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CoraLite®488-conjugated HLA class I ABC Monoclonal antibody

HLA class I ABC Monoclonal Antibody for FC, IF

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig

Applications

IF, FC

Conjugate

CoraLite®488 Fluorescent Dye

CloneNo.

5C5B7

Cat no : CL488-66013

Synonyms

HLA A, HLAA, HLA-A, MHC class I antigen A*3



Tested Applications

Positive IF detected inhuman tonsillitis tissue
Positive FC detected inRaji cells
For other applications, we recommend the unconjugated version of this antibody, 66013-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of CL488-66013 is HLA class I ABC Fusion Protein expressed in E. coli.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen HLA class I ABC fusion protein Ag7488
Full Name major histocompatibility complex, class I, A
Calculated molecular weight 41 kDa
Observed molecular weight 40-44 kDa
GenBank accession numberBC003069
Gene symbol HLA-A
Gene ID (NCBI) 3105
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission maxima wavelengths488 nm/515 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). This class I molecules are polymorphic membrane glycoproteins composed of a heavy (alpha) chain (44 kDa) which is encoded by a HLA class I gene (HLA-A, B or C), and β2-microglobulin light (beta) chain (12 kDa). They are involved in the presentation of foreign antigens to the immune system. (PMID: 667938; 3375250)

Protocols

Product Specific Protocols
IF protocol for CL488 HLA class I ABC antibody CL488-66013Download protocol
FC protocol for CL488 HLA class I ABC antibody CL488-66013Download protocol
Standard Protocols
Click here to view our Standard Protocols