Phospho-GSK3B (Ser9) Monoclonal antibody

Phospho-GSK3B (Ser9) Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1


Human and More (3)







Cat no : 67558-1-Ig


GSK 3 beta, GSK3A B, GSK3B, GSK3B 216P, GSK3B S9, GSK3B Ser9, gsk3beta, Phospho-GSK3B (Ser9), Ser9

Tested Applications

Positive WB detected inCalyculin A treated PC-3 cells, Calyculin A treated HEK-293T cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inCalyculin A treated PC-3 cells
Planning an IF experiment? We recommend our CoraLite®488, CoraLite®594 and CoraLite®650 conjugated versions of this antibody.

Recommended dilution

Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

67558-1-Ig targets Phospho-GSK3B (Ser9) in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name glycogen synthase kinase 3 beta
Observed molecular weight 48 kDa
GenBank accession numberNM_002093
Gene symbol GSK3B
Gene ID (NCBI) 2932
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase .GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.In skeletal muscle, it contributes to INS regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. Researches showed that the crystal structure of human GSK3B, expressed in insect cells, at 2.8-angstrom resolution .


Product Specific Protocols
WB protocol for Phospho-GSK3B (Ser9) antibody 67558-1-IgDownload protocol
IHC protocol for Phospho-GSK3B (Ser9) antibody 67558-1-IgDownload protocol
IF protocol for Phospho-GSK3B (Ser9) antibody 67558-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Biomed Res Int

Heme Oxygenase-1 Suppresses Wnt Signaling Pathway in Nonalcoholic Steatohepatitis-Related Liver Fibrosis.

Authors - Jinghua Du

Cell Death Dis

Oxidative stress-induced mitophagy is suppressed by the miR-106b-93-25 cluster in a protective manner.

Authors - Cheng Zhang

Oxid Med Cell Longev

Apelin/APJ-Manipulated CaMKK/AMPK/GSK3β Signaling Works as an Endogenous Counterinjury Mechanism in Promoting the Vitality of Random-Pattern Skin Flaps.

Authors - Zhi-Ling Lou

Oxid Med Cell Longev

Inhibition of Growth of Colon Tumors and Proliferation of HT-29 Cells by Warburgia ugandensis Extract through Mediating G0/G1 Cell Cycle Arrest, Cell Apoptosis, and Intracellular ROS Generation.

Authors - Yongli Zhang

J Recept Signal Transduct Res

GLP-1 agonist liraglutide improves ouabain-induced mania and depressive state via GSK-3β pathway.

Authors - Mustafa Nusret Çiçekli

Exp Ther Med

Insulin and liraglutide attenuate brain pathology in diabetic mice by enhancing the Wnt/β-catenin signaling pathway.

Authors - Yuan Zhao


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


X (Verified Customer) (01-18-2021)

It is a good Ab to detect GSK3b-pS9 in WB with mouse brain lysate

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: mouse brain
Phospho-GSK3B (Ser9) Antibody Western Blot validation (1:2000 dilution) in mouse brain (Cat no:67558-1-Ig)