• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0

ChromoTek GST-Trap Agarose

GST-Trap is an affinity resin for IP of GST fusion proteins. It consists of a GST Nanobody/ VHH coupled to agarose beads.

Specificity

Glutathione S-Transferase (GST)

Applications

IP, CoIP, ChIP, RIP

Conjugate

Agarose

Cat no : sta

Synonyms

GST-Trap, GST, GST tag, Glutathione S transferase

Validation Data Gallery

  • Pull-down of GST with GST-Trap Agarose: Coomassie and Western blot; Immunoprecipitation of GST with GST-Trap. Note effective pull-down of GST-fusion proteins: No GST-fusions left in non-bound (FT) lane in Western Blot. I: Input, FT: Flow-Through, B: Bound.

    Pull-down of GST with GST-Trap Agarose: Coomassie and Western blot; Immunoprecipitation of GST with GST-Trap. Note effective pull-down of GST-fusion proteins: No GST-fusions left in non-bound (FT) lane in Western Blot. I: Input, FT: Flow-Through, B: Bound.

Product Information

GST-Trap is an affinity resin for IP of GST fusion proteins. It consists of a GST Nanobody/ VHH coupled to agarose beads.

ApplicationsIP, CoIP, ChIP, RIP
ClonalityMonoclonal
HostAlpaca
AffinityDissociation constant KD of 1 nM
Specificity/TargetGlutathione S-Transferase (GST) from Schistosoma japonicum
Coupled VHHRecombinant, monoclonal anti-GST single domain antibody (sdAb) fragment
ConjugateAgarose
Beads~ 90 µm (cross-linked 4 % agarose beads)
Elution buffer SDS sample buffer for SDS/PAGE & Western blot
0.2 M glycine pH 2.5
Storage Buffer20% EtOH
Storage ConditionUpon receipt store at +4°C. Do not freeze!

Publications

ApplicationTitle
IP

Antibodies (Basel)

HDX-MS for Epitope Characterization of a Therapeutic ANTIBODY Candidate on the Calcium-Binding Protein Annexin-A1.

Authors - Marius Gramlich
View Article
IP

Mol Plant Pathol

Binding between elongation factor 1A and the 3'-UTR of Chinese wheat mosaic virus is crucial for virus infection.

Authors - Xuan Chen
View Article
IP

Turk J Biol

Keratin 14 is a novel interaction partner of keratinocyte differentiation regulator: receptor-interacting protein kinase 4.

Authors - Ceren Sümer
View Article
IP

Virology

Bovine herpesvirus type-1 glycoprotein K (gK) interacts with UL20 and is required for infectious virus production.

Authors - Muzammel Haque
View Article
IP

Eur J Cell Biol

Acetylation of translationally controlled tumor protein promotes its degradation through chaperone-mediated autophagy.

Authors - Anne Bonhoure
View Article
IP

J Proteomics

Towards multiplexed protein-protein interaction analysis using protein tag-specific nanobodies.

Authors - Nicola Groll
View Article