Tested Applications
| Positive WB detected in | A431 cells, Jurkat cells, THP-1 cells, AC16 cells |
| Positive IP detected in | THP-1 cells |
| Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, A431 cells, PC-3 cells |
| Positive FC (Intra) detected in | Jurkat cells |
This antibody can only dectect full length GSDMD.
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 ul suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20770-1-AP targets GSDMD in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, rabbit, chicken |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag14757 Product name: Recombinant human GSDMD protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 135-484 aa of BC008904 Sequence: HERHLRQPEHKVLQQLRSRGDNVYVVTEVLQTQKEVEVTRTHKREGSGRFSLPGATCLQGEGQGHLSQKKTVTIPSGSTLAFRVAQLVIDSDLDVLLFPDKKQRTFQPPATGHKRSTSEGAWPQLPSGLSMMRCLHNFLTDGVPAEGAFTEDFQGLRAEVETISKELELLDRELCQLLLEGLEGVLRDQLALRALEEALEQGQSLGPVEPLDGPAGAVLECLVLSSGMLVPELAIPVVYLLGALTMLSETQHKLLAEALESQTLLGPLELVGSLLEQSAPWQERSTMSLPPGLLGNSWGEGAPAWVLLDECGLELGEDTPHVCWEPQAQGRMCALYASLALLSGLSQEPH Predict reactive species |
| Full Name | gasdermin D |
| Calculated Molecular Weight | 484 aa, 53 kDa |
| Observed Molecular Weight | 50-53 kDa |
| GenBank Accession Number | BC008904 |
| Gene Symbol | GSDMD |
| Gene ID (NCBI) | 79792 |
| RRID | AB_10696319 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P57764 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GSDMD is a 53 kDa cytosolic protein and a member of the gasdermin protein family, which features 6 members in humans (GSDMA, -B, -C, -D, -E and -F. GSDMD was identified as the executioner of inflammasome-associated pyroptosis. Caspase-1, -11, or -4 cleave GSDMD after an aspartate residue within a central linker domain (D275 in humans and D276 in mice) to generate a 31-kDa N-terminal GSDMD fragment (GSDMD-NT) and a 22-kDa C-terminal fragment (GSDMD-CT). In addition to the canonical 31/22 kDa cleavage products, a 43 kDa fragment has been observed under specific experimental or pathological conditions. The generation of the 43 kDa fragment was observed upon caspase-3 and -7 activation during apoptosis. This antibody can only detect full-length GSDMD.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for GSDMD antibody 20770-1-AP | Download protocol |
| IF protocol for GSDMD antibody 20770-1-AP | Download protocol |
| IHC protocol for GSDMD antibody 20770-1-AP | Download protocol |
| IP protocol for GSDMD antibody 20770-1-AP | Download protocol |
| WB protocol for GSDMD antibody 20770-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GSDMD Polyclonal antibody (Cat# 20770-1-AP) has 612 citations. These appear in high-impact journals including Nature, Cell, Cell Research, Nature Cell Biology, Nature Communications, Nature Cancer, Science Advances, Molecular Cell, Journal of Experimental Medicine, Cell Reports, Cell Death & Differentiation, Cell Death & Disease, Frontiers in Immunology, Free Radical Biology & Medicine, Phytomedicine, and Biomaterials. Research fields span pyroptosis, inflammasome (NLRP3/AIM2) activation, PANoptosis, ferroptosis, cancer, neuroinflammation, and cardiovascular disease.
| Species | Application | Title |
|---|---|---|
Nature Inflammasome-activated gasdermin D causes pyroptosis by forming membrane pores.
| ||
Cell Type III interferons induce pyroptosis in gut epithelial cells and impair mucosal repair | ||
Cell Res Gasdermin D is an executor of pyroptosis and required for interleukin-1β secretion. | ||
Adv Mater Efficient Delivery of Lomitapide Using Hybrid Membrane-Coated Tetrahedral DNA Nanostructures for Glioblastoma Therapy | ||
Cell Mol Immunol NLRP3-dependent pyroptosis is required for HIV-1 gp120-induced neuropathology. |
Reviews
What customers say
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The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Mounika (Verified Customer) (01-08-2026) | Antibodies worked excellently for western blots and IHC, we also got the grant for this research project ! Amazing!
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Mounika (Verified Customer) (01-08-2026) | Mostly used for Western blots, bands are very excellent
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Isha (Verified Customer) (03-02-2021) | Bands were so clear. Could not refrain myself from writing this review.
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