Granzyme K Polyclonal antibody
Granzyme K Polyclonal Antibody for IF, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, mouse
Applications
IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 27894-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | NIH/3T3 cells |
Recommended dilution
Application | Dilution |
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Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Product Information
27894-1-AP targets Granzyme K in IHC, IF, ELISA applications and shows reactivity with Human, mouse samples.
Tested Reactivity | Human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Granzyme K fusion protein Ag27281 |
Full Name | granzyme K (granzyme 3; NK tryptase II) |
Calculated molecular weight | 29 kDa |
GenBank accession number | BC035802 |
Gene symbol | GZMK |
Gene ID (NCBI) | 3003 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Granzymes are a family of serine proteases stored in granules inside cytotoxic cells of the immune system. There are five human granzymes (granzyme A (GrA), GrB, GrH, GrK and GrM) currently identified, whereas mice have ten known granzymes (GrA-G, GrK, GrM and GrN) (PMID:14499263). Human GrK was first discovered in 1988 after purification from human peripheral blood mononuclear cells. GrK is expressed by cytotoxic T lymphocytes, natural killer T cells (NKT), γδ T cells and CD56bright+ NK cells.
Protocols
Product Specific Protocols | |
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IHC protocol for Granzyme K antibody 27894-1-AP | Download protocol |
IF protocol for Granzyme K antibody 27894-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |