Filter: AllWBIHCIF/ICC

WB analysis using 60150-2-Ig (same clone as 60150-2-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 60150-2-Ig (GSTO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60150-2-PBS in a different storage buffer formulation.
WB analysis of human heart using 60150-2-Ig (same clone as 60150-2-PBS)
human heart tissue were subjected to SDS PAGE followed by western blot with 60150-2-Ig (GSTO1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60150-2-PBS in a different storage buffer formulation.
WB analysis of U-937 using 60150-2-Ig (same clone as 60150-2-PBS)
U-937 cells were subjected to SDS PAGE followed by western blot with 60150-2-Ig (GSTO1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60150-2-PBS in a different storage buffer formulation.
WB analysis of Raji using 60150-2-Ig (same clone as 60150-2-PBS)
Raji cells were subjected to SDS PAGE followed by western blot with 60150-2-Ig (GSTO1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60150-2-PBS in a different storage buffer formulation.
WB analysis of ROS1728 using 60150-2-Ig (same clone as 60150-2-PBS)
ROS1728 cells were subjected to SDS PAGE followed by western blot with 60150-2-Ig (GSTO1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60150-2-PBS in a different storage buffer formulation.
WB analysis of NIH/3T3 using 60150-2-Ig (same clone as 60150-2-PBS)
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60150-2-Ig (GSTO1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60150-2-PBS in a different storage buffer formulation.
IHC staining of human oesophagus cancer using 60150-2-Ig (same clone as 60150-2-PBS)
Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60150-2-Ig (GSTO1 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60150-2-PBS in a different storage buffer formulation.
IHC staining of human oesophagus cancer using 60150-2-Ig (same clone as 60150-2-PBS)
Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60150-2-Ig (GSTO1 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60150-2-PBS in a different storage buffer formulation.
IHC staining of human liver using 60150-2-Ig (same clone as 60150-2-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 60150-2-Ig (GSTO1 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 60150-2-PBS in a different storage buffer formulation.
IHC staining of human liver using 60150-2-Ig (same clone as 60150-2-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 60150-2-Ig (GSTO1 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 60150-2-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 60150-2-Ig (same clone as 60150-2-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60150-2-Ig (GSTO1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60150-2-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 60150-2-Ig (same clone as 60150-2-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60150-2-Ig (GSTO1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60150-2-PBS in a different storage buffer formulation.
IF Staining of PC-3 using 60150-2-Ig (same clone as 60150-2-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using GSTO1 antibody (60150-2-Ig, Clone: 5E1E10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 60150-2-PBS in a different storage buffer formulation.

GSTO1 Monoclonal antibody, PBS Only

GSTO1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 60150-2-PBS

Clone No.5E1E10

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

EC:1.20.4.2, EC:1.8.5.1, EC:2.5.1.18, glutathione S transferase omega 1, Glutathione S-transferase omega 1-1

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

60150-2-PBS targets GSTO1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	GSTO1 fusion protein Ag7199 Predict reactive species
Full Name	glutathione S-transferase omega 1
Calculated Molecular Weight	28 kDa
Observed Molecular Weight	27-30 kDa
GenBank Accession Number	BC000127
Gene Symbol	GSTO1
Gene ID (NCBI)	9446
RRID	AB_10644447
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P78417
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Glutathione-S-transferase omega 1 (GSTO1) belongs to a new subfamily of GSTs and is the rate limiting enzyme for biotransformation of inorganic arsenic, environmental carcinogen. Expression of GSTO1-1 was abundant in a wide range of normal tissues, particularly liver, macrophages, glial cells, and endocrine cells.