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- KD/KO Validated
HLA class I ABC Monoclonal antibody
HLA class I ABC Monoclonal Antibody for FC, IHC, WB, ELISA
Host / Isotype
Mouse / IgG2b
WB, IP, IHC, FC, ELISA
Cat no : 66013-1-Ig
Validation Data Gallery
|Positive WB detected in||HeLa cells, HepG2 cells, human white blood, NCCIT cells, pig spleen tissue, U-937 cells, Raji cells, HEK-293 cells, Jurkat cells|
|Positive IHC detected in||human tonsillitis tissue, human liver cancer tissue, human stomach cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in||Raji cells, HEK-293 cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunohistochemistry (IHC)||IHC : 1:1000-1:10000|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 1 publications below|
|WB||See 5 publications below|
|IHC||See 1 publications below|
|IP||See 1 publications below|
The immunogen of 66013-1-Ig is HLA class I ABC Fusion Protein expressed in E. coli.
|Tested Reactivity||human, pig|
|Cited Reactivity||human, pig|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||HLA class I ABC fusion protein Ag7488|
|Full Name||major histocompatibility complex, class I, A|
|Calculated molecular weight||41 kDa|
|Observed molecular weight||40-44 kDa|
|GenBank accession number||BC003069|
|Gene ID (NCBI)||3105|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). This class I molecules are polymorphic membrane glycoproteins composed of a heavy (alpha) chain (44 kDa) which is encoded by a HLA class I gene (HLA-A, B or C), and β2-microglobulin light (beta) chain (12 kDa). They are involved in the presentation of foreign antigens to the immune system. (PMID: 667938; 3375250)
|Product Specific Protocols|
|WB protocol for HLA class I ABC antibody 66013-1-Ig||Download protocol|
|IHC protocol for HLA class I ABC antibody 66013-1-Ig||Download protocol|
|FC protocol for HLA class I ABC antibody 66013-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
Combination epidermal growth factor receptor variant III peptide-pulsed dendritic cell vaccine with miR-326 results in enhanced killing on EGFRvIII-positive cells.
J Biol Chem
The transferrin receptor CD71 regulates type II CD38, revealing tight topological compartmentalization of intracellular cyclic ADP-ribose production.
Asian Pac J Cancer Prev
Loss of heterozygosity at 6p21 and HLA class I expression in esophageal squamous cell carcinomas in China.
J Tissue Eng Regen Med
Sequential hydrophile and lipophile solubilization as an efficient method for decellularization of porcine aortic valve leaflets: structure, mechanical property and biocompatibility study.
CD80 expression promotes immune surveillance in Barrett's metaplasia.
Inhibition of major histocompatibility complex-I antigen presentation by sarbecovirus ORF7a proteins.