HERC5 Polyclonal antibody

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  IP experiment of HEK-293 using 22692-1-AP

  IP result of anti-HERC5 (IP:22692-1-AP, 4ug; Detection:22692-1-AP 1:500) with HEK-293 cells lysate 1400 ug.

HERC5 Polyclonal Antibody for IP, ELISA

Cat No. 22692-1-AP

Publications(10)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IP, CoIP, ELISA

Synonyms

CEB1, CEBP1, Cyclin E binding protein 1, Cyclin-E-binding protein 1, E3 ISG15 protein ligase HERC5

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Tested Applications

• Positive IP detected in: HEK-293 cells

Recommended dilution

• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• IHC: See 2 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below

Product Information

22692-1-AP targets HERC5 in IHC, IP, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag18449

  Product name: Recombinant human HERC5 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-342 aa of BC140716

  Sequence: MERRSRRKSRRNGRSTAGKAAATQPAKSPGAQLWLFPSAAGLHRALLRRVEVTRQLCCSPGRLAVLERGGAGVQVHQLLAGSGGARTPKCIKLGKNMKIHSVDQGAEHMLILSSDGKPFEYDNYSMKHLRFESILQEKKIIQITCGDYHSLALSKGGELFAWGQNLHGQLGVGRKFPSTTTPQIVEHLAGVPLAQISAGEAHSMALSMSGNIYSWGKNECGQLGLGHTESKDDPSLIEGLDNQKVEFVACGGSHSALLTQDGLLFTFGAGKHGQLGHNSTQNELRPCLVAELVGYRVTQIACGRWHTLAYVSDLGKVFSFGSGKDGQLGNGGTRDQLMPLPV

• Full Name: hect domain and RLD 5
• Calculated Molecular Weight: 1024 aa, 117 kDa
• Observed Molecular Weight: 117 kDa
• GenBank Accession Number: BC140716
• Gene Symbol: HERC5
• Gene ID (NCBI): 51191
• RRID: AB_2879151
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• UNIPROT ID: Q9UII4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HERC5 (HECT and RLD domain-containing E3 ubiquitin ligase 5) is constitutively expressed at low or undetectable levels in most normal adult tissues and in a subset of cancer cell lines. It is an evolutionarily ancient restriction factor that inhibits the replication of diverse viruses. By virtue of its C-terminal HECT domain, HERC5 is the main cellular E3 ligase for conjugating ISG15 to substrates and localizes to polyribosomes to modify newly translated viral proteins, thereby disrupting key aspects of viral particle production. E3 ligase-independent antiviral activity has also been demonstrated towards HIV-1, where it inhibits the nuclear export of incompletely-spliced viral RNAs by a mechanism requiring its N-terminal RCC1-like domain (RLD). (PMID: 34572049)

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human		MBio; ISG15 Deficiency Enhances HIV-1 Infection by Accumulating Misfolded p53.; Authors - Edmund Osei Kuffour
human		Int J Mol Med; ISG15 inhibits cancer cell growth and promotes apoptosis.; Authors - Mei-Juan Zhou
human	CoIP	J Clin Invest; Posttranslational ISGylation of NLRP3 by HERCs enzymes facilitates inflammasome activation in models of inflammation; Authors - Ying Qin ; KD Validated
human		Cell Rep; Regulation of STING activity in DNA sensing by ISG15 modification; Authors - Chaohui Lin
human	CoIP	Cell Rep; ISGylation by HERCs facilitates STING activation; Authors - Ying Qin
human		Adv Sci (Weinh); Autophagy Deficiency Induced by SAT1 Potentiates Tumor Progression in Triple-Negative Breast Cancer; Authors - Wenwen Tian