|Positive WB detected in||HeLa cells|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human lymphoma tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
11266-1-AP targets HAX1 in WB, IP, IHC, IF applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, hamster|
|Host / Isotype||Rabbit / IgG|
|Immunogen||HAX1 fusion protein Ag1792|
|Full Name||HCLS1 associated protein X-1|
|Calculated molecular weight||32 kDa|
|Observed molecular weight||32 kDa|
|GenBank accession number||BC014314|
|Gene ID (NCBI)||10456|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
HAX1, also named as HS1BP1, is a 32 kDa protein that is ubiquitously expressed. Hax1 has been demonstrated to be a negative regulator of apoptosis in many immune cell types. Furthermore, Hax1 has been shown to have additional roles in regulating cell motility and adhesion, and is overexpressed in many types of cancer. Kristina and Cavnar demonstrate in their paper validation of two research antibodies including Proteintech's anti-Hax1 (11266-1-AP). They have evidence that the rabbit anti-Hax1 (11266-1-AP, Proteintech Group Inc.) results in a more robust and consistent detection of Hax1.
Immunoblotting validation of research antibodies generated against HS1-associated protein X-1 in the human neutrophil model cell line PLB-985.
HAX1 enhances the survival and metastasis of non-small cell lung cancer through the AKT/mTOR and MDM2/p53 signaling pathway.
Fish Shellfish Immunol
Lamprey PHB2 maintains mitochondrial stability by tanslocation to the mitochondria under oxidative stress.
J Cell Mol Med
HAX1 maintains the glioma progression in hypoxia through promoting mitochondrial fission.
Suppression of Kv3.3 channels by antisense oligonucleotides reverses biochemical effects and motor impairment in spinocerebellar ataxia type 13 mice.
J Cell Biol
Hax1 regulates neutrophil adhesion and motility through RhoA.