Tested Applications
| Positive WB detected in | HeLa cells, Calyculin A treated PC-3 cells, Calyculin A treated HEK-293T cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Calyculin A treated PC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67558-1-Ig targets Phospho-GSK3B (Ser9) in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat, pig, chicken, bovine |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | glycogen synthase kinase 3 beta |
| Observed Molecular Weight | 48 kDa |
| GenBank Accession Number | NM_002093 |
| Gene Symbol | GSK3B |
| Gene ID (NCBI) | 2932 |
| RRID | AB_2882772 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P49841 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase .GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.In skeletal muscle, it contributes to INS regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. Researches showed that the crystal structure of human GSK3B, expressed in insect cells, at 2.8-angstrom resolution .
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Phospho-GSK3B (Ser9) antibody 67558-1-Ig | Download protocol |
| IHC protocol for Phospho-GSK3B (Ser9) antibody 67558-1-Ig | Download protocol |
| WB protocol for Phospho-GSK3B (Ser9) antibody 67558-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Phospho-GSK3B (Ser9) Monoclonal antibody (Cat# 67558-1-Ig) has 169 citations. These appear in journals including Signal Transduction and Targeted Therapy, Cell Metabolism, Advanced Materials, Autophagy, Cell Death & Differentiation, Cancer Letters, International Journal of Biological Sciences, Phytomedicine, Molecular Psychiatry, Journal of Translational Medicine, Frontiers in Pharmacology, and Bioorganic Chemistry, among others. Research fields span oncology (colorectal, liver, lung, breast, gastric cancers), Alzheimer's disease and neurodegeneration, diabetes, fibrosis, and Wnt/β-catenin and PI3K/AKT/GSK3β signaling pathways.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Gut dysbiosis conveys psychological stress to activate LRP5/β-catenin pathway promoting cancer stemness | ||
Cell Metab Nicotinamide metabolism face-off between macrophages and fibroblasts manipulates the microenvironment in gastric cancer | ||
Adv Mater Noninvasive Optogenetics Realized by iPSC-Derived Tentacled Carrier in Alzheimer's Disease Treatment | ||
Bioact Mater Self-assembling peptide inspired by insulin and type 1 insulin-like growth factor for the treatment of androgenetic alopecia | ||
Autophagy WNT2B impairs endosomal trafficking via WASHC5 to inhibit autophagy: a novel non-secretory WNT pathway. | ||
J Adv Res Fatty acid-binding protein 5 aggravates pulmonary artery fibrosis in pulmonary hypertension secondary to left heart disease via activating wnt/β-catenin pathway |
Reviews
What customers say
All three reviews rated this antibody 5 stars. Users reported sharp, clear bands in Western Blot across multiple cell types and tissues, including MDA-MB-231 cells, iPS-derived cardiomyocytes/fibroblasts, and mouse brain lysate. One reviewer also confirmed strong green signal in Immunofluorescence. Recommended dilutions ranged from 1:1000 to 1:5000 for WB and 1:500 for IF. Overall, reviewers highlighted reliable detection of GSK3β-pS9 with consistent performance across applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Md Asad (Verified Customer) (05-07-2026) | Sharp bands in my western blot.
|
Asim (Verified Customer) (12-12-2025) | Good WB bands and IF signals (Green) with this antibody.
 |
X (Verified Customer) (01-18-2021) | It is a good Ab to detect GSK3b-pS9 in WB with mouse brain lysate
 |
