Tested Applications
| Positive WB detected in | HeLa cells, PC-3 cells, PC3 cells, U251 cells, U87-MG cells |
| Positive IHC detected in | human lung cancer tissue, human normal colon Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | PC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15171-1-AP targets GPI in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7423 Product name: Recombinant human GPI protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-272 aa of BC004982 Sequence: MAALTRDPQFQKLQQWYREHRSELNLRRLFDANKDRFNHFSLTLNTNHGHILVDYSKNLVTEDVMRMLVDLAKSRGVEAARERMFNGEKINYTEGRAVLHVALRNRSNTPILVDGKDVMPEVNKVLDKMKSFCQRVRSGDWKGYTGKTITDVINIGIGGSDLGPLMVTEALKPYSSGGPRVWYVSNIDGTHIAKTLAQLNPESSLFIIASKTFTTQETITNAETAKEWFLQAAKDPSAVAKHFVALSTNTTKVKEFGIDPQNMFEFWDWVGG Predict reactive species |
| Full Name | glucose phosphate isomerase |
| Calculated Molecular Weight | 63 kDa |
| Observed Molecular Weight | 55-64 kDa |
| GenBank Accession Number | BC004982 |
| Gene Symbol | GPI |
| Gene ID (NCBI) | 2821 |
| RRID | AB_2263537 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P06744 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GPI(Glucose-6-phosphate isomerase), which is also named as autocrine motility factor (AMF), phosphoglucose isomerase(PGI), Neuroleukinis(NLK), phosphohexose isomerase(PHI) or sperm antigen 36(SA-36), is a housekeeping cytosolic enzyme that plays a key role in both glycolysis and gluconeogenesis pathways. It is also a multifunctional protein that displays cytokine properties, eliciting mitogenic, motogenic, and differentiation activities, and has been implicated in tumor progression and metastasis (PMID:12783864, 19603112). This protein can exsit as a homodimer in the catalytically active form and a monomer in the secreted form (PMID:11371164). It has 2 isoforms produced by alternative splicing with the calculated molecular mass of 63-64kDa, and an apparent molecular mass of 55 and 64 kDa under non-reducing and reducing conditions, respectively(PMID: 19603112, 11004567).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for GPI antibody 15171-1-AP | Download protocol |
| IHC protocol for GPI antibody 15171-1-AP | Download protocol |
| WB protocol for GPI antibody 15171-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GPI (Glucose-6-phosphate isomerase) polyclonal antibody, catalog number 15171-1-AP, has 42 citations. These appear in journals including Cell Metabolism, Cell Research, Nature Metabolism, Nature Neuroscience, EMBO Journal, EMBO Reports, Cell Reports, Molecular Psychiatry, Science Reports, and others. Research fields span cancer metabolism (glycolysis/Warburg effect), Alzheimer's disease, neuroinflammation, ferroptosis/cuproptosis, immune regulation, and proteomics, with applications mainly in Western blot, plus IHC and IF.
| Species | Application | Title |
|---|---|---|
Cell Metab The diapause-like colorectal cancer cells induced by SMC4 attenuation are characterized by low proliferation and chemotherapy insensitivity | ||
Cell Res Mannose antagonizes GSDME-mediated pyroptosis through AMPK activated by metabolite GlcNAc-6P | ||
Cell Res cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions. | ||
Nat Metab Targeting aldolase A in hepatocellular carcinoma leads to imbalanced glycolysis and energy stress due to uncontrolled FBP accumulation | ||
J Adv Res Sodium citrate targeting Ca2+/CAMKK2 pathway exhibits anti-tumor activity through inducing apoptosis and ferroptosis in ovarian cancer |
Reviews
What customers say
All three reviews rate the antibody 5 out of 5 stars. Users report successful performance in Western Blot (at 1:1000 and 1:2000 dilutions) and Immunofluorescence (at 1:200). Tested samples include NRVCMs, RCC4 cells, and mouse kidney tissue. One reviewer described it as "a great antibody for NRVCMs," while others confirmed clear GPI signal detection in both denatured lysates and fixed cells. Overall, the antibody is well-received for reliable target recognition across multiple applications and sample types.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Marco (Verified Customer) (07-04-2024) | A great antibody for NRVCMs
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Kristian (Verified Customer) (07-26-2021) | GPI is shown in green. Nuclei are shown in blue.
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Daniel (Verified Customer) (02-06-2020) | Denatured lysate was separated on a 4-20% PAGE
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