ChromoTek GST-Trap Agarose
GST-Trap is an affinity resin for IP of GST fusion proteins. It consists of a GST Nanobody/ VHH coupled to agarose beads.
Specificity
Glutathione S-Transferase (GST)
Applications
IP, CoIP, ChIP, RIP
Conjugate
Agarose
Cat no : sta
Synonyms
Validation Data Gallery
Product Information
GST-Trap is an affinity resin for IP of GST fusion proteins. It consists of a GST Nanobody/ VHH coupled to agarose beads.
| Applications | IP, CoIP, ChIP, RIP |
| Clonality | Monoclonal |
| Host | Alpaca |
| Affinity | Dissociation constant KD of 1 nM |
| Specificity/Target | Glutathione S-Transferase (GST) from Schistosoma japonicum |
| Coupled VHH | Recombinant, monoclonal anti-GST single domain antibody (sdAb) fragment |
| Conjugate | Unconjugated |
| Beads | ~ 90 µm (cross-linked 4 % agarose beads) |
| Elution buffer | SDS sample buffer for SDS/PAGE & Western blot 0.2 M glycine pH 2.5 |
| Storage Buffer | 20% EtOH |
| Storage Condition | Upon receipt store at +4°C. Do not freeze! |
Documentation
| SDS |
|---|
| SDS GST-Trap Agarose (PDF) |
| Protocol |
|---|
| Protocol GST-Trap Agarose (PDF) |
| Whitepaper |
|---|
| Immunoprecipitation of GST- and MBP-fusion proteins (PDF) |
| Brochure |
|---|
| Product brochure GST-Trap (PDF) |
| Trouble shooting |
|---|
| Troubleshooting guide immunoprecipitation (PDF) |
Publications
| Application | Title |
|---|---|
Antibodies (Basel) HDX-MS for Epitope Characterization of a Therapeutic ANTIBODY Candidate on the Calcium-Binding Protein Annexin-A1. | |
Turk J Biol Keratin 14 is a novel interaction partner of keratinocyte differentiation regulator: receptor-interacting protein kinase 4. | |
Virology Bovine herpesvirus type-1 glycoprotein K (gK) interacts with UL20 and is required for infectious virus production. | |
Eur J Cell Biol Acetylation of translationally controlled tumor protein promotes its degradation through chaperone-mediated autophagy. | |
J Proteomics Towards multiplexed protein-protein interaction analysis using protein tag-specific nanobodies. | |