GNGT1 Polyclonal antibody

GNGT1 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 11884-1-AP

Synonyms

GNG1, GNGT1, Transducin gamma chain

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Validation Data Gallery

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  WB analysis of mouse eye using 11884-1-AP

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 11884-1-AP (GNGT1 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 11884-1-AP

  Immunofluorescent analysis of HeLa cells using 11884-1-AP (GNGT1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse eye tissue
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11884-1-AP targets GNGT1 in WB, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GNGT1 fusion protein Ag2488
• Full Name: guanine nucleotide binding protein (G protein), gamma transducing activity polypeptide 1
• Calculated Molecular Weight: 74 aa, 8 kDa
• Observed Molecular Weight: 8 kDa
• GenBank Accession Number: BC029367
• Gene Symbol: GNGT1
• Gene ID (NCBI): 2792
• RRID: AB_10858628
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

G protein subunit gamma-T1(GNGT1) is encoded by the transducin gamma-subunit gene, which localized on huaman chromosome 7q21.3, participating in various transmembrane signaling systems. Particularly, GNGT1 gene is specific to retinal rod photoreceptors. Present polyclonal anti-GNGT1 antibody (11884-1-AP) is produced by immunizing rabbits with full-length chain of GNGT1 and dectects an 8 kDa band in mouse retinal tissue.