CoraLite®488-conjugated Phospho-GSK3B (Ser9) Monoclonal antibody

Phospho-GSK3B (Ser9) Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF, FC (Intra)

Conjugate

CoraLite®488 Fluorescent Dye

CloneNo.

1C9E2

Cat no : CL488-67558

Synonyms

GSK 3 beta, GSK3A B, GSK3B, GSK3B 216P, gsk3beta, Phospho-GSK3B (Ser9)



Tested Applications

Positive IF detected inCalyculin A treated PC-3 cells
Positive FC detected inCalyculin A treated PC-3 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-67558 targets Phospho-GSK3B (Ser9) in IF, FC (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name glycogen synthase kinase 3 beta
Observed molecular weight 48 kDa
GenBank accession numberNM_002093
Gene symbol GSK3B
Gene ID (NCBI) 2932
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission maxima wavelengths488 nm / 515 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase .GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.In skeletal muscle, it contributes to INS regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. Researches showed that the crystal structure of human GSK3B, expressed in insect cells, at 2.8-angstrom resolution .

Protocols

Product Specific Protocols
IF protocol for CL488 Phospho-GSK3B (Ser9) antibody CL488-67558Download protocol
Standard Protocols
Click here to view our Standard Protocols