HAS2 Recombinant antibody, PBS Only (Capture)

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  WB analysis of HEK-293 using 83204-2-RR (same clone as 83204-2-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 83204-2-RR (HAS2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83204-2-PBS in a different storage buffer formulation.

• 

  WB analysis using 83204-2-RR (same clone as 83204-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83204-2-RR (HAS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83204-2-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 83204-2-RR (same clone as 83204-2-PBS)

  WB result of HAS2 antibody (83204-2-RR; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-HAS2 transfected HEK-293 cells. This data was developed using the same antibody clone with 83204-2-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP00153-1

  Cytometric bead array standard curve of MP00153-1, HAS2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83204-2-PBS. Detection antibody: 83204-1-PBS. Standard: Ag34945. Range: 0.625-20 ng/mL.

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  Cytometric bead array standard curve of MP00153-3

  Cytometric bead array standard curve of MP00153-3, HAS2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83204-2-PBS. Detection antibody: 83204-3-PBS. Standard: Ag34945. Range: 0.625-20 ng/mL.

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  Affinity and Kinetic Characterization of 83204-2-PBS

  Biolayer interferometry (BLl) kinetic assay of 83204-2-PBS against Human HAS2 was performed. The affinity constant is 0.516 nM.

• 

  Affinity and Kinetic Characterization of 83204-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83204-2-RR against Human HAS2 were performed. The affinity constant is 1.37 nM.

• Featured Product
• KD/KO Validated

HAS2 Uni-rAb^TM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Cat No. 83204-2-PBS

Clone No.240061G4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Cytometric bead array, Indirect ELISA

Synonyms

240061G4, EC:2.4.1.212, HA synthase 2, hyaluronan synthase 2, Hyaluronate synthase 2

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Product Information

83204-2-PBS targets HAS2 as part of a matched antibody pair:

MP00153-1: 83204-2-PBS capture and 83204-1-PBS detection (validated in Cytometric bead array)

MP00153-3: 83204-2-PBS capture and 83204-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: HAS2 fusion protein Ag34945
• Full Name: hyaluronan synthase 2
• Calculated Molecular Weight: 552 aa, 64 kDa
• Observed Molecular Weight: 63-67 kDa
• GenBank Accession Number: BC069353
• Gene Symbol: HAS2
• Gene ID (NCBI): 3037
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q92819
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

RIT2 (Hyaluronan synthase 2) is a member of the gene family encoding the hyaluronan synthase 2, which can generate high-molecular-weight hyaluronan (HMW-HA) (PMID: 35069543). HAS2 is the most critical synthase in producing hyaluronan and is well known for its involvement in cancer growth, metabolism and metastasis (PMID: 36386154). HAS2 is involved in cellular acquired resistance to drug therapy in BrCa. HAS2 expression was decreased in the endocrine-resistant ER+BrCa cells.