Tested Applications
| Positive WB detected in | HEK-293 cells, mouse brain tissue, rat brain tissue, HeLa cells, U-251 cells, U-87 MG cells |
| Positive IP detected in | mouse liver tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17950-1-AP targets GPR177 in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, goat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12428 Product name: Recombinant human GPR177 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 34-239 aa of BC110826 Sequence: LIAPGPTTAVSYMSVKCVDARKNHHKTKWFVPWGPNHCDKIRDIEEAIPREIEANDIVFSVHIPLPHMEMSPWFQFMLFILQLDIAFKLNNQIRENAEVSMDVSLAYRDDAFAEWTEMAHERVPRKLKCTFTSPKTPEHEGRYYECDVLPFMEIGSVAHKFYLLNIRLPVNEKKKINVGIGEIKDIRLVGIHQNGGFTKVWFAMKT Predict reactive species |
| Full Name | G protein-coupled receptor 177 |
| Calculated Molecular Weight | 541 aa, 62 kDa |
| Observed Molecular Weight | 45-60 kDa |
| GenBank Accession Number | BC110826 |
| Gene Symbol | GPR177 |
| Gene ID (NCBI) | 79971 |
| RRID | AB_2034928 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5T9L3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GPR177, also known as Wntless (WLS) or Evi, is an evolutionarily conserved transmembrane protein necessary for Wnt protein secretion. GPR177 localizes to compartments of the secretory pathway, including the ER, Golgi apparatus, endosomes, and plasma membrane. GPR177 is overexpressed in several cancer types including glioma and gastric cancer. Several isoforms of GPR177 exist due to the alternative splicing, with predicted MW around 62 and 51 kDa, respectively. The sequence can be processed by removing the signal peptide, which generates a mature form of 45-46 kDa. In addition, slow migragrating band above 62 kDa can also be detected because GPR177 is modified by N-linked glycosylation (PMID: 20549736).
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for GPR177 antibody 17950-1-AP | Download protocol |
| WB protocol for GPR177 antibody 17950-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GPR177 Polyclonal antibody (Cat# 17950-1-AP) has 12 citations spanning journals including Cell Research, Kidney International, Autophagy, Molecular Cell, ACS Chemical Biology, iScience, Cells, Frontiers in Physiology, Reproductive Sciences, Onco Targets and Therapy, Journal of Ethnopharmacology, and International Ophthalmology. Research fields cover Wnt/β-catenin signaling, cell fate specification, renal fibrosis, autophagy, endosomal trafficking, ER protein quality control, hair follicle morphogenesis, skeletal muscle repair, breast cancer prognosis, and scleral remodeling.
| Species | Application | Title |
|---|---|---|
Cell Res Comprehensive profiling reveals mechanisms of SOX2-mediated cell fate specification in human ESCs and NPCs. | ||
Kidney Int Non-canonical Wnt/calcium signaling is protective against podocyte injury and glomerulosclerosis | ||
Autophagy WNT2B impairs endosomal trafficking via WASHC5 to inhibit autophagy: a novel non-secretory WNT pathway. | ||
Mol Cell TMUB1 is an endoplasmic reticulum-resident escortase that promotes the p97-mediated extraction of membrane proteins for degradation | ||
J Ethnopharmacol Shugan Tongbian Decoction ameliorates constipation and mental disorder by inhibiting UPR(mt) via Wnt/Retromer axis. | ||
Cells Integrative Analysis of Methylome and Transcriptome Reveals the Regulatory Mechanisms of Hair Follicle Morphogenesis in Cashmere Goat. |
Reviews
What customers say
The product received a 5-star rating from a researcher who used it for Western Blot at a 1:1000 dilution on liver tissue. The reviewer described the antibody as "good for WB," indicating satisfactory performance in their application. Overall, the feedback reflects a positive experience with the antibody's reliability for Western Blot experiments.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
MALLIKARJUNA (Verified Customer) (11-13-2025) | good for WB
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