ChromoTek GST-Trap Agarose
GST-Trap is an affinity resin for IP of GST fusion proteins. It consists of a GST Nanobody/ VHH coupled to agarose beads.
Ready to use reagent
Specificity
Glutathione S-Transferase (GST)
Applications
IP, CoIP, ChIP, RIP
Conjugate
Agarose
Cat no : sta
Synonyms
Validation Data Gallery
Product Information
GST-Trap is an affinity resin for IP of GST fusion proteins. It consists of a GST Nanobody/ VHH coupled to agarose beads.
Applications | IP, CoIP, ChIP, RIP |
Clonality | Monoclonal |
Host | Alpaca |
Affinity | Dissociation constant KD of 1 nM |
Specificity/Target | Glutathione S-Transferase (GST) from Schistosoma japonicum |
Coupled VHH | Recombinant, monoclonal anti-GST single domain antibody (sdAb) fragment |
Conjugate | Agarose |
Beads | ~ 90 µm (cross-linked 4 % agarose beads) |
Elution buffer | SDS sample buffer for SDS/PAGE & Western blot 0.2 M glycine pH 2.5 |
Storage Buffer | 20% EtOH |
Storage Condition | Upon receipt store at +4°C. Do not freeze! |
Documentation
SDS |
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SDS GST-Trap Agarose (PDF) |
Protocol |
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Protocol GST-Trap Agarose (PDF) |
Brochure |
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Product brochure GST-Trap (PDF) |
Trouble shooting |
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Troubleshooting guide immunoprecipitation (PDF) |
Whitepaper |
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Immunoprecipitation of GST- and MBP-fusion proteins (PDF) |
Publications
Application | Title |
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Turk J Biol Keratin 14 is a novel interaction partner of keratinocyte differentiation regulator: receptor-interacting protein kinase 4. | |
Virology Bovine herpesvirus type-1 glycoprotein K (gK) interacts with UL20 and is required for infectious virus production. | |
Eur J Cell Biol Acetylation of translationally controlled tumor protein promotes its degradation through chaperone-mediated autophagy. | |
J Proteomics Towards multiplexed protein-protein interaction analysis using protein tag-specific nanobodies. | |
J Virol The Kaposi's Sarcoma-Associated Herpesvirus ORF34 Protein Interacts and Stabilizes HIF-2α via Binding to the HIF-2α bHLH and PAS Domains. | |