Tested Applications
| Positive WB detected in | HEK-293 cells, mouse cerebellum tissue |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:300-1:1200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25076-1-AP targets GPR107 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag18649 Product name: Recombinant human GPR107 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 41-260 aa of BC110518 Sequence: VHHLALKDDVRHKVHLNTFGFFKDGYMVVNVSSLSLNEPEDKDVTIGFSLDRTKNDGFSSYLDEDVNYCILKKQSVSVTLLILDISRSEVRVKSPPEAGTQLPKIIFSRDEKVLGQSQEPNVNPASAGNQTQKTQDGGKSKRSTVDSKAMGEKSFSVHNNGGAVSFQFFFNISTDDQEGLYSLYFHKCLGKELPSDKFTFSLDIEITEKNPDSYLSAGEI Predict reactive species |
| Full Name | G protein-coupled receptor 107 |
| Calculated Molecular Weight | 552 aa, 67 kDa |
| Observed Molecular Weight | 60-67 kDa |
| GenBank Accession Number | BC110518 |
| Gene Symbol | GPR107 |
| Gene ID (NCBI) | 57720 |
| RRID | AB_2879886 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5VW38 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GPR107 also named as KIAA1624 or LUSTR1 is 600 amino acid protein, which belongs to the LU7TM family. GPR107 localizes in membrane and is a multi-pass membrane protein. GPR107 is a promising candidate receptor for neuronostatin, and neuronostatin, interacting with GPR107, may play an important role in the central control of cardiovascular function.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for GPR107 antibody 25076-1-AP | Download protocol |
| WB protocol for GPR107 antibody 25076-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GPR107 antibody (Cat# 25076-1-AP) has 10 total citations spanning journals including Blood, Nature Chemical Biology, Journal of Experimental & Clinical Cancer Research, Cancer Gene Therapy, Neuropharmacology, Neurochemical Research, Behavioral and Brain Research, and bioRxiv. Research fields cover oncology (AML, liver tumorigenesis, breast cancer metastasis), neuroscience (Alzheimer's disease, glucose homeostasis, neuronal metabolism), chemoproteomics, and membrane proteomics. Applications include WB, IHC, IF, and CoIP in human and mouse models.
| Species | Application | Title |
|---|---|---|
Blood Targeting PRMT9 Overcomes Venetoclax Resistance in AML by Modulating Splicing and Inhibiting Translation | ||
Nat Chem Biol A proteome-wide map of 20(S)-hydroxycholesterol interactors in cell membranes. | ||
Nat Chem Biol Chemoproteomics reveals microbiota-derived aromatic monoamine agonists for GPRC5A | ||
J Exp Clin Cancer Res LncGPR107 drives the self-renewal of liver tumor initiating cells and liver tumorigenesis through GPR107-dependent manner. | ||
Cancer Gene Ther GPR107: A key driver of breast cancer invasion and metastasis through collagen IV modulation.
| ||
Neuropharmacology Cyclic neuronostatin regulates glucose homeostasis and food intake through GPR107 phosphorylation. |
