GSDMD Polyclonal antibody

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  WB analysis of Jurkat using 20770-1-AP

  WB result of GSDMD antibody (20770-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GSDMD transfected Jurkat cells.

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  WB analysis using 20770-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20770-1-AP (GSDMD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 20770-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20770-1-AP (GSDMD antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of THP-1 using 20770-1-AP

  IP result of anti-GSDMD (IP:20770-1-AP, 4ug; Detection:20770-1-AP 1:4000) with THP-1 cells lysate 1560 ug.

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  IHC staining of human ovary cancer using 20770-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 20770-1-AP (GSDMD antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 20770-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 20770-1-AP (GSDMD antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 20770-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 20770-1-AP (GSDMD antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 20770-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 20770-1-AP (GSDMD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 20770-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GSDMD antibody (20770-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HeLa using 20770-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GSDMD antibody (20770-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of Jurkat using 20770-1-AP

  1x10^6 Jurkat cells were intracellularly stained with 0.4 ug GSDMD Polyclonal antibody (20770-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

• Featured Product
• KD/KO Validated

GSDMD Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 20770-1-AP

Publications(380)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (5)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

FKSG10, gasdermin D, Gasdermin domain-containing protein 1, gasderminD, Gasdermin-D

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Tested Applications

• Positive WB detected in: A431 cells, NIH/3T3 cells, Jurkat cells, C6 cells, rat liver tissue, rat brain tissue
• Positive IP detected in: THP-1 cells
• Positive IHC detected in: human ovary cancer tissue, mouse lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: Jurkat cells

This antibody can only dectect full length GSDMD.

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 8 publications below
• WB: See 298 publications below
• IHC: See 74 publications below
• IF: See 103 publications below
• IP: See 1 publications below

Product Information

20770-1-AP targets GSDMD in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, rabbit, zebrafish, bovine, hamster
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag14757

  Product name: Recombinant human GSDMD protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 135-484 aa of BC008904

  Sequence: HERHLRQPEHKVLQQLRSRGDNVYVVTEVLQTQKEVEVTRTHKREGSGRFSLPGATCLQGEGQGHLSQKKTVTIPSGSTLAFRVAQLVIDSDLDVLLFPDKKQRTFQPPATGHKRSTSEGAWPQLPSGLSMMRCLHNFLTDGVPAEGAFTEDFQGLRAEVETISKELELLDRELCQLLLEGLEGVLRDQLALRALEEALEQGQSLGPVEPLDGPAGAVLECLVLSSGMLVPELAIPVVYLLGALTMLSETQHKLLAEALESQTLLGPLELVGSLLEQSAPWQERSTMSLPPGLLGNSWGEGAPAWVLLDECGLELGEDTPHVCWEPQAQGRMCALYASLALLSGLSQEPH

• Full Name: gasdermin D
• Calculated Molecular Weight: 484 aa, 53 kDa
• Observed Molecular Weight: 50-53 kDa
• GenBank Accession Number: BC008904
• Gene Symbol: GSDMD
• Gene ID (NCBI): 79792
• RRID: AB_10696319
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P57764
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GSDMD is a 53 kDa cytosolic protein and a member of the gasdermin protein family, which features 6 members in humans (GSDMA, -B, -C, -D, -E and -F. GSDMD was identified as the executioner of inflammasome-associated pyroptosis. Caspase-1, -11, or -4 cleave GSDMD after an aspartate residue within a central linker domain (D275 in humans and D276 in mice) to generate a 31-kDa N-terminal GSDMD fragment (GSDMD-NT) and a 22-kDa C-terminal fragment (GSDMD-CT). In addition to the canonical 31/22 kDa cleavage products, a 43 kDa fragment has been observed under specific experimental or pathological conditions. The generation of the 43 kDa fragment was observed upon caspase-3 and -7 activation during apoptosis. This antibody can only detect full-length GSDMD.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cell; Type III interferons induce pyroptosis in gut epithelial cells and impair mucosal repair; Authors - Kautilya K Jena
human	WB	Nature; Inflammasome-activated gasdermin D causes pyroptosis by forming membrane pores.; Authors - Xing Liu ; KD Validated
human	WB	ACS Nano; Engineering Extracellular Vesicles Restore the Impaired Cellular Uptake and Attenuate Intervertebral Disc Degeneration.; Authors - Zhiwei Liao
human	WB	Acta Pharm Sin B; Miltirone induces cell death in hepatocellular carcinoma cell through GSDME-dependent pyroptosis.; Authors - Xiaowei Zhang
human	WB	Acta Pharm Sin B; A high-throughput Gaussia luciferase reporter assay for screening potential gasdermin E activators against pancreatic cancer; Authors - Yang Liu
human	WB	Autophagy; Inhibition of PINK1 senses ROS signaling to facilitate neuroblastoma cell pyroptosis; Authors - Yuyuan Zhu ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Isha (Verified Customer) (03-02-2021)

Bands were so clear. Could not refrain myself from writing this review.

• Applications: Western Blot, Immunohistochemistry
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mice kidney