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  • KD/KO Validated

HAX1 Polyclonal antibody

HAX1 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 11266-1-AP

Synonyms

HAX 1, HAX1, HCLS1 associated protein X 1, HCLSBP1, HS1 associating protein X 1, HS1 binding protein 1, HS1BP1, HSP1BP 1, SCN3



Tested Applications

Positive WB detected inHeLa cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11266-1-AP targets HAX1 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity hamster, human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HAX1 fusion protein Ag1792
Full Name HCLS1 associated protein X-1
Calculated molecular weight 32 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC014314
Gene symbol HAX1
Gene ID (NCBI) 10456
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HAX1, also named as HS1BP1, is a 32 kDa protein that is ubiquitously expressed. Hax1 has been demonstrated to be a negative regulator of apoptosis in many immune cell types. Furthermore, Hax1 has been shown to have additional roles in regulating cell motility and adhesion, and is overexpressed in many types of cancer. Kristina and Cavnar demonstrate in their paper validation of two research antibodies including Proteintech's anti-Hax1 (11266-1-AP). They have evidence that the rabbit anti-Hax1 (11266-1-AP, Proteintech Group Inc.) results in a more robust and consistent detection of Hax1.

Protocols

Product Specific Protocols
WB protocol for HAX1 antibody 11266-1-APDownload protocol
IHC protocol for HAX1 antibody 11266-1-APDownload protocol
IP protocol for HAX1 antibody 11266-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

F1000Res

Immunoblotting validation of research antibodies generated against HS1-associated protein X-1 in the human neutrophil model cell line PLB-985.

Authors - Peter Cavnar
humanWB,IF

Thorac Cancer

HAX1 enhances the survival and metastasis of non-small cell lung cancer through the AKT/mTOR and MDM2/p53 signaling pathway.

Authors - Zhigang Liang
  • KD Validated
humanWB

Fish Shellfish Immunol

Lamprey PHB2 maintains mitochondrial stability by tanslocation to the mitochondria under oxidative stress.

Authors - Ying Shi
humanWB

J Cell Biol

Hax1 regulates neutrophil adhesion and motility through RhoA.

Authors - Cavnar Peter J PJ
  • KD Validated
hamsterWB,IP

Nat Commun

Cerebellar Kv3.3 potassium channels activate TANK-binding kinase 1 to regulate trafficking of the cell survival protein Hax-1.

Authors - Yalan Zhang
humanWB

Mol Cell

The IMiD target CRBN determines HSP90 activity toward transmembrane proteins essential in multiple myeloma.

Authors - Michael Heider