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HLA class I ABC Monoclonal antibody

HLA class I ABC Monoclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b


human, pig







Cat no : 66013-1-Ig


HLA A, HLA class I, HLA class I (HLA-A), HLA class I ABC, HLA-A, MHC class I antigen A*3

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, human white blood, NCCIT cells, pig spleen tissue, U-937 cells, Raji cells, HEK-293 cells, Jurkat cells
Positive IHC detected inhuman tonsillitis tissue, human liver cancer tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inRaji cells, HEK-293 cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:10000
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 66013-1-Ig is HLA class I ABC Fusion Protein expressed in E. coli.

Tested Reactivity human, pig
Cited Reactivityhuman, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen HLA class I ABC fusion protein Ag7488
Full Name major histocompatibility complex, class I, A
Calculated molecular weight 41 kDa
Observed molecular weight 40-44 kDa
GenBank accession numberBC003069
Gene symbol HLA-A
Gene ID (NCBI) 3105
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). This class I molecules are polymorphic membrane glycoproteins composed of a heavy (alpha) chain (44 kDa) which is encoded by a HLA class I gene (HLA-A, B or C), and β2-microglobulin light (beta) chain (12 kDa). They are involved in the presentation of foreign antigens to the immune system. (PMID: 667938; 3375250)


Product Specific Protocols
WB protocol for HLA class I ABC antibody 66013-1-IgDownload protocol
IHC protocol for HLA class I ABC antibody 66013-1-IgDownload protocol
FC protocol for HLA class I ABC antibody 66013-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols




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