HDAC5 Polyclonal antibody, PBS Only

• 

  WB analysis using 29342-1-AP (same clone as 29342-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29342-1-AP (HDAC5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29342-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 29342-1-AP (same clone as 29342-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29342-1-AP (HDAC5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29342-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 29342-1-AP (same clone as 29342-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HDAC5 antibody (29342-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 29342-1-PBS in a different storage buffer formulation.

HDAC5 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 29342-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

---

Product Information

29342-1-PBS targets HDAC5 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HDAC5 fusion protein Ag29122
• Full Name: histone deacetylase 5
• Calculated Molecular Weight: 122 kDa
• Observed Molecular Weight: 120-140 kDa
• GenBank Accession Number: NM_005474
• Gene Symbol: HDAC5
• Gene ID (NCBI): 10014
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UQL6
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Histone acetylation and deacetylation alternately exposes and occludes DNA to transcription factors. At least 4 classes of HDAC were identified. HDAC5 is a class II HDAC. HDAC5 responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. HDAC5 is involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, HDAC5 shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors.