GNAQ Polyclonal antibody

GNAQ Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 27264-1-AP

Synonyms

G ALPHA q, GAQ, GNAQ

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Validation Data Gallery

• 

  WB analysis of PC-12 using 27264-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 27264-1-AP (GNAQ antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 27264-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 27264-1-AP (GNAQ Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 27264-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 27264-1-AP (GNAQ antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: PC-12 cells, HepG2 cells, mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 27264-1-AP is GNAQ Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GNAQ fusion protein Ag26170
• Full Name: guanine nucleotide binding protein (G protein), q polypeptide
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 38-40 kDa
• GenBank Accession Number: BC057777
• Gene Symbol: GNAQ
• Gene ID (NCBI): 2776
• RRID: AB_2880822
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GNAQ is a proto-oncogene closely related to the α subunit of guanine nucleotide-binding proteins (G proteins) that serve as key intermediates between membrane-bound G-protein-coupled receptors (GPCRs) and GPCR signalling nodes. Aberrant expression and activity of G proteins and GPCRs are frequently associated with tumorigenesis. Recent studies show that oncogenic activating mutations in GNAQ are present in 80% of the melanomas. The calculated molecular weight of GNAQ is 42 kDa. This antibody can detect a band of 38-40 kDa in SDS-PAGE. (PMID: 33993733)