|Positive WB detected in||HeLa cells|
|Positive IF detected in||Cobalt Chloride treated HeLa cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
66730-1-Ig targets HIF1a in WB, IHC, IF,ELISA applications and shows reactivity with Human samples.
|Host / Isotype||Mouse / IgG1|
|Immunogen||HIF1a fusion protein Ag15198|
|Full Name||hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)|
|Calculated molecular weight||826 aa, 93 kDa|
|Observed molecular weight||120 kDa|
|GenBank accession number||BC012527|
|Gene ID (NCBI)||3091|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
HIF1a, the major regulator of the cellular responses to hypoxia, consists of an oxygen-sensitive subunit, HIF1 alpha (HIF1A), and an oxygen-insensitive subunit, HIF1 beta (arylhydrocarbon receptor nuclear transporter [ARNT]). Under normal oxygen conditions, HIF1a is continuously produced and destroyed, in a process involving hydroxylation, interaction with von Hippel-Lindau (VHL) protein, polyubiquitylation and subsequent proteasomal degradation. Under hypoxic conditions, hydroxylation is impaired and HIF1a is stabilized. HIF1a localizes in cytoplasm in normoxia, but it can translocate into nuclear in response to hypoxia. The calculated molecular weight of HIF1a is 93 kDa, but the modified protein HIF1a is about 110-120kDa (PMID: 11698256, .PMID: 7539918). .
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Diane (Verified Customer) (02-02-2021)
incubated over night 4 degrees C. 1:2500 HRP conjugated secondary. Opti-4CN substrate kit for detection. Also tried ICC on cytospins so ran out of trial size before fully optimized but has potential to provide the information that I am needing.