Tested Applications
| Positive WB detected in | mouse testis tissue, rat testis tissue, human testis tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16703-1-AP targets GSTA3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10101 Product name: Recombinant human GSTA3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-222 aa of BC020619 Sequence: MAGKPKLHYFNGRGRMEPIRWLLAAAGVEFEEKFIGSAEDLGKLRNDGSLMFQQVPMVEIDGIKLVQTRAILNYIASKYNLYGKDIKERALIDMYTEGMADLNEMILLLPLCRPEEKDAKIALIKEKTKSRYFPAFEKVLQSHGQDYLVGNKLSRADISLVELLYYVEELDSSLISNFPLLKALKTRISNLPTVKKFLQPGSPRKPPADAKALEEARKIFRF Predict reactive species |
| Full Name | glutathione S-transferase alpha 3 |
| Calculated Molecular Weight | 222 aa, 25 kDa |
| Observed Molecular Weight | 25 kDa |
| GenBank Accession Number | BC020619 |
| Gene Symbol | GSTA3 |
| Gene ID (NCBI) | 2940 |
| RRID | AB_2279523 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q16772 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for GSTA3 antibody 16703-1-AP | Download protocol |
| IHC protocol for GSTA3 antibody 16703-1-AP | Download protocol |
| IP protocol for GSTA3 antibody 16703-1-AP | Download protocol |
| WB protocol for GSTA3 antibody 16703-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GSTA3 Polyclonal antibody (Cat# 16703-1-AP) has 6 citations published in Radiotherapy and Oncology, Frontiers in Pharmacology, American Journal of Pathology, Age, PLoS One, and Journal of Toxicologic Pathology. Associated research fields include head and neck cancer radiochemotherapy, colitis and T-cell polarization, acetaminophen-induced liver injury, growth hormone signaling, cigarette-induced oxidative stress in lung epithelial cells, and thioacetamide-induced hepatocellular necrosis in obese mice.
| Species | Application | Title |
|---|---|---|
Radiother Oncol Transcriptomic and epigenetic landscape of nimorazole-enhanced radiochemotherapy in head and neck cancer | ||
Front Pharmacol PDE9 Inhibitor PF-04447943 Attenuates DSS-Induced Colitis by Suppressing Oxidative Stress, Inflammation, and Regulating T-Cell Polarization. | ||
Am J Pathol Novel Protective Role of Nicotinamide Phosphoribosyltransferase in Acetaminophen-Induced Acute Liver Injury in Mice. | ||
PLoS One Heat-Not-Burn cigarette induces oxidative stress response in primary rat alveolar epithelial cells. | ||
J Toxicol Pathol Thioacetamide-induced Hepatocellular Necrosis Is Attenuated in Diet-induced Obese Mice. |
