Tested Applications
| Positive WB detected in | A431 cells, Jurkat cells, THP-1 cells, AC16 cells |
| Positive IP detected in | THP-1 cells |
| Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, A431 cells, PC-3 cells |
| Positive FC (Intra) detected in | Jurkat cells |
This antibody can only dectect full length GSDMD.
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20770-1-AP targets GSDMD in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, rabbit, chicken |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag14757 Product name: Recombinant human GSDMD protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 135-484 aa of BC008904 Sequence: HERHLRQPEHKVLQQLRSRGDNVYVVTEVLQTQKEVEVTRTHKREGSGRFSLPGATCLQGEGQGHLSQKKTVTIPSGSTLAFRVAQLVIDSDLDVLLFPDKKQRTFQPPATGHKRSTSEGAWPQLPSGLSMMRCLHNFLTDGVPAEGAFTEDFQGLRAEVETISKELELLDRELCQLLLEGLEGVLRDQLALRALEEALEQGQSLGPVEPLDGPAGAVLECLVLSSGMLVPELAIPVVYLLGALTMLSETQHKLLAEALESQTLLGPLELVGSLLEQSAPWQERSTMSLPPGLLGNSWGEGAPAWVLLDECGLELGEDTPHVCWEPQAQGRMCALYASLALLSGLSQEPH Predict reactive species |
| Full Name | gasdermin D |
| Calculated Molecular Weight | 484 aa, 53 kDa |
| Observed Molecular Weight | 50-53 kDa |
| GenBank Accession Number | BC008904 |
| Gene Symbol | GSDMD |
| Gene ID (NCBI) | 79792 |
| RRID | AB_10696319 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P57764 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GSDMD is a 53 kDa cytosolic protein and a member of the gasdermin protein family, which features 6 members in humans (GSDMA, -B, -C, -D, -E and -F. GSDMD was identified as the executioner of inflammasome-associated pyroptosis. Caspase-1, -11, or -4 cleave GSDMD after an aspartate residue within a central linker domain (D275 in humans and D276 in mice) to generate a 31-kDa N-terminal GSDMD fragment (GSDMD-NT) and a 22-kDa C-terminal fragment (GSDMD-CT). In addition to the canonical 31/22 kDa cleavage products, a 43 kDa fragment has been observed under specific experimental or pathological conditions. The generation of the 43 kDa fragment was observed upon caspase-3 and -7 activation during apoptosis. This antibody can only detect full-length GSDMD.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for GSDMD antibody 20770-1-AP | Download protocol |
| IF protocol for GSDMD antibody 20770-1-AP | Download protocol |
| IHC protocol for GSDMD antibody 20770-1-AP | Download protocol |
| IP protocol for GSDMD antibody 20770-1-AP | Download protocol |
| WB protocol for GSDMD antibody 20770-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-Gasdermin D (GSDMD) antibody (Cat# 20770-1-AP) has 602 total citations. Citations appear in high-impact journals including Nature, Cell, Nature Communications, Nature Cell Biology, Nature Cancer, Science Advances, Molecular Cell, Journal of Experimental Medicine, Cell Reports, Autophagy, and many others. Research fields span pyroptosis, NLRP3 inflammasome activation, ferroptosis, PANoptosis, neuroinflammation, oncology, cardiovascular disease, liver/kidney injury, sepsis, spinal cord injury, Alzheimer's and Parkinson's disease, atherosclerosis, nanomedicine/drug delivery, and traditional Chinese medicine.
| Species | Application | Title |
|---|---|---|
Nature Inflammasome-activated gasdermin D causes pyroptosis by forming membrane pores.
| ||
Cell Type III interferons induce pyroptosis in gut epithelial cells and impair mucosal repair | ||
Cell Res Gasdermin D is an executor of pyroptosis and required for interleukin-1β secretion. | ||
Adv Mater Efficient Delivery of Lomitapide Using Hybrid Membrane-Coated Tetrahedral DNA Nanostructures for Glioblastoma Therapy | ||
Cell Mol Immunol NLRP3-dependent pyroptosis is required for HIV-1 gp120-induced neuropathology. |
Reviews
What customers say
All three reviewers gave 5-star ratings. The antibody performed excellently for Western blot and IHC, producing very clear bands at dilutions ranging from 1:300 to 1:1,000. One reviewer also confirmed successful use in immunofluorescence and immunoprecipitation. Overall, users were highly satisfied with the results, with one noting that the antibody's performance even contributed to securing a research grant.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Mounika (Verified Customer) (01-08-2026) | Antibodies worked excellently for western blots and IHC, we also got the grant for this research project ! Amazing!
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Mounika (Verified Customer) (01-08-2026) | Mostly used for Western blots, bands are very excellent
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Isha (Verified Customer) (03-02-2021) | Bands were so clear. Could not refrain myself from writing this review.
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