|Positive WB detected in
|HeLa cells, Cobalt Chloride treated HeLa cells, Cobalt Chloride treated HepG2 cells
|Western Blot (WB)
|WB : 1:2000-1:10000
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
66730-1-Ig targets HIF-1 alpha in WB, IP, IHC, IF, ELISA applications and shows reactivity with Human samples.
|Host / Isotype
|Mouse / IgG1
|HIF-1 alpha fusion protein Ag15198
|hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
|Calculated molecular weight
|826 aa, 93 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Protein A purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
HIF1a, the major regulator of the cellular responses to hypoxia, consists of an oxygen-sensitive subunit, HIF1 alpha (HIF1A), and an oxygen-insensitive subunit, HIF1 beta (arylhydrocarbon receptor nuclear transporter [ARNT]). Under normal oxygen conditions, HIF1a is continuously produced and destroyed, in a process involving hydroxylation, interaction with von Hippel-Lindau (VHL) protein, polyubiquitylation and subsequent proteasomal degradation. Under hypoxic conditions, hydroxylation is impaired and HIF1a is stabilized. HIF1a localizes in cytoplasm in normoxia, but it can translocate into nuclear in response to hypoxia. The calculated molecular weight of HIF1a is 93 kDa, but the modified protein HIF1a is about 110-120kDa (PMID: 11698256, .PMID: 7539918). .
Adv Sci (Weinh)
Transgelin Promotes Glioblastoma Stem Cell Hypoxic Responses and Maintenance Through p53 Acetylation
J Immunother Cancer
TSH-TSHR axis promotes tumor immune evasion.
ITGB2-mediated metabolic switch in CAFs promotes OSCC proliferation by oxidation of NADH in mitochondrial oxidative phosphorylation system.
Oxid Med Cell Longev
Chitosan Oligosaccharides Alleviate H2O2-stimulated Granulosa Cell Damage via HIF-1α Signaling Pathway.
J Med Chem
Potent Inhibition of HIF1α and p300 Interaction by a Constrained Peptide Derived from CITED2.
J Clin Endocrinol Metab
Targeting tumor hypoxia inhibits aggressive phenotype of dedifferentiated thyroid cancer
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
balawant (Verified Customer) (12-20-2022)
I have used this antibody for western blot and it is working great
Diane (Verified Customer) (02-02-2021)
incubated over night 4 degrees C. 1:2500 HRP conjugated secondary. Opti-4CN substrate kit for detection. Also tried ICC on cytospins so ran out of trial size before fully optimized but has potential to provide the information that I am needing.