GSTO1 Monoclonal antibody

GSTO1 Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

5E1E10

Cat no : 60150-2-Ig

Synonyms

glutathione S transferase omega 1, GSTO 1, GSTO1



Tested Applications

Positive WB detected inhuman heart tissue, Jurkat cells, NIH/3T3 cells, Raji cells, ROS1728 cells, U-937 cells
Positive IHC detected inhuman oesophagus cancer tissue, human liver tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

60150-2-Ig targets GSTO1 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GSTO1 fusion protein Ag7199
Full Name glutathione S-transferase omega 1
Calculated molecular weight 28 kDa
Observed molecular weight 27-30 kDa
GenBank accession numberBC000127
Gene symbol GSTO1
Gene ID (NCBI) 9446
Conjugate Unconjugated
Form Liquid
Purification Method Caprylic acid/ammonium sulfate precipitation
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Glutathione-S-transferase omega 1 (GSTO1) belongs to a new subfamily of GSTs and is the rate limiting enzyme for biotransformation of inorganic arsenic, environmental carcinogen. Expression of GSTO1-1 was abundant in a wide range of normal tissues, particularly liver, macrophages, glial cells, and endocrine cells.

Protocols

Product Specific Protocols
WB protocol for GSTO1 antibody 60150-2-IgDownload protocol
IHC protocol for GSTO1 antibody 60150-2-IgDownload protocol
IF protocol for GSTO1 antibody 60150-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Toxicol Appl Pharmacol

Effects of Nrf2 deficiency on arsenic metabolism in mice.

Authors - Huihui Wang