Histone H1.2 Polyclonal antibody

Histone H1.2 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 15446-1-AP

Synonyms

H1.2, H1F2, HIST1H1C, histone, histone cluster 1, H1c, Histone H1.2, Histone H1d

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Validation Data Gallery

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  WB analysis of Jurkat using 15446-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15446-1-AP (HIST1H1C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 15446-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 15446-1-AP (HIST1H1C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 15446-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 15446-1-AP (HIST1H1C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 15446-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 15446-1-AP (HIST1H1C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of MCF-7 using 15446-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed MCF-7 cells using 15446-1-AP (Histone H1.2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: Jurkat cells, human testis tissue, L02 cells, MCF-7 cells
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below

Product Information

15446-1-AP targets Histone H1.2 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Histone H1.2 fusion protein Ag7607
• Full Name: histone cluster 1, H1c
• Calculated Molecular Weight: 21 kDa
• Observed Molecular Weight: 32-33 kDa
• GenBank Accession Number: BC002649
• Gene Symbol: Histone H1.2
• Gene ID (NCBI): 3006
• RRID: AB_10858631
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Immunity; The Transcription Factor Runx3 Establishes Chromatin Accessibility of cis-Regulatory Landscapes that Drive Memory Cytotoxic T Lymphocyte Formation.; Authors - Dapeng Wang
human	WB	Gastroenterology; Regulator of Calcineurin 1 Gene Isoform 4, Downregulated in Hepatocellular Carcinoma, Prevents Proliferation, Migration, and Invasive Activity of Cancer Cells and Growth of Orthotopic Tumors by Inhibiting Nuclear Translocation of NFAT1.; Authors - Haojie Jin
human	WB	Nat Commun; Long non-coding RNA GAS5 controls human embryonic stem cell self-renewal by maintaining NODAL signalling.; Authors - Chen Xu
human	WB	Cell Physiol Biochem; Tumor-Secreted Exosomal miR-222 Promotes Tumor Progression via Regulating P27 Expression and Re-Localization in Pancreatic Cancer.; Authors - Zhonghu Li
human	WB	Stem Cell Res Ther; Exosomal SNHG16 secreted by CSCs promotes glioma development via TLR7.; Authors - Ruijie Zhang
human	WB	Oxid Med Cell Longev; Knockout of Mpv17-Like Protein (M-LPH) Gene in Human Hepatoma Cells Results in Impairment of mtDNA Integrity through Reduction of TFAM, OGG1, and LIG3 at the Protein Levels.; Authors - Reiko Iida