ChromoTek GST-Trap Agarose

GST-Trap is an affinity resin for IP of GST fusion proteins. It consists of a GST Nanobody/ VHH coupled to agarose beads.

Ready to use reagent

Specificity

Glutathione S-Transferase (GST)

Applications

IP, CoIP, ChIP, RIP

Conjugate

Agarose

Publications

9

Cat no : sta

Synonyms

GST-Trap, GST, GST tag, Glutathione S transferase

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Validation Data Gallery

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  Pull-down of GST with GST-Trap Agarose: Coomassie and Western blot; Immunoprecipitation of GST with GST-Trap. Note effective pull-down of GST-fusion proteins: No GST-fusions left in non-bound (FT) lane in Western Blot. I: Input, FT: Flow-Through, B: Bound.

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Product Information

GST-Trap is an affinity resin for IP of GST fusion proteins. It consists of a GST Nanobody/ VHH coupled to agarose beads.

• Applications: IP, CoIP, ChIP, RIP
• Clonality: Monoclonal
• Host: Alpaca
• Affinity: Dissociation constant KD of 1 nM
• Specificity/Target: Glutathione S-Transferase (GST) from Schistosoma japonicum
• Coupled VHH: Recombinant, monoclonal anti-GST single domain antibody (sdAb) fragment
• Conjugate: Agarose
• Beads: ~ 90 µm (cross-linked 4 % agarose beads)
• Elution buffer: SDS sample buffer for SDS/PAGE & Western blot; 0.2 M glycine pH 2.5
• Storage Buffer: 20% EtOH
• Storage Condition: Upon receipt store at +4°C. Do not freeze!

Publications

Application	Title
IP	Turk J Biol; Keratin 14 is a novel interaction partner of keratinocyte differentiation regulator: receptor-interacting protein kinase 4.; Authors - Ceren Sümer
IP	Virology; Bovine herpesvirus type-1 glycoprotein K (gK) interacts with UL20 and is required for infectious virus production.; Authors - Muzammel Haque
IP	Eur J Cell Biol; Acetylation of translationally controlled tumor protein promotes its degradation through chaperone-mediated autophagy.; Authors - Anne Bonhoure
IP	J Proteomics; Towards multiplexed protein-protein interaction analysis using protein tag-specific nanobodies.; Authors - Nicola Groll
IP	J Virol; The Kaposi's Sarcoma-Associated Herpesvirus ORF34 Protein Interacts and Stabilizes HIF-2α via Binding to the HIF-2α bHLH and PAS Domains.; Authors - Muzammel Haque
IP	J Cell Biol; ACBD5 and VAPB mediate membrane associations between peroxisomes and the ER.; Authors - Joseph L Costello