Granzyme K Polyclonal antibody

Granzyme K Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 27894-1-AP

Synonyms

Fragmentin 3, Granzyme 3, Granzyme K, GZMK, NK Tryp 2, NK tryptase 2, TRYP2



Tested Applications

Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

27894-1-AP targets Granzyme K in IHC, IF, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Granzyme K fusion protein Ag27281
Full Name granzyme K (granzyme 3; NK tryptase II)
Calculated molecular weight 29 kDa
GenBank accession numberBC035802
Gene symbol GZMK
Gene ID (NCBI) 3003
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Granzymes are a family of serine proteases stored in granules inside cytotoxic cells of the immune system. There are five human granzymes (granzyme A (GrA), GrB, GrH, GrK and GrM) currently identified, whereas mice have ten known granzymes (GrA-G, GrK, GrM and GrN) (PMID:14499263). Human GrK was first discovered in 1988 after purification from human peripheral blood mononuclear cells. GrK is expressed by cytotoxic T lymphocytes, natural killer T cells (NKT), γδ T cells and CD56bright+ NK cells.

Protocols

Product Specific Protocols
IHC protocol for Granzyme K antibody 27894-1-APDownload protocol
IF protocol for Granzyme K antibody 27894-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols