HIRIP3 Recombinant antibody

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  WB analysis using 85041-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85041-3-RR (HIRIP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 85041-3-RR

  WB result of HIRIP3 antibody (85041-3-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HIRIP3 transfected HEK-293 cells.

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  IF Staining of HeLa using 85041-3-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HIRIP3 antibody (85041-3-RR, Clone: 242708G7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• Featured Product
• KD/KO Validated

HIRIP3 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 85041-3-RR

Clone No.242708G7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

242708G7, HIRA-interacting protein 3

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, HL-60 cells, Jurkat cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85041-3-RR targets HIRIP3 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: HIRIP3 fusion protein Ag6978
• Full Name: HIRA interacting protein 3
• Calculated Molecular Weight: 62 kDa
• Observed Molecular Weight: 90 kDa
• GenBank Accession Number: BC000588
• Gene Symbol: HIRIP3
• Gene ID (NCBI): 8479
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9BW71
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The HIRIP3 protein had been identified from its interaction with the HIRA histone chaperone. It may play a role in chromatin function and histone metabolism via its interaction with HIRA and histones.