HERC5 Polyclonal antibody

HERC5 Polyclonal Antibody for IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 22692-1-AP

Synonyms

CEB1, CEBP1, Cyclin E binding protein 1, E3 ISG15 protein ligase HERC5, hect domain and RLD 5, HERC5

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Validation Data Gallery

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  IP experiment of HEK-293 using 22692-1-AP

  IP result of anti-HERC5 (IP:22692-1-AP, 4ug; Detection:22692-1-AP 1:300) with HEK-293 cells lysate 2800ug.

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  IHC staining of mouse testis using 22692-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 22692-1-AP (HERC5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 22692-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 22692-1-AP (HERC5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IP detected in: HEK-293 cells

Recommended dilution

• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• CoIP: See 1 publications below

Product Information

The immunogen of 22692-1-AP is HERC5 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HERC5 fusion protein Ag18449
• Full Name: hect domain and RLD 5
• Calculated Molecular Weight: 1024 aa, 117 kDa
• Observed Molecular Weight: 117 kDa
• GenBank Accession Number: BC140716
• Gene Symbol: HERC5
• Gene ID (NCBI): 51191
• RRID: AB_2879151
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	MBio; ISG15 Deficiency Enhances HIV-1 Infection by Accumulating Misfolded p53.; Authors - Edmund Osei Kuffour
human	WB	Int J Mol Med; ISG15 inhibits cancer cell growth and promotes apoptosis.; Authors - Mei-Juan Zhou
human,mouse	WB,CoIP	J Clin Invest; Posttranslational ISGylation of NLRP3 by HERCs enzymes facilitates inflammasome activation in models of inflammation; Authors - Ying Qin ; KD Validated
human	WB	Cell Rep; Regulation of STING activity in DNA sensing by ISG15 modification; Authors - Chaohui Lin