ChromoTek GST-Trap Agarose

Validation Data Gallery

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  Pull-down of GST with GST-Trap Agarose: Coomassie and Western blot; Immunoprecipitation of GST with GST-Trap. Note effective pull-down of GST-fusion proteins: No GST-fusions left in non-bound (FT) lane in Western Blot. I: Input, FT: Flow-Through, B: Bound.

GST-Trap is an affinity resin for IP of GST fusion proteins. It consists of a GST Nanobody/ VHH coupled to agarose beads.

Cat No. sta

Publications(18)

Specificity

GST

Applications

IP, CoIP, ChIP, RIP

Type

Nanobody

Conjugate

Agarose

Synonyms

GST-Trap, GST, GST tag, Glutathione S transferase

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Product Information

GST-Trap is an affinity resin for IP of GST fusion proteins. It consists of a GST Nanobody/ VHH coupled to agarose beads.

• Description: Immunoprecipitation of GST-fusion proteins and their interacting factors with anti-GST Nanobody conjugated to beads.

  • Effectively immunoprecipitates low expressed GST-fusion proteins

  • Considerably higher affinity than glutathione cellulose and other affinity resins

  • Strong binding even under harsh washing conditions

  • No heavy & light chains in your downstream application

• Applications: IP, CoIP, ChIP, RIP
• Specificity/Target: Glutathione S-Transferase (GST) from Schistosoma japonicum
• Binding capacity: 7.5-10 μg of recombinant GST per 25 μL bead slurry
• Conjugate: Agarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)
• Elution buffer: SDS sample buffer; 0.2 M glycine pH 2.5
• Wash buffer compatibility: 4 M urea, 2 M NaCl, 10 mM DTT, 2 % Nonidet P40 Substitute, 1 % Triton X-100
• Type: Nanobody
• Class: Recombinant
• Host: Alpaca
• Affinity (KD): Dissociation constant K_D of 1 nM
• Compatibility with mass spectrometry: The GST-Trap is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry
• RRID: AB_2631414
• Storage Buffer: 20% ethanol
• Storage Condition: Shipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze!
• Size: 25ul/reactions (eg：20rxns=500ul slurry)

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Publications

Application	Title
IP	Plant Biotechnol J; Wheat yellow mosaic virus NIb targets TaVTC2 to elicit broad-spectrum pathogen resistance in wheat; Authors - Tianye Zhang
IP	Nat Commun; Dephosphorylation of the HIV-1 restriction factor SAMHD1 is mediated by PP2A-B55α holoenzymes during mitotic exit.; Authors - Kerstin Schott
IP	EMBO J; The Arabidopsis Cdk1/Cdk2 homolog CDKA;1 controls chromosome axis assembly during plant meiosis.; Authors - Chao Yang
IP	Curr Biol; Aurora B-INCENP Localization at Centromeres/Inner Kinetochores Is Required for Chromosome Bi-orientation in Budding Yeast.; Authors - Luis J García-Rodríguez
IP	J Cell Biol; ACBD5 and VAPB mediate membrane associations between peroxisomes and the ER.; Authors - Joseph L Costello
IP	J Cell Biol; Regulating peroxisome-ER contacts via the ACBD5-VAPB tether by FFAT motif phosphorylation and GSK3β.; Authors - Suzan Kors