Validation Data Gallery
|Positive WB detected in||HeLa cells, PC-3 cells, PC3 cells, U251 cells, U87-MG cells|
|Positive IHC detected in||human lung cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||PC-3 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:100-1:400|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
15171-1-AP targets GPI in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||GPI fusion protein Ag7423|
|Full Name||glucose phosphate isomerase|
|Calculated molecular weight||63 kDa|
|Observed molecular weight||55-64 kDa|
|GenBank accession number||BC004982|
|Gene ID (NCBI)||2821|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
GPI(Glucose-6-phosphate isomerase), which is also named as autocrine motility factor (AMF), phosphoglucose isomerase(PGI), Neuroleukinis(NLK), phosphohexose isomerase(PHI) or sperm antigen 36(SA-36), is a housekeeping cytosolic enzyme that plays a key role in both glycolysis and gluconeogenesis pathways. It is also a multifunctional protein that displays cytokine properties, eliciting mitogenic, motogenic, and differentiation activities, and has been implicated in tumor progression and metastasis (PMID:12783864, 19603112). This protein can exsit as a homodimer in the catalytically active form and a monomer in the secreted form (PMID:11371164). It has 2 isoforms produced by alternative splicing with the calculated molecular mass of 63-64kDa, and an apparent molecular mass of 55 and 64 kDa under non-reducing and reducing conditions, respectively(PMID: 19603112, 11004567).
J Cell Mol Med
Low levels of AMPK promote epithelial-mesenchymal transition in lung cancer primarily through HDAC4- and HDAC5-mediated metabolic reprogramming.
Cell Death Dis
Reciprocal regulation of LOXL2 and HIF1α drives the Warburg effect to support pancreatic cancer aggressiveness.
Stem Cells Int
Schwann Cells Accelerate Osteogenesis via the Mif/CD74/FOXO1 Signaling Pathway In Vitro.
Recent Pat Anticancer Drug Discov
Alpha-solanine anti-tumor effects in non-small cell lung cancer through regulating the energy metabolism pathway.
Valproic acid Suppresses Breast Cancer Cell Growth Through Triggering Pyruvate Kinase M2 Isoform Mediated Warburg Effect.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Kristian (Verified Customer) (07-26-2021)
GPI is shown in green. Nuclei are shown in blue.
Daniel (Verified Customer) (02-06-2020)
Denatured lysate was separated on a 4-20% PAGE