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CoraLite® Plus 488-conjugated HDAC1 Polyclonal antibody
HDAC1 Polyclonal Antibody for FC (Intra)
Host / Isotype
Rabbit / IgG
human, mouse, rat
CoraLite® Plus 488 Fluorescent Dye
Cat no : CL488-10197
|Positive FC detected in||HeLa cells|
|Sample-dependent, check data in validation data gallery|
CL488-10197 targets HDAC1 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||HDAC1 fusion protein Ag0256|
|Full Name||histone deacetylase 1|
|Calculated molecular weight||55 kDa|
|Observed molecular weight||60 kDa|
|GenBank accession number||BC000301|
|Gene ID (NCBI)||3065|
|Conjugate||CoraLite® Plus 488 Fluorescent Dye|
|Excitation/Emission maxima wavelengths||493 nm / 522 nm|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.|
|Storage Conditions||Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
HDAC1 poly https://www.ptglab.com/products/HDAC1-Antibody-10197-1-AP.htm
Histone Deacetylase 1 (HDAC1) is a nuclear localized class I histone deacetylase that plays a role in the regulation of gene expression, mainly by repression of gene activity. Additionally, HDAC can mediate deacetylation of a subset of non-histone proteins, leading to their degradation. HDAC1 activity has an impact on cell growth, proliferation, and death.
1. What is the molecular weight of HDAC1?
The molecular size of HDAC1 is 60 kDa.
2. What is the subcellular localization of HDAC1?
Unlike some HDACs, HDAC1 is present entirely in the nucleus. Our HDAC1 antibody has been broadly tested for IF/ICC.
3. I cannot detect an HDAC1 specific signal in my sample during western blotting.
Make sure that you efficiently extract nuclear proteins during your sample preparation. Some lysis buffers (e.g., based on Triton X-100) may not extract nuclear proteins. We recommend using RIPA buffer (https://www.ptglab.com/support/protocols/). We highly recommend using nuclear loading control antibodies, such as lamin B1 or PCNA (https://www.ptglab.com/news/blog/loading-control-antibodies-for-western-blotting/). Alternatively, you may consider performing cell fractionation.
4. How do I perform chromatin immunoprecipitation (ChiP) with the HDAC1 antibody?
We recommend using our standard chromatin immunoprecipitation protocol (https://www.ptglab.com/media/2708/web_chip-protocol.pdf).
5. Is HDAC1 post-translationally modified?
HDAC1 is a protein deacetylase but itself can also be a subject of post-translational modifications, including phosphorylation, acetylation, ubiquitination, SUMOylation, nitrosylation, and carbonylation (PMID: 21197454).
6. What is the role of HDAC1 in cancer?
The acetylation and deacetylation of histones play an important part in the epigenetic regulation of gene expression. Alterations in the balance between these two opposing processes changes chromosome remodeling and increased deacetylation leads to gene silencing. HDAC1 levels are increased in many cancer cell types and the role of HDAC1 in cancer is connected not only to histone deacetylation but also to the deacetylation of other proteins, such as Rb family proteins, estrogen receptors, and p53 protein (PMID: 19383284).
|Product Specific Protocols|
|FC protocol for CL Plus 488 HDAC1 antibody CL488-10197||Download protocol|
|Click here to view our Standard Protocols|