ChromoTek GST-Trap Agarose

GST-Trap is an affinity resin for IP of GST fusion proteins. It consists of a GST Nanobody/ VHH coupled to agarose beads.

Specificity

Glutathione S-Transferase (GST)

Applications

IP, CoIP, ChIP, RIP

Conjugate

Agarose

Cat no : sta

Synonyms

GST-Trap, GST, GST tag, Glutathione S transferase



Product Information

GST-Trap is an affinity resin for IP of GST fusion proteins. It consists of a GST Nanobody/ VHH coupled to agarose beads.

ApplicationsIP, CoIP, ChIP, RIP
ClonalityMonoclonal
HostAlpaca
AffinityDissociation constant KD of 1 nM
Specificity/TargetGlutathione S-Transferase (GST) from Schistosoma japonicum
Coupled VHHRecombinant, monoclonal anti-GST single domain antibody (sdAb) fragment
ConjugateAgarose
Beads~ 90 µm (cross-linked 4 % agarose beads)
Elution buffer SDS sample buffer for SDS/PAGE & Western blot
0.2 M glycine pH 2.5
Storage Buffer20% EtOH
Storage ConditionUpon receipt store at +4°C. Do not freeze!

Publications

ApplicationTitle
IP

Antibodies (Basel)

HDX-MS for Epitope Characterization of a Therapeutic ANTIBODY Candidate on the Calcium-Binding Protein Annexin-A1.

Authors - Marius Gramlich
IP

Mol Plant Pathol

Binding between elongation factor 1A and the 3'-UTR of Chinese wheat mosaic virus is crucial for virus infection.

Authors - Xuan Chen
IP

Turk J Biol

Keratin 14 is a novel interaction partner of keratinocyte differentiation regulator: receptor-interacting protein kinase 4.

Authors - Ceren Sümer
IP

Virology

Bovine herpesvirus type-1 glycoprotein K (gK) interacts with UL20 and is required for infectious virus production.

Authors - Muzammel Haque
IP

Eur J Cell Biol

Acetylation of translationally controlled tumor protein promotes its degradation through chaperone-mediated autophagy.

Authors - Anne Bonhoure
IP

J Proteomics

Towards multiplexed protein-protein interaction analysis using protein tag-specific nanobodies.

Authors - Nicola Groll