Phospho-GSK3B (Ser9) Monoclonal antibody

Phospho-GSK3B (Ser9) Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1


Human and More (4)







Cat no : 67558-1-Ig


GSK 3 beta, GSK3A B, GSK3B, GSK3B 216P, GSK3B S9, GSK3B Ser9, gsk3beta, Phospho-GSK3B (Ser9), Ser9

Tested Applications

Positive WB detected inHeLa cells, Calyculin A treated PC-3 cells, Calyculin A treated HEK-293T cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inCalyculin A treated PC-3 cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67558-1-Ig targets Phospho-GSK3B (Ser9) in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman, mouse, rat, bovine
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name glycogen synthase kinase 3 beta
Observed Molecular Weight 48 kDa
GenBank Accession NumberNM_002093
Gene Symbol GSK3B
Gene ID (NCBI) 2932
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase .GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.In skeletal muscle, it contributes to INS regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. Researches showed that the crystal structure of human GSK3B, expressed in insect cells, at 2.8-angstrom resolution .


Product Specific Protocols
WB protocol for Phospho-GSK3B (Ser9) antibody 67558-1-IgDownload protocol
IHC protocol for Phospho-GSK3B (Ser9) antibody 67558-1-IgDownload protocol
IF protocol for Phospho-GSK3B (Ser9) antibody 67558-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols




Combinational application of the natural products 1-deoxynojirimycin and morin ameliorates insulin resistance and lipid accumulation in prediabetic mice

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Int J Mol Sci

Neuroprotection and Mechanism of Gas-miR36-5p from Gastrodia elata in an Alzheimer's Disease Model by Regulating Glycogen Synthase Kinase-3β

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J Ethnopharmacol

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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


X (Verified Customer) (01-18-2021)

It is a good Ab to detect GSK3b-pS9 in WB with mouse brain lysate

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: mouse brain
Phospho-GSK3B (Ser9) Antibody Western Blot validation (1:2000 dilution) in mouse brain (Cat no:67558-1-Ig)