscape Reveals Early Warning Signals of Esophageal Squamous Cell Carcinoma Progression.; Authors - Xumiao Li
zebrafish	WB	Mol Biomed; GMPPB-congenital disorders of glycosylation associate with decreased enzymatic activity of GMPPB.; Authors - Zhe Liu
human	WB,IHC	Int J Mol Sci; Silencing GMPPB Inhibits the Proliferation and Invasion of GBM via Hippo/MMP3 Pathways; Authors - Zi-Lu Huang ; KD Validated
human,mouse	WB	Front Nutr; Altered mannose metabolism in chronic stress and depression is rapidly reversed by vitamin B12; Authors - Patricia Franzka
human	WB	iScience; A Trace Amount of Galactose, a Major Component of Milk Sugar, Allows Maturation of Glycoproteins during Sugar Starvation.; Authors - Norio Sasaoka