CoraLite® Plus 488-conjugated Phospho-GSK3B (Ser9) Monoclonal antibody

Phospho-GSK3B (Ser9) Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1C9E2

Cat no : CL488-67558

Synonyms

GSK 3 beta, GSK3A B, GSK3B, GSK3B 216P, gsk3beta, Phospho-GSK3B (Ser9)

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Validation Data Gallery

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  IF Staining of PC-3 using CL488-67558

  Immunofluorescent analysis of (4% PFA) fixed Calyculin A treated PC-3 cells and non-treated PC-3 cells using CoraLite® Plus 488 Phospho-GSK3B (Ser9) antibody (CL488-67558, Clone: 1C9E2 ) at dilution of 1:100.

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  FC experiment of PC-3 using CL488-67558

  1X10^6 PC-3 cells untreated (dashed lines) or treated with Calyculin A (red) were intracellularly stained with 0.13 ug CoraLite® Plus 488 Anti-Human Phospho-GSK3B (Ser9) (CL488-67558, Clone:1C9E2), or 0.13 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

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Tested Applications

• Positive IF detected in: Calyculin A treated PC-3 cells
• Positive FC detected in: Calyculin A treated PC-3 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.13 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67558 targets Phospho-GSK3B (Ser9) in IF, FC (Intra) applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: glycogen synthase kinase 3 beta
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: NM_002093
• Gene Symbol: GSK3B
• Gene ID (NCBI): 2932
• RRID: AB_2919500
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase .GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.In skeletal muscle, it contributes to INS regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. Researches showed that the crystal structure of human GSK3B, expressed in insect cells, at 2.8-angstrom resolution .