GPR50 Polyclonal antibody

• Featured Product
• KD/KO Validated

GPR50 Polyclonal Antibody for IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, FC, IHC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 19762-1-AP

Synonyms

G protein coupled receptor 50, GPR50, H9, Mel1c, Melatonin related receptor

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Validation Data Gallery

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  IHC staining of mouse brain using 19762-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19762-1-AP (GPR50 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of mouse brain using 19762-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19762-1-AP (GPR50 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of mouse brain tissue using 19762-1-AP

  IF result of anti-GPR50 (19762-1-AP, 1:50) with mouse brain tissue slide by Dr. Qian Li, University of Edinburgh.

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  IF Staining of SH-SY5Y cells using 19762-1-AP

  IF result of anti-GPR50 (19762-1-AP, 1:500) with SH-SY5Y cells transfected with GPR50 by Dr. Qian Li, University of Edinburgh.

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  IF Staining of mouse brain using 19762-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using GPR50 antibody (19762-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse brain using 19762-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using GPR50 antibody (19762-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of K-562 using 19762-1-AP

  1X10^6 K-562 cells were stained with 0.2ug GPR50 antibody (19762-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Tested Applications

• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: mouse brain tissue, SH-SY5Y cells
• Positive FC detected in: K-562 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 4 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

19762-1-AP targets GPR50 in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: G protein-coupled receptor 50
• Calculated Molecular Weight: 67 kDa
• GenBank Accession Number: NM_004224
• Gene Symbol: GPR50
• Gene ID (NCBI): 9248
• RRID: AB_2878603
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GPR50, also known as Melatonin-related receptor or H9, is a member of the G-protein coupled receptor 1 family. It is closely related to the G protein-coupled melatonin receptor family and has been demonstrated as the mammalian ortholog of Mel1c receptor (PMID: 8647286; 18400093). GPR50 does not bind melatonin. It is able to heterodimerize with both the MT1 (Mel1a) and MT2 (Mel1b) melatonin receptor subtypes. While GPR50 has no effect on MT2 function, GPR50 prevented MT1 from both binding melatonin and coupling to G proteins. GPR50 is involved in neuroendocrine function and plays a role in the regulation of energy metabolism (PMID: 8647286; 17957037).

Publications

Species	Application	Title
human	WB	Nat Cell Biol; Molecular identification of a BAR domain-containing coat complex for endosomal recycling of transmembrane proteins.; Authors - Boris Simonetti
mouse	WB,IP	Transl Stroke Res; Melatonin Offers Dual-Phase Protection to Brain Vessel Endothelial Cells in Prolonged Cerebral Ischemia-Recanalization Through Ameliorating ER Stress and Resolving Refractory Stress Granule; Authors - Danli Lu
human	IF	Front Pharmacol; An Improved Genetically Encoded Fluorescent cAMP Indicator for Sensitive cAMP Imaging and Fast Drug Screening.; Authors - Wenfeng Liu ; KO Validated
human	WB	Am J Chin Med; Tetramethylpyrazine Attenuated Sevoflurane-Induced Neurotoxicity by Enhancing Autophagy through GPR50/CREB Pathway in SH-SY5Y Cells.; Authors - Lili Xu ; KD Validated
mouse	WB	FEBS Open Bio; Orphan receptor GPR50 attenuates inflammation and insulin signaling in 3T3-L1 preadipocytes; Authors - Zhenyu Yao ; KO Validated

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