Phospho-GSK3B (Ser9) Monoclonal antibody
Phospho-GSK3B (Ser9) Monoclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human and More (3)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
CloneNo.
1C9E2
Cat no : 67558-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Calyculin A treated PC-3 cells, Calyculin A treated HEK-293T cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | Calyculin A treated PC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 30 publications below |
| IHC | See 1 publications below |
| IF | See 3 publications below |
Product Information
67558-1-Ig targets Phospho-GSK3B (Ser9) in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Peptide |
| Full Name | glycogen synthase kinase 3 beta |
| Observed molecular weight | 48 kDa |
| GenBank accession number | NM_002093 |
| Gene symbol | GSK3B |
| Gene ID (NCBI) | 2932 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
Background Information
Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase .GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.In skeletal muscle, it contributes to INS regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. Researches showed that the crystal structure of human GSK3B, expressed in insect cells, at 2.8-angstrom resolution .
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-GSK3B (Ser9) antibody 67558-1-Ig | Download protocol |
| IHC protocol for Phospho-GSK3B (Ser9) antibody 67558-1-Ig | Download protocol |
| IF protocol for Phospho-GSK3B (Ser9) antibody 67558-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Stem Cell Res Ther Psoralen alleviates radiation-induced bone injury by rescuing skeletal stem cell stemness through AKT-mediated upregulation of GSK-3β and NRF2. | ||
Int J Biol Sci CCDC65, a Gene Knockout that leads to Early Death of Mice, acts as a potentially Novel Tumor Suppressor in Lung Adenocarcinoma. | ||
Nutrients Role of Epigallocatechin Gallate in Glucose, Lipid, and Protein Metabolism and L-Theanine in the Metabolism-Regulatory Effects of Epigallocatechin Gallate. | ||
Biomed Pharmacother Si-Miao-Yong-An Decoction attenuates isoprenaline-induced myocardial fibrosis in AMPK-driven Akt/mTOR and TGF-β/SMAD3 pathways. | ||
Biochim Biophys Acta Mol Basis Dis Deficiency of M-LP/Mpv17L leads to development of β-cell hyperplasia and improved glucose tolerance via activation of the Wnt and TGF-β pathways. | ||
Front Pharmacol Hsp47 Inhibitor Col003 Attenuates Collagen-Induced Platelet Activation and Cerebral Ischemic-Reperfusion Injury in Rats. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH X (Verified Customer) (01-18-2021) | It is a good Ab to detect GSK3b-pS9 in WB with mouse brain lysate
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