|Positive WB detected in||HeLa cells, Calyculin A treated PC-3 cells, Calyculin A treated HEK-293T cells|
|Positive IHC detected in||human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||Calyculin A treated PC-3 cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunohistochemistry (IHC)||IHC : 1:150-1:600|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
67558-1-Ig targets Phospho-GSK3B (Ser9) in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Mouse / IgG1|
|Full Name||glycogen synthase kinase 3 beta|
|Observed molecular weight||48 kDa|
|GenBank accession number||NM_002093|
|Gene ID (NCBI)||2932|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase .GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.In skeletal muscle, it contributes to INS regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. Researches showed that the crystal structure of human GSK3B, expressed in insect cells, at 2.8-angstrom resolution .
|Product Specific Protocols|
|WB protocol for Phospho-GSK3B (Ser9) antibody 67558-1-Ig||Download protocol|
|IHC protocol for Phospho-GSK3B (Ser9) antibody 67558-1-Ig||Download protocol|
|IF protocol for Phospho-GSK3B (Ser9) antibody 67558-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
X (Verified Customer) (01-18-2021)
It is a good Ab to detect GSK3b-pS9 in WB with mouse brain lysate