HIC2 Recombinant monoclonal antibody, PBS Only

• 

  IF Staining of HepG2 using 83162-7-RR (same clone as 83162-7-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HIC2 antibody (83162-7-RR, Clone: 230177E8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83162-7-PBS in a different storage buffer formulation.

• 

  IF Staining of U2OS using 83162-7-RR (same clone as 83162-7-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using HIC2 antibody (83162-7-RR, Clone: 230177E8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83162-7-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 83162-7-RR (same clone as 83162-7-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Hic2 Recombinant Antibody (83162-7-RR, Clone:230177E8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83162-7-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83162-7-RR

  Biolayer interferometry (BLl) kinetic assays of 83162-7-RR against Human HIC2 were performed. The affinity constant is 0.527 nM.

HIC2 Uni-rAb^® Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA

Cat No. 83162-7-PBS

Clone No.230177E8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Indirect ELISA

Synonyms

Hic-3, Hic-2, HIC1-related gene on chromosome 22 protein, Hic 3, Hic 2

---

Product Information

83162-7-PBS targets HIC2 in IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag18789

  Product name: Recombinant human HIC2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 161-430 aa of BC094787

  Sequence: RSQRLSTASVIQARYQGLVDGRKGAHAPQELPQAKGSDDELFLGGSNQDSVQGLGRAVCPAGGEAGLGGCSSSTNGSSGGCEQELGLDLSKKSPPLPPATPGPHLTPDDAAQLSDSQHGSPPAASAPPVANSASYSELGGTPDEPMDLEGAEDNHLSLLEAPGGQPRKSLRHSTRKKEWGKKEPVAGSPFERREAGPKGPCPGEEGEGVGDRVPNGILASGAGPSGPYGEPPYPCKEEEENGKDASEDSAQSGSEGGSGHASAHYMYRQE

• Full Name: hypermethylated in cancer 2
• Calculated Molecular Weight: 615 aa, 66 kDa
• GenBank Accession Number: BC094787
• Gene Symbol: HIC2
• Gene ID (NCBI): 23119
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q96JB3
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

HIC2 (hypermethylated in cancer 2) is a transcription factor related to the tumor suppressor HIC1, required for normal cardiac development and lost in distal variants of 22q11 Deletion Syndrome (PMID: 29061339). HIC2 is also a POK family protein, and the amino acid sequence of its BTB/POZ and zinc-finger domains are similar to those of HIC1(PMID: 31127867). Studies have shown that HIC2 plays a role in suppressing fetal genes for normal development of the heart and circulatory system, in embryogenesis (PMID: 24748541).