GNPNAT1 Polyclonal antibody

• Featured Product
• KD/KO Validated

GNPNAT1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 16282-1-AP

Synonyms

GNA1, GNPNAT, GNPNAT1, Gpnat1, Phosphoglucosamine acetylase

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Validation Data Gallery

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  WB analysis using 16282-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16282-1-AP (GNPNAT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse large intestine using 16282-1-AP

  mouse large intestine tissue were subjected to SDS PAGE followed by western blot with 16282-1-AP (GNPNAT1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse large intestine using 16282-1-AP

  IP result of anti-GNPNAT1 (IP:16282-1-AP, 3ug; Detection:16282-1-AP 1:300) with mouse large intestine tissue lysate 3200ug.

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  IHC staining of human liver using 16282-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16282-1-AP (GNPNAT1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 16282-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16282-1-AP (GNPNAT1 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: MCF-7 cells, mouse large intestine tissue, MDA-MB-231 cells, mouse colon tissue
• Positive IP detected in: mouse large intestine tissue
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 2 publications below

Product Information

16282-1-AP targets GNPNAT1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GNPNAT1 fusion protein Ag9375
• Full Name: glucosamine-phosphate N-acetyltransferase 1
• Calculated Molecular Weight: 184 aa, 21 kDa
• Observed Molecular Weight: 21-23 kDa
• GenBank Accession Number: BC012179
• Gene Symbol: GNPNAT1
• Gene ID (NCBI): 64841
• RRID: AB_2110243
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Glucosamine-6 phosphate-N-acetyl Transferase (GNPNAT1) is a key enzyme in the hexosamine biosynthetic pathway. It converts D-glucosamine 6-phosphate to N-acetyl-D-glucosamine 6-phosphate, the acetylation step allowing carbon and nitrogen enter into the hexosamine biosynthetic pathway. Recently inhibition of GNPNAT1 has been reported to promote proliferation and aggressiveness of castration-resistant prostate cancer. (27194471) GNPNAT1 is a small dimeric protein localized in the Golgi and endosome membranes.

Publications

Species	Application	Title
human	WB	Cell Res; Mannose antagonizes GSDME-mediated pyroptosis through AMPK activated by metabolite GlcNAc-6P; Authors - Yuan-Li Ai
human	WB,IHC	Nat Commun; Inhibition of the hexosamine biosynthetic pathway promotes castration-resistant prostate cancer.; Authors - Akash K Kaushik ; KD Validated
human	IHC	Front Mol Biosci; Upregulation of GNPNAT1 Predicts Poor Prognosis and Correlates With Immune Infiltration in Lung Adenocarcinoma.; Authors - Wenting Liu
human	WB	Front Immunol; GNPNAT1 is a potential biomarker correlated with immune infiltration and immunotherapy outcome in breast cancer; Authors - Renjie Yuan
mouse	WB	Int J Mol Sci; ER Stress-Perturbed Intracellular Protein O-GlcNAcylation Aggravates Podocyte Injury in Diabetes Nephropathy; Authors - Shicong Song