Granzyme K Polyclonal antibody

Granzyme K Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 27894-1-AP

Synonyms

Fragmentin 3, Granzyme 3, Granzyme K, GZMK, NK Tryp 2, NK tryptase 2, TRYP2

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Validation Data Gallery

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  IHC staining of human tonsillitis using 27894-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27894-1-AP (GZMK antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 27894-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27894-1-AP (GZMK antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 27894-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27894-1-AP (GZMK antibody) at dilution of 1:500 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of NIH/3T3 using 27894-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using 27894-1-AP (GZMK antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: NIH/3T3 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Product Information

27894-1-AP targets Granzyme K in IHC, IF, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Granzyme K fusion protein Ag27281
• Full Name: granzyme K (granzyme 3; NK tryptase II)
• Calculated molecular weight: 29 kDa
• GenBank accession number: BC035802
• Gene symbol: GZMK
• Gene ID (NCBI): 3003
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Granzymes are a family of serine proteases stored in granules inside cytotoxic cells of the immune system. There are five human granzymes (granzyme A (GrA), GrB, GrH, GrK and GrM) currently identified, whereas mice have ten known granzymes (GrA-G, GrK, GrM and GrN) (PMID:14499263). Human GrK was first discovered in 1988 after purification from human peripheral blood mononuclear cells. GrK is expressed by cytotoxic T lymphocytes, natural killer T cells (NKT), γδ T cells and CD56bright+ NK cells.