Glypican 3 Polyclonal antibody

Glypican 3 Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG


human and More (1)





Cat no : 25175-1-AP


DGSX, Glypican 3, GPC3, GTR2 2, Intestinal protein OCI 5, MXR7, OCI 5, OCI5, SDYS, SGB, SGBS, SGBS1

Tested Applications

Positive WB detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:1000
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 25175-1-AP is Glypican 3 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Glypican 3 fusion protein Ag10129
Full Name glypican 3
Calculated molecular weight 580 aa, 66 kDa
Observed molecular weight 66 kDa
GenBank accession numberBC035972
Gene symbol GPC3
Gene ID (NCBI) 2719
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Glypicans (GPCs) are a family of glycosylphosphatidylinositol (GPI)-anchored heparan sulphate proteoglycans (HSPGs) that may play a role in the control of cell division and growth regulation. In mammals, there are six GPCs (GPC1 to GPC6), all of which have a similar core-protein size of approx. 60 kDa and the clustering of glycosaminoglycan attachment site near the C-terminus. They are tethered to the cell surface by GPI linkages, which can be cleaved by endogenous phospholipases, thus releasing the protein. Glypican 3 (GPC3) is highly expressed in many tissues during development and plays an important role in the regulation of embryonic growth (PMID: 22467855). Loss-of-function mutations of GPC3 result in the Simpson-Golabi-Behmel overgrowth syndrome (SGBS), and Gpc-3 null mice display developmental overgrowth (PMID: 8589713; 18477453). In hepatocellular carcinoma (HCC), the overexpression of glypican 3 has been demonstrated to be a reliable diagnostic indicator (PMID: 19212669; 22706665). The calculated molecular weight of native glypican 3 is 66 kDa, glycanated forms of glypican 3 have higher molecular weights than 66 kDa (PMID: 12851874; 16024626; 19574424).


Product Specific Protocols
WB protocol for Glypican 3 antibody 25175-1-APDownload protocol
Standard Protocols
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