HERC5 Polyclonal antibody

HERC5 Polyclonal Antibody for IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22692-1-AP

Synonyms

CEB1, CEBP1, Cyclin E binding protein 1, E3 ISG15 protein ligase HERC5, hect domain and RLD 5, HERC5



Tested Applications

Positive IP detected inHEK-293 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

The immunogen of 22692-1-AP is HERC5 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HERC5 fusion protein Ag18449
Full Name hect domain and RLD 5
Calculated molecular weight 1024 aa, 117 kDa
Observed molecular weight 117 kDa
GenBank accession numberBC140716
Gene symbol HERC5
Gene ID (NCBI) 51191
Conjugate Unconjugated
Form Liquid
Purification Method Antigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for HERC5 antibody 22692-1-APDownload protocol
IP protocol for HERC5 antibody 22692-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Int J Mol Med

ISG15 inhibits cancer cell growth and promotes apoptosis.

Authors - Mei-Juan Zhou
humanWB

MBio

ISG15 Deficiency Enhances HIV-1 Infection by Accumulating Misfolded p53.

Authors - Edmund Osei Kuffour