GPR107 Polyclonal antibody

• Featured Product
• KD/KO Validated

GPR107 Polyclonal Antibody for WB, IF, FC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, FC, IHC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 25076-1-AP

Synonyms

G protein coupled receptor 107, GCDRP, GPR107, KIAA1624, LUSTR1, Protein GPR107, RP11 88G17

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Validation Data Gallery

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  WB analysis of HEK-293 using 25076-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 25076-1-AP (GPR107 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse cerebellum using 25076-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 25076-1-AP (GPR107 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of HEK-293 using 25076-1-AP

  Immunofluorescent analysis of HEK-293 cells using 25076-1-AP (GPR107 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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  FC experiment of SH-SY5Y using 25076-1-AP

  1X10^6 SH-SY5Y cells were stained with 0.2ug GPR107 antibody (25076-1-AP, red) and control antibody (blue). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse cerebellum tissue
• Positive IF detected in: HEK-293 cells
• Positive FC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF): IF : 1:10-1:100
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

The immunogen of 25076-1-AP is GPR107 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GPR107 fusion protein Ag18649
• Full Name: G protein-coupled receptor 107
• Calculated Molecular Weight: 552 aa, 67 kDa
• Observed Molecular Weight: 60-67 kDa
• GenBank Accession Number: BC110518
• Gene Symbol: GPR107
• Gene ID (NCBI): 57720
• RRID: AB_2879886
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GPR107 also named as KIAA1624 or LUSTR1 is 600 amino acid protein, which belongs to the LU7TM family. GPR107 localizes in membrane and is a multi-pass membrane protein. GPR107 is a promising candidate receptor for neuronostatin, and neuronostatin, interacting with GPR107, may play an important role in the central control of cardiovascular function.

Publications

Species	Application	Title
mouse	WB	Nat Chem Biol; A proteome-wide map of 20(S)-hydroxycholesterol interactors in cell membranes.; Authors - Yu-Shiuan Cheng
human	WB,IHC,IF	J Exp Clin Cancer Res; LncGPR107 drives the self-renewal of liver tumor initiating cells and liver tumorigenesis through GPR107-dependent manner.; Authors - Guanqun Huang
mouse	WB	Neurochem Res; Neuronostatin Promotion Soluble Aβ1-42 Oligomers: Induced Dysfunctional Brain Glucose Metabolism in Mice.; Authors - Shaobin Yang
mouse	WB	Behav Brain Res; Neuronostatin promotes soluble Aβ1-42 oligomers -induced spatial learning and memory impairments in mice.; Authors - Shaobin Yang ; KD Validated
human	WB	Nat Chem Biol; Chemoproteomics reveals microbiota-derived aromatic monoamine agonists for GPRC5A; Authors - Xiaohui Zhao