HADH Polyclonal antibody

HADH Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

23

Cat no : 19828-1-AP

Synonyms

HAD, HADH, HADH1, HADHSC, HCDH, HHF4, M/SCHAD, SCHAD

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Validation Data Gallery

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  WB analysis using 19828-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19828-1-AP (HADH antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 19828-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 19828-1-AP (HADH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 19828-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 19828-1-AP (HADH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 19828-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 19828-1-AP (HADH antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 19828-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 19828-1-AP (HADH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 19828-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19828-1-AP (HADH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 19828-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 19828-1-AP (HADH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 19828-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 19828-1-AP (HADH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 19828-1-AP

  IP result of anti-HADH (IP:19828-1-AP, 3ug; Detection:19828-1-AP 1:700) with HepG2 cells lysate 400ug.

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  IHC staining of human breast cancer using 19828-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19828-1-AP (HADH antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 19828-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19828-1-AP (HADH antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 19828-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19828-1-AP (HADH antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hepatocirrhosis using 19828-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 19828-1-AP (HADH antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human hepatocirrhosis using 19828-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 19828-1-AP (HADH antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human hepatocirrhosis using 19828-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 19828-1-AP (HADH antibody) at dilution of 1:400 (under 10x lens).

• 

  IHC staining of human hepatocirrhosis using 19828-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 19828-1-AP (HADH antibody) at dilution of 1:400 (under 40x lens).

• 

  IF Staining of HepG2 using 19828-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 19828-1-AP (HADH antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, Jurkat cells, mouse heart tissue, mouse kidney tissue, mouse liver tissue, mouse pancreas tissue, mouse skeletal muscle tissue, rat heart tissue
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human breast cancer tissue, human liver cancer tissue, human hepatocirrhosis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 22 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 2 publications below

Product Information

19828-1-AP targets HADH in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HADH fusion protein Ag13928
• Full Name: hydroxyacyl-Coenzyme A dehydrogenase
• Calculated Molecular Weight: 314 aa, 34 kDa
• Observed Molecular Weight: 30-34 kDa
• GenBank Accession Number: BC000306
• Gene Symbol: HADH
• Gene ID (NCBI): 3033
• RRID: AB_10667408
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HADH(Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial) is also named as HAD, HADHSC, SCHAD and belongs to the 3-hydroxyacyl-CoA dehydrogenase family. It catalyzes the reversible dehydrogenation of 3-hydroxyacyl-CoAs to their corresponding 3-ketoacyl-CoAs with concomitant reduction of NAD to NADH and exerts it highest activity toward 3-hydroxydecanoyl-CoA. . Human HADH encodes a deduced 314-amino acid protein composed of a 12-residue mitochondrial import signal peptide and a 302-residue mature HADH protein with a calculated molecular mass of 34.3 kD.

Publications

Species	Application	Title
mouse	WB	Cell Metab; Pyruvate-supported flux through medium-chain ketothiolase promotes mitochondrial lipid tolerance in cardiac and skeletal muscles; Authors - Timothy R Koves
human	IP, WB	Hepatology; Disruption of the histidine triad nucleotide-binding hint2 gene in mice affects glycemic control and mitochondrial function.; Authors - Martin Juliette J
mouse	WB	EMBO Rep; SCFJFK is functionally linked to obesity and metabolic syndrome.; Authors - Lin He
rat	WB	EBioMedicine; PPARγ maintains the metabolic heterogeneity and homeostasis of renal tubules.; Authors - Zhongshi Lyu
human	WB	Front Oncol; Multi-Omics Analysis of Fatty Acid Metabolism in Thyroid Carcinoma.; Authors - Jinghui Lu
mouse	WB	Sci Rep; Targeted Metabolomics Reveals Abnormal Hepatic Energy Metabolism by Depletion of β-Carotene Oxygenase 2 in Mice.; Authors - Lei Wu