HADH Polyclonal antibody

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• HADH Polyclonal antibody (19828-1-AP)

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HADH Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 19828-1-AP Copied!

Publications (37)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

HCDH, HADHSC, HADH1, HAD1, HAD

• 

  WB analysis using 19828-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19828-1-AP (HADH antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of A2780 using 19828-1-AP

  WB result of HADH antibody (19828-1-AP; 1:6000; room temperature for 1.5 hours) with wild-type and HADH knockout A2780 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HepG2 using 19828-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 19828-1-AP (HADH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse pancreas using 19828-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 19828-1-AP (HADH antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse skeletal muscle using 19828-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 19828-1-AP (HADH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of Jurkat using 19828-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19828-1-AP (HADH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse heart using 19828-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 19828-1-AP (HADH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse kidney using 19828-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 19828-1-AP (HADH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse liver using 19828-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 19828-1-AP (HADH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HepG2 using 19828-1-AP

  IP result of anti-HADH (IP:19828-1-AP, 3ug; Detection:19828-1-AP 1:700) with HepG2 cells lysate 400ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 19828-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19828-1-AP (HADH antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 19828-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19828-1-AP (HADH antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 19828-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19828-1-AP (HADH antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human hepatocirrhosis using 19828-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 19828-1-AP (HADH antibody) at dilution of 1:200 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human hepatocirrhosis using 19828-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 19828-1-AP (HADH antibody) at dilution of 1:200 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human hepatocirrhosis using 19828-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 19828-1-AP (HADH antibody) at dilution of 1:400 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human hepatocirrhosis using 19828-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 19828-1-AP (HADH antibody) at dilution of 1:400 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 19828-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 19828-1-AP (HADH antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HepG2 cells, A2780 cells, Jurkat cells, mouse heart tissue, mouse kidney tissue, mouse liver tissue, mouse pancreas tissue, mouse skeletal muscle tissue, rat heart tissue
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human breast cancer tissue, human liver cancer tissue, human hepatocirrhosis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19828-1-AP targets HADH in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag13928

  Product name: Recombinant human HADH protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-314 aa of BC000306

  Sequence: MAFVTRQFMRSVSSSSTASASAKKIIVKHVTVIGGGLMGAGIAQVAAATGHTVVLVDQTEDILAKSKKGIEESLRKVAKKKFAENPKAGDEFVEKTLSTIATSTDAASVVHSTDLVVEAIVENLKVKNELFKRLDKFAAEHTIFASNTSSLQITSIANATTRQDRFAGLHFFNPVPVMKLVEVIKTPMTSQKTFESLVDFSKALGKHPVSCKDTPGFIVNRLLVPYLMEAIRLYERGDASKEDIDTAMKLGAGYPMGPFELLDYVGLDTTKFIVDGWHEMDAENPLHQPSPSLNKLVAENKFGKKTGEGFYKYK

• Full Name: hydroxyacyl-Coenzyme A dehydrogenase
• Calculated Molecular Weight: 314 aa, 34 kDa
• Observed Molecular Weight: 30-34 kDa
• GenBank Accession Number: BC000306
• Gene Symbol: HADH
• Gene ID (NCBI): 3033
• RRID: AB_10667408
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q16836
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HADH(Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial) is also named as HAD, HADHSC, SCHAD and belongs to the 3-hydroxyacyl-CoA dehydrogenase family. It catalyzes the reversible dehydrogenation of 3-hydroxyacyl-CoAs to their corresponding 3-ketoacyl-CoAs with concomitant reduction of NAD to NADH and exerts it highest activity toward 3-hydroxydecanoyl-CoA. . Human HADH encodes a deduced 314-amino acid protein composed of a 12-residue mitochondrial import signal peptide and a 302-residue mature HADH protein with a calculated molecular mass of 34.3 kD.

Publications

What published studies show

The HADH Polyclonal antibody (Cat# 19828-1-AP) has 37 citations across journals including Cell Metabolism, Cancer Research, Hepatology, Cell Discovery, Redox Biology, EBioMedicine, Free Radical Biology and Medicine, EMBO Molecular Medicine, iScience, GeroScience, Mitochondrion, Scientific Reports, Frontiers in Immunology, and others. Research fields span mitochondrial fatty acid oxidation, cardiac and skeletal muscle metabolism, cancer (HCC, colorectal, cervical, thyroid), obesity, insulin resistance, ischemia-reperfusion injury, aging, and lipid homeostasis.

Automatically generated from the 37 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Cell Metab; Pyruvate-supported flux through medium-chain ketothiolase promotes mitochondrial lipid tolerance in cardiac and skeletal muscles; Authors - Timothy R Koves
		Cancer Res; Long-Chain Fatty Acid Redistribution Induced by SLC27A2 Deficiency Facilitates Hypoxic Adaptation and Immunosuppression in Hepatocellular Carcinoma; Authors - Chuli Fu
human	IP, WB	Hepatology; Disruption of the histidine triad nucleotide-binding hint2 gene in mice affects glycemic control and mitochondrial function.; Authors - Martin Juliette J
human	IHC	Cell Discov; Integrated proteogenomic characterization of ampullary adenocarcinoma; Authors - Qiao Zhang
		Redox Biol; Inhibition of eIF5A hypusination enhances antioxidant defense to prevent kidney Ischemia/Reperfusion injury; Authors - Marc Cougnon
rat	WB	EBioMedicine; PPARγ maintains the metabolic heterogeneity and homeostasis of renal tubules.; Authors - Zhongshi Lyu