HIF-1 alpha Monoclonal antibody

HIF-1 alpha Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1


Human and More (1)







Cat no : 66730-1-Ig


ARNT interacting protein, bHLHe78, HIF 1 alpha, HIF 1alpha, HIF 1α, HIF1, HIF1 ALPHA, HIF-1 alpha, HIF1A, HIF1α, Member of PAS protein 1, MOP1, PASD8

Tested Applications

Positive WB detected inHeLa cells, Cobalt Chloride treated HeLa cells, Cobalt Chloride treated HepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

66730-1-Ig targets HIF-1 alpha in WB, IP, IHC, IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen HIF-1 alpha fusion protein Ag15198
Full Name hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
Calculated molecular weight 826 aa, 93 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC012527
Gene symbol HIF1A
Gene ID (NCBI) 3091
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HIF1a, the major regulator of the cellular responses to hypoxia, consists of an oxygen-sensitive subunit, HIF1 alpha (HIF1A), and an oxygen-insensitive subunit, HIF1 beta (arylhydrocarbon receptor nuclear transporter [ARNT]). Under normal oxygen conditions, HIF1a is continuously produced and destroyed, in a process involving hydroxylation, interaction with von Hippel-Lindau (VHL) protein, polyubiquitylation and subsequent proteasomal degradation. Under hypoxic conditions, hydroxylation is impaired and HIF1a is stabilized. HIF1a localizes in cytoplasm in normoxia, but it can translocate into nuclear in response to hypoxia. The calculated molecular weight of HIF1a is 93 kDa, but the modified protein HIF1a is about 110-120kDa (PMID: 11698256, .PMID: 7539918). .


Product Specific Protocols
WB protocol for HIF-1 alpha antibody 66730-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Adv Sci (Weinh)

Transgelin Promotes Glioblastoma Stem Cell Hypoxic Responses and Maintenance Through p53 Acetylation

Authors - Huan Li

J Immunother Cancer

TSH-TSHR axis promotes tumor immune evasion.

Authors - Zhenghao Wu


ITGB2-mediated metabolic switch in CAFs promotes OSCC proliferation by oxidation of NADH in mitochondrial oxidative phosphorylation system.

Authors - Xiaoxin Zhang

Oxid Med Cell Longev

Chitosan Oligosaccharides Alleviate H2O2-stimulated Granulosa Cell Damage via HIF-1α Signaling Pathway.

Authors - Ziwei Yang

J Med Chem

Potent Inhibition of HIF1α and p300 Interaction by a Constrained Peptide Derived from CITED2.

Authors - Xuan Qin

J Clin Endocrinol Metab

Targeting tumor hypoxia inhibits aggressive phenotype of dedifferentiated thyroid cancer

Authors - Ben Ma


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


balawant (Verified Customer) (12-20-2022)

I have used this antibody for western blot and it is working great

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: renal Cancer cell line

Diane (Verified Customer) (02-02-2021)

incubated over night 4 degrees C. 1:2500 HRP conjugated secondary. Opti-4CN substrate kit for detection. Also tried ICC on cytospins so ran out of trial size before fully optimized but has potential to provide the information that I am needing.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1250
  • Cell Tissue Type: Cell lysates glioblastoma primary cells
HIF-1 alpha Antibody Western Blot validation (1:1250 dilution) in Cell lysates glioblastoma primary cells (Cat no:66730-1-Ig)