ChromoTek GST-Trap Agarose, kit

GST-Trap is an affinity resin for IP of GST fusion proteins. It consists of a GST Nanobody/ VHH coupled to agarose beads.

Specificity

Glutathione S-Transferase (GST)

Applications

IP, CoIP, ChIP, RIP

Conjugate

Agarose

Cat no : stak

Synonyms

GST-Trap, GST, GST tag, Glutathione S transferase



Product Information

GST-Trap is an affinity resin for IP of GST fusion proteins. It consists of a GST Nanobody/ VHH coupled to agarose beads.

ApplicationsIP, CoIP, ChIP, RIP
Clonality VHH
HostAlpaca
AffinityDissociation constant KD of 1 nM
Specificity/TargetGlutathione S-Transferase (GST) from Schistosoma japonicum
Coupled VHHRecombinant, monoclonal anti-GST single domain antibody (sdAb) fragment
ConjugateAgarose
Beads~ 90 µm (cross-linked 4 % agarose beads)
Elution bufferSDS sample buffer for SDS/PAGE & Western blot
0.2 M glycine pH 2.5
Storage Buffer20% EtOH
Storage ConditionUpon receipt store at +4°C. Do not freeze!

Kit components

ComponentDescription
GST-Trap Agarose20 reactions (500 µL)
Lysis bufferOptimized for cytoplasmatic proteins and mammalian cell lysis
RIPA bufferOptimized for nuclear/chromatin proteins and mammalian cell lysis
Wash buffer Removal of unwanted proteins, peptides, etc.
Dilution bufferDilution of cell lysate
Elution bufferFor acidic elution