HADH Polyclonal antibody

HADH Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 19828-1-AP

Synonyms

HAD, HADH, HADH1, HADHSC, HCDH, HHF4, M/SCHAD, SCHAD



Tested Applications

Positive WB detected inHepG2 cells, Jurkat cells, mouse heart tissue, mouse kidney tissue, mouse liver tissue, mouse pancreas tissue, mouse skeletal muscle tissue, rat heart tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue, human hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 19828-1-AP is HADH Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HADH fusion protein Ag13928
Full Name hydroxyacyl-Coenzyme A dehydrogenase
Calculated molecular weight 314 aa, 34 kDa
Observed molecular weight 30-34 kDa
GenBank accession numberBC000306
Gene symbol HADH
Gene ID (NCBI) 3033
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HADH(Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial) is also named as HAD, HADHSC, SCHAD and belongs to the 3-hydroxyacyl-CoA dehydrogenase family. It catalyzes the reversible dehydrogenation of 3-hydroxyacyl-CoAs to their corresponding 3-ketoacyl-CoAs with concomitant reduction of NAD to NADH and exerts it highest activity toward 3-hydroxydecanoyl-CoA. . Human HADH encodes a deduced 314-amino acid protein composed of a 12-residue mitochondrial import signal peptide and a 302-residue mature HADH protein with a calculated molecular mass of 34.3 kD.

Protocols

Product Specific Protocols
WB protocol for HADH antibody 19828-1-APDownload protocol
IHC protocol for HADH antibody 19828-1-APDownload protocol
IF protocol for HADH antibody 19828-1-APDownload protocol
IP protocol for HADH antibody 19828-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Biophotonics

PMID: 31707768

Authors - Gabriela Silva
WB

Front Physiol

PMID: 30429793

Authors - Mariana Aguiar de Matos
humanWB

Mol Nutr Food Res

Lack of β, β-carotene -9', 10'-oxygenase 2 leads to hepatic mitochondrial dysfunction and cellular oxidative stress in mice.

Authors - Lei Wu
ratWB

Nutrients

Effects of a Ketogenic Diet Containing Medium-Chain Triglycerides and Endurance Training on Metabolic Enzyme Adaptations in Rat Skeletal Muscle.

Authors - Ayumi Fukazawa
mouseWB

J Physiol

Piezo1 channels mediate trabecular meshwork mechanotransduction and promote aqueous fluid outflow.

Authors - Oleg Yarishkin
mouseWB

Sci Rep

Targeted Metabolomics Reveals Abnormal Hepatic Energy Metabolism by Depletion of β-Carotene Oxygenase 2 in Mice.

Authors - Lei Wu