Granzyme B Polyclonal antibody

Granzyme B Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13588-1-AP

Synonyms

CCPI, CGL 1, CGL1, CSP B, CSPB, CTLA1, CTSGL1, Endogenous granzyme B, Granzyme B, GZMB, HLP, SECT



Tested Applications

Positive WB detected inHL-60 cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

13588-1-AP targets Granzyme B in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Granzyme B fusion protein Ag3883
Full Name granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)
Calculated molecular weight 247 aa, 28 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC030195
Gene symbol GZMB
Gene ID (NCBI) 3002
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GZMB(Granzyme B) is also named as CGL1, CSPB, CTLA1, GRB and belongs to the Granzyme subfamily. This enzyme is necessary for target cell lysis in cell-mediated immune responses. The cytotoxic lymphocyte protease granzyme B (GzmB) can promote apoptosis through direct processing and activation of members of the caspase family. GzmB can also cleave the BH3-only protein, BID, to promote caspase-independent mitochondrial permeabilization (PMID:17283187). GzmB induces laminB degradation in isolated nuclei less efficiently than GzmA (PMID:11331782). This full length protein has 2 glycosylation sites and a signal peptide. Unglycosylated human granzyme B is 26 kDa and g high mannose glycosylated is 32 kDa and only 32kDa or smaller forms of granzyme B are accumulated within nuclei (PMID:8626751).

Protocols

Product Specific Protocols
WB protocol for Granzyme B antibody 13588-1-APDownload protocol
IHC protocol for Granzyme B antibody 13588-1-APDownload protocol
IF protocol for Granzyme B antibody 13588-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

J Am Acad Dermatol

Generalized bullous fixed drug eruption is distinct from Stevens-Johnson syndrome/toxic epidermal necrolysis by immunohistopathological features.

Authors - Yung-Tsu Cho
humanWB

Mol Immunol

VEGF165b augments NK92 cytolytic activity against human K562 leukemia cells by upregulating the levels of perforin and granzyme B via the VEGR1-PLC pathway.

Authors - Xiaoyin Wang
humanIHC

Int Immunopharmacol

Apatinib enhanced anti-PD-1 therapy for colon cancer in mice via promoting PD-L1 expression.

Authors - Xiaomin Cai
humanIHC

Front Oncol

Transcriptome-Based Network Analysis Unveils Eight Immune-Related Genes as Molecular Signatures in the Immunomodulatory Subtype of Triple-Negative Breast Cancer.

Authors - Jinguo Zhang
humanWB,IF

Cell Mol Life Sci

Granzyme B is recovered by natural killer cells via clathrin-dependent endocytosis.

Authors - Li Pan P
humanIHC

J Hematol Oncol

PD-1 axis expression in musculoskeletal tumors and antitumor effect of nivolumab in osteosarcoma model of humanized mouse.

Authors - Bingxin Zheng