Tested Applications
Positive FC (Intra) detected in | RAW 264.7 cells |
Recommended dilution
Application | Dilution |
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Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.8 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
FITC-20306 targets GPR65 in ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | GPR65 fusion protein Ag14139 Predict reactive species |
Full Name | G protein-coupled receptor 65 |
Calculated Molecular Weight | 337 aa, 39 kDa |
GenBank Accession Number | BC035633 |
Gene Symbol | GPR65 |
Gene ID (NCBI) | 8477 |
Conjugate | FITC Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 498 nm / 526 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q8IYL9 |
Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GPR65 (G protein-coupled receptor 65) was defined originally as T-cell death-associated gene 8 (TDAG8), functions as a proton-sensing receptor, and is also sensitive to psychosine (PMID: 35343079). GPR65 promotes adaptation to an acidic environment to enhance cell survival and proliferation, thereby promoting tumor development (PMID: 36852075).
Protocols
Product Specific Protocols | |
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FC protocol for FITC GPR65 antibody FITC-20306 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |