HDAC1 Recombinant antibody, PBS Only (Capture)

• 

  WB analysis using 83624-1-RR (same clone as 83624-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83624-1-RR (HDAC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83624-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse colon using 83624-1-RR (same clone as 83624-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 83624-1-RR (HDAC1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83624-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 83624-1-RR (same clone as 83624-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HDAC1 antibody (83624-1-RR, Clone: 240614A3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83624-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 83624-1-RR (same clone as 83624-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HDAC1 antibody (83624-1-RR, Clone: 240614A3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83624-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 83624-1-RR (same clone as 83624-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug HDAC1 Recombinant antibody (83624-1-RR, Clone:240614A3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83624-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP00577-3

  Cytometric bead array standard curve of MP00577-3, HDAC1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83624-1-PBS. Detection antibody: 83624-3-PBS. Standard: Ag0256. Range: 0.625-80 ng/mL.

• 

  Affinity and Kinetic Characterization of 83624-1-PBS

  Biolayer interferometry (BLl) kinetic assay of 83624-1-PBS against Human HDAC1 was performed. The affinity constant is below 1 pM.

• 

  Affinity and Kinetic Characterization of 83624-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83624-1-RR against Human HDAC1 were performed. The affinity constant is below 1 pM.

HDAC1 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Cat No. 83624-1-PBS

Clone No.240614A3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Synonyms

histone deacetylase 1, HD1, GON 10, EC:3.5.1.98, EC:3.5.1.-

---

Product Information

83624-1-PBS targets HDAC1 as part of a matched antibody pair:

MP00577-3: 83624-1-PBS capture and 83624-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: HDAC1 fusion protein Ag0256
• Full Name: histone deacetylase 1
• Calculated Molecular Weight: 55 kDa
• Observed Molecular Weight: 58-66 kDa
• GenBank Accession Number: BC000301
• Gene Symbol: HDAC1
• Gene ID (NCBI): 3065
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q13547
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex, which is responsible for gene expression silencing. It also plays an important role in the control of cell proliferation and differentiation by interacting with RB, p53 and other transcription factors. At least 4 classes of HDAC were identified. As a class I HDAC, HDAC 1 was primarily found in the nucleus.