Glypican 3 Polyclonal antibody, PBS Only

• 

  WB analysis of HepG2 using 25175-1-AP (same clone as 25175-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 25175-1-AP (Glypican 3 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25175-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 25175-1-AP (same clone as 25175-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 25175-1-AP (Glypican 3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25175-1-PBS in a different storage buffer formulation.

Glypican 3 Polyclonal Antibody for WB, Indirect ELISA

Cat No. 25175-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

GPC3, DGSX, Glypican-3 alpha subunit, Glypican-3 beta subunit, GTR2 2

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Product Information

25175-1-PBS targets Glypican 3 in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag10129

  Product name: Recombinant human GPC3 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 232-580 aa of BC035972

  Sequence: ALNLGIEVINTTDHLKFSKDCGRMLTRMWYCSYCQGLMMVKPCGGYCNVVMQGCMAGVVEIDKYWREYILSLEELVNGMYRIYDMENVLLGLFSTIHDSIQYVQKNAGKLTTTIGKLCAHSQQRQYRFAYYPEDLFIDKKVLKVAHVEHEETLSSRRRELIQKLKSFISFYSALPGYICSHSPVAENDTLCWNGQELVERYSQKAARNGMKNQFNLHELKMKGPEPVVSQIIDKLKHINQLLRTMSMPKGRVLDKNLDEEGFESGDCGDDEDECIGGSGDGMIKVKNQLRFLAELAYDLDVDDAPGNSQQATPKDNEISTFHNLGNVHSPLKLLTSMAISVVCFFFLVH

• Full Name: glypican 3
• Calculated Molecular Weight: 580 aa, 66 kDa
• Observed Molecular Weight: 66 kDa
• GenBank Accession Number: BC035972
• Gene Symbol: GPC3
• Gene ID (NCBI): 2719
• RRID: AB_2879942
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P51654
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Glypicans (GPCs) are a family of glycosylphosphatidylinositol (GPI)-anchored heparan sulphate proteoglycans (HSPGs) that may play a role in the control of cell division and growth regulation. In mammals, there are six GPCs (GPC1 to GPC6), all of which have a similar core-protein size of approx. 60 kDa and the clustering of glycosaminoglycan attachment site near the C-terminus. They are tethered to the cell surface by GPI linkages, which can be cleaved by endogenous phospholipases, thus releasing the protein. Glypican 3 (GPC3) is highly expressed in many tissues during development and plays an important role in the regulation of embryonic growth (PMID: 22467855). Loss-of-function mutations of GPC3 result in the Simpson-Golabi-Behmel overgrowth syndrome (SGBS), and Gpc-3 null mice display developmental overgrowth (PMID: 8589713; 18477453). In hepatocellular carcinoma (HCC), the overexpression of glypican 3 has been demonstrated to be a reliable diagnostic indicator (PMID: 19212669; 22706665). The calculated molecular weight of native glypican 3 is 66 kDa, glycanated forms of glypican 3 have higher molecular weights than 66 kDa (PMID: 12851874; 16024626; 19574424).