Phospho-GSK3B (Ser9) Monoclonal antibody

Phospho-GSK3B (Ser9) Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (3)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1C9E2

Cat no : 67558-1-Ig

Synonyms

GSK 3 beta, GSK3A B, GSK3B, GSK3B 216P, GSK3B S9, GSK3B Ser9, gsk3beta, Phospho-GSK3B (Ser9), Ser9



Tested Applications

Positive WB detected inCalyculin A treated PC-3 cells, Calyculin A treated HEK-293T cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inCalyculin A treated PC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

67558-1-Ig targets Phospho-GSK3B (Ser9) in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name glycogen synthase kinase 3 beta
Observed molecular weight 48 kDa
GenBank accession numberNM_002093
Gene symbol GSK3B
Gene ID (NCBI) 2932
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase .GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.In skeletal muscle, it contributes to INS regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. Researches showed that the crystal structure of human GSK3B, expressed in insect cells, at 2.8-angstrom resolution .

Protocols

Product Specific Protocols
WB protocol for Phospho-GSK3B (Ser9) antibody 67558-1-IgDownload protocol
IHC protocol for Phospho-GSK3B (Ser9) antibody 67558-1-IgDownload protocol
IF protocol for Phospho-GSK3B (Ser9) antibody 67558-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Stem Cell Res Ther

Psoralen alleviates radiation-induced bone injury by rescuing skeletal stem cell stemness through AKT-mediated upregulation of GSK-3β and NRF2.

Authors - Bo-Feng Yin
humanWB

Int J Biol Sci

CCDC65, a Gene Knockout that leads to Early Death of Mice, acts as a potentially Novel Tumor Suppressor in Lung Adenocarcinoma.

Authors - Ziyan Zhang
ratWB

Nutrients

Role of Epigallocatechin Gallate in Glucose, Lipid, and Protein Metabolism and L-Theanine in the Metabolism-Regulatory Effects of Epigallocatechin Gallate.

Authors - Ling Lin
ratWB

Biomed Pharmacother

Si-Miao-Yong-An Decoction attenuates isoprenaline-induced myocardial fibrosis in AMPK-driven Akt/mTOR and TGF-β/SMAD3 pathways.

Authors - Yuqian Zhao
mouseWB,IF

Biochim Biophys Acta Mol Basis Dis

Deficiency of M-LP/Mpv17L leads to development of β-cell hyperplasia and improved glucose tolerance via activation of the Wnt and TGF-β pathways.

Authors - Reiko Iida
ratWB

Front Pharmacol

Hsp47 Inhibitor Col003 Attenuates Collagen-Induced Platelet Activation and Cerebral Ischemic-Reperfusion Injury in Rats.

Authors - Shuang Wu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

X (Verified Customer) (01-18-2021)

It is a good Ab to detect GSK3b-pS9 in WB with mouse brain lysate

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: mouse brain
Phospho-GSK3B (Ser9) Antibody Western Blot validation (1:2000 dilution) in mouse brain (Cat no:67558-1-Ig)