Tested Applications
| Positive WB detected in | HepG2 cells |
| Positive IP detected in | BxPC-3 cells |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16700-1-AP targets Glypican 1 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10095 Product name: Recombinant human GPC1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 208-558 aa of BC051279 Sequence: ELRLRATRAFVAARSFVQGLGVASDVVRKVAQVPLGPECSRAVMKLVYCAHCLGVPGARPCPDYCRNVLKGCLANQADLDAEWRNLLDSMVLITDKFWGTSGVESVIGSVHTWLAEAINALQDNRDTLTAKVIQGCGNPKVNPQGPGPEEKRRRGKLAPRERPPSGTLEKLVSEAKAQLRDVQDFWISLPGTLCSEKMALSTASDDRCWNGMARGRYLPEVMGDGLANQINNPEVEVDITKPDMTIRQQIMQLKIMTNRLRSAYNGNDVDFQDASDDGSGSGSGDGCLDDLCGRKVSRKSSSSRTPLTHALPGLSEQEGQKTSAASCPQPPTFLLPLLLFLALTVARPRWR Predict reactive species |
| Full Name | glypican 1 |
| Calculated Molecular Weight | 558 aa, 62 kDa |
| Observed Molecular Weight | 65 kDa |
| GenBank Accession Number | BC051279 |
| Gene Symbol | GPC1 |
| Gene ID (NCBI) | 2817 |
| RRID | AB_1851168 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P35052 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Glypicans are a family of HSPGs that are attached to the cell membrane by a glycosyl-phosphatidylinositol anchor. Glypicans are considered to have the ability to modulate the activities of HBGFs. The expression of glypicans is regulated temporally and spatially during development, suggesting they are involved in development and morphogenesis. To date, six glypicans (GPC1 to GPC6) have been identified in mammals. GPC1 is most ubiquitously expressed in adult tissues. GPC1 promotes efficient signaling by HBGFs and plays a critical role in cell growth and differentiation. GPC1 is involved in tumorigenesis and angiogenesis, and is frequently overexpressed in several types of tumors including pancreatic carcinoma, breast cancer and glioma.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Glypican 1 antibody 16700-1-AP | Download protocol |
| IP protocol for Glypican 1 antibody 16700-1-AP | Download protocol |
| WB protocol for Glypican 1 antibody 16700-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-GPC1 (Glypican-1) antibody (Cat# 16700-1-AP) has 26 citations across journals including Nature Biomedical Engineering, Nature Communications, Advanced Science, Cell Death & Disease, Modern Pathology, Matrix Biology, Scientific Reports, and PLoS One. Research fields span cancer biology (glioma, colorectal, hepatocellular, lung, and yolk sac tumors), osteoporosis, diabetic nephropathy, hair follicle biology, aging, endothelial/vascular biology, extracellular vesicle detection, and traumatic shock.
| Species | Application | Title |
|---|---|---|
Nat Biomed Eng Rapid and sensitive detection of cancer-derived small extracellular vesicles using Janus particles. | ||
Nat Biomed Eng Sulfated polysaccharides target RANK Lys97 to inhibit osteoclast differentiation and reverse osteoporosis | ||
Nat Commun Decoding non-canonical mRNA decay by the endoplasmic-reticulum stress sensor IRE1α | ||
Adv Sci (Weinh) Isoquercitrin Alleviates Diabetic Nephropathy by Inhibiting STAT3 Phosphorylation and Dimerization | ||
Cell Death Dis Overexpression of Annexin A2 promotes proliferation by forming a Glypican 1/c-Myc positive feedback loop: prognostic significance in human glioma.
| ||
Mater Today Bio Biomimetic flowing vessel-on-a-chip recruiting glycocalyx features for investigating dexmedetomidine function. |
Reviews
What customers say
The product received a 5-star rating for its use in immunohistochemistry on lung mesothelioma tissue at a 1:100 dilution. The reviewer reported that the antibody performed well enough to support a publication that was subsequently accepted in Modern Pathology, indicating reliable staining quality and reproducibility for their research needs.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Vishwa Jeet (Verified Customer) (11-09-2017) | Recently, our publication has been accepted in Modern Pathology.
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